Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c Mice
Self-assembling ferritin nanoparticle technology is a widely used vaccine development platform for enhancing the efficacy of subunit vaccines by displaying multiple antigens on nanocages. The dengue virus (DENV) envelope domain III (EDIII) protein, the most promising antigen for DENV, has been appli...
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| author | M.S.B.W.T.M. Nipuna Sudaraka Tennakoon Kyoung-Ho Lee Hye-Mi Lee Jae-Yeon Park Hyun-Jin Shin |
| author_facet | M.S.B.W.T.M. Nipuna Sudaraka Tennakoon Kyoung-Ho Lee Hye-Mi Lee Jae-Yeon Park Hyun-Jin Shin |
| author_sort | M.S.B.W.T.M. Nipuna Sudaraka Tennakoon |
| collection | DOAJ |
| description | Self-assembling ferritin nanoparticle technology is a widely used vaccine development platform for enhancing the efficacy of subunit vaccines by displaying multiple antigens on nanocages. The dengue virus (DENV) envelope domain III (EDIII) protein, the most promising antigen for DENV, has been applied in vaccine development, and it is essential to evaluate the relative immunogenicity of the EDIII protein and EDIII-conjugated ferritin to show the efficiency of the ferritin delivery system compared with EDIII. In this study, we optimized the conditions for the expression of the EDIII protein in <i>E. coli</i>, protein purification, and refolding, and these optimization techniques were applied for the purification of EDIII ferritin nanoparticles. Thus, purified DENV2 EDIII and EDIII human ferritin heavy chain nanoparticles were immunized intramuscularly into BALB/c mice without an adjuvant, and the immunogenicity was analyzed using IgG ELISA and a serum-neutralizing assay. Purified, properly refolded, aggregate-free EDIII and EDIII ferritin proteins were obtained, and ferritin nanoparticles were identified using an electron microscope. By analyzing the immunogenicity of mouse serum, EDIII ferritin generated significantly higher IgG responses and neutralizing activity than EDIII-immunized mice. The IgG ELISA results confirmed that EDIII ferritin can induce a significantly higher IgG titer (O.D.:1.8) than EDIII (O.D.:0.05). Furthermore, EDIII ferritin produced a neutralizing titer of 1:68, whereas EDIII protein produced an average titer of 1:16, which is the serum dilution that inhibited 90% of the viruses. The longevity of the immune responses was analyzed using the serum obtained 2 months after the final immunization, and the results confirmed that EDIII ferritin induced constant immunity throughout the period. |
| format | Article |
| id | doaj-art-2424360c39934db28241a1bf8bc8955f |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-2424360c39934db28241a1bf8bc8955f2025-01-24T13:52:41ZengMDPI AGViruses1999-49152025-01-0117112910.3390/v17010129Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c MiceM.S.B.W.T.M. Nipuna Sudaraka Tennakoon0Kyoung-Ho Lee1Hye-Mi Lee2Jae-Yeon Park3Hyun-Jin Shin4Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of KoreaLaboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of KoreaLaboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of KoreaLaboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of KoreaLaboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of KoreaSelf-assembling ferritin nanoparticle technology is a widely used vaccine development platform for enhancing the efficacy of subunit vaccines by displaying multiple antigens on nanocages. The dengue virus (DENV) envelope domain III (EDIII) protein, the most promising antigen for DENV, has been applied in vaccine development, and it is essential to evaluate the relative immunogenicity of the EDIII protein and EDIII-conjugated ferritin to show the efficiency of the ferritin delivery system compared with EDIII. In this study, we optimized the conditions for the expression of the EDIII protein in <i>E. coli</i>, protein purification, and refolding, and these optimization techniques were applied for the purification of EDIII ferritin nanoparticles. Thus, purified DENV2 EDIII and EDIII human ferritin heavy chain nanoparticles were immunized intramuscularly into BALB/c mice without an adjuvant, and the immunogenicity was analyzed using IgG ELISA and a serum-neutralizing assay. Purified, properly refolded, aggregate-free EDIII and EDIII ferritin proteins were obtained, and ferritin nanoparticles were identified using an electron microscope. By analyzing the immunogenicity of mouse serum, EDIII ferritin generated significantly higher IgG responses and neutralizing activity than EDIII-immunized mice. The IgG ELISA results confirmed that EDIII ferritin can induce a significantly higher IgG titer (O.D.:1.8) than EDIII (O.D.:0.05). Furthermore, EDIII ferritin produced a neutralizing titer of 1:68, whereas EDIII protein produced an average titer of 1:16, which is the serum dilution that inhibited 90% of the viruses. The longevity of the immune responses was analyzed using the serum obtained 2 months after the final immunization, and the results confirmed that EDIII ferritin induced constant immunity throughout the period.https://www.mdpi.com/1999-4915/17/1/129dengueferritin nanoparticlesEDIII<i>Escherichia coli</i> expressionprotein aggregatesimmune responses |
| spellingShingle | M.S.B.W.T.M. Nipuna Sudaraka Tennakoon Kyoung-Ho Lee Hye-Mi Lee Jae-Yeon Park Hyun-Jin Shin Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c Mice Viruses dengue ferritin nanoparticles EDIII <i>Escherichia coli</i> expression protein aggregates immune responses |
| title | Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c Mice |
| title_full | Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c Mice |
| title_fullStr | Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c Mice |
| title_full_unstemmed | Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c Mice |
| title_short | Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c Mice |
| title_sort | optimization of conditions for expression of dengue serotype 2 ediii protein in i escherichia coli i and immune responses of adjuvant free ediii ferritin nanoparticles against dengue virus in balb c mice |
| topic | dengue ferritin nanoparticles EDIII <i>Escherichia coli</i> expression protein aggregates immune responses |
| url | https://www.mdpi.com/1999-4915/17/1/129 |
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