Biosynthesis of Crystalline Silver and Gold Nanoparticles by Extremophilic Yeasts
The biosynthesis of Ag and Au nanoparticles (NPs) was investigated using an extremophilic yeast strain isolated from acid mine drainage in Portugal. Three distinct studies were performed, namely, the growth of yeast strain in presence of metal ions, the use of yeast biomass for the metal nanoparticl...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2011/546074 |
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| _version_ | 1832545559908450304 |
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| author | Ana Mourato Mário Gadanho Ana R. Lino Rogério Tenreiro |
| author_facet | Ana Mourato Mário Gadanho Ana R. Lino Rogério Tenreiro |
| author_sort | Ana Mourato |
| collection | DOAJ |
| description | The biosynthesis of Ag and Au nanoparticles (NPs) was investigated using an extremophilic yeast strain isolated from acid mine drainage in Portugal. Three distinct studies were performed, namely, the growth of yeast strain in presence of metal ions, the use of yeast biomass for the metal nanoparticles synthesis, and of the supernatant obtained after 24-hour incubation of yeast biomass in water. The extremophilic strain under study was able to grow up to an Ag ion concentration of 1.5 mM whereas an increase of Au ion concentration over 0.09 mM caused a strong inhibitory effect. A successful route for the metal NPs synthesis was obtained using the yeast biomass. When the washed yeast cells were in contact with Ag or Au solutions, AgNPs smaller than 20 nm were produced, as for the AuNPs diameter ranged from 30 to 100 nm, as determined through transmission electron microscopy and confirmed by energy-dispersive X-ray spectra. The supernatant-based strategy provided evidence that proteins were released to the medium by the yeasts, which could be responsible for the formation and stabilisation of the Ag NPs, although the involvement of the cell wall seems fundamental for AuNPs synthesis. |
| format | Article |
| id | doaj-art-240e9cf72391410bb444bc6775abbef1 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-240e9cf72391410bb444bc6775abbef12025-02-03T07:25:26ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2011-01-01201110.1155/2011/546074546074Biosynthesis of Crystalline Silver and Gold Nanoparticles by Extremophilic YeastsAna Mourato0Mário Gadanho1Ana R. Lino2Rogério Tenreiro3Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Edificio C8, Campus da FCUL, Campo Grande, 1749-016 Lisboa, PortugalCentro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Faculdade de Ciências, Universidade de Lisboa, Edificio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, PortugalCentro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Edificio C8, Campus da FCUL, Campo Grande, 1749-016 Lisboa, PortugalCentro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Faculdade de Ciências, Universidade de Lisboa, Edificio ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, PortugalThe biosynthesis of Ag and Au nanoparticles (NPs) was investigated using an extremophilic yeast strain isolated from acid mine drainage in Portugal. Three distinct studies were performed, namely, the growth of yeast strain in presence of metal ions, the use of yeast biomass for the metal nanoparticles synthesis, and of the supernatant obtained after 24-hour incubation of yeast biomass in water. The extremophilic strain under study was able to grow up to an Ag ion concentration of 1.5 mM whereas an increase of Au ion concentration over 0.09 mM caused a strong inhibitory effect. A successful route for the metal NPs synthesis was obtained using the yeast biomass. When the washed yeast cells were in contact with Ag or Au solutions, AgNPs smaller than 20 nm were produced, as for the AuNPs diameter ranged from 30 to 100 nm, as determined through transmission electron microscopy and confirmed by energy-dispersive X-ray spectra. The supernatant-based strategy provided evidence that proteins were released to the medium by the yeasts, which could be responsible for the formation and stabilisation of the Ag NPs, although the involvement of the cell wall seems fundamental for AuNPs synthesis.http://dx.doi.org/10.1155/2011/546074 |
| spellingShingle | Ana Mourato Mário Gadanho Ana R. Lino Rogério Tenreiro Biosynthesis of Crystalline Silver and Gold Nanoparticles by Extremophilic Yeasts Bioinorganic Chemistry and Applications |
| title | Biosynthesis of Crystalline Silver and Gold Nanoparticles by Extremophilic Yeasts |
| title_full | Biosynthesis of Crystalline Silver and Gold Nanoparticles by Extremophilic Yeasts |
| title_fullStr | Biosynthesis of Crystalline Silver and Gold Nanoparticles by Extremophilic Yeasts |
| title_full_unstemmed | Biosynthesis of Crystalline Silver and Gold Nanoparticles by Extremophilic Yeasts |
| title_short | Biosynthesis of Crystalline Silver and Gold Nanoparticles by Extremophilic Yeasts |
| title_sort | biosynthesis of crystalline silver and gold nanoparticles by extremophilic yeasts |
| url | http://dx.doi.org/10.1155/2011/546074 |
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