Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD)
Background. Metabolic surgery procedures are designed not only for sustained weight loss but also for achieving positive metabolic changes, including improved glucose tolerance and insulin sensitivity, along with an increase in energy expenditure. Based on recent findings, the present study focuses...
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| Format: | Article |
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Wiley
2021-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2021/6699923 |
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| author | Tomasz Sawczyn Dominika Stygar Katarzyna Nabrdalik Michał Kukla Oliwia Masri Łukasz Magrowski Wojciech Karcz Jerzy Jochem |
| author_facet | Tomasz Sawczyn Dominika Stygar Katarzyna Nabrdalik Michał Kukla Oliwia Masri Łukasz Magrowski Wojciech Karcz Jerzy Jochem |
| author_sort | Tomasz Sawczyn |
| collection | DOAJ |
| description | Background. Metabolic surgery procedures are designed not only for sustained weight loss but also for achieving positive metabolic changes, including improved glucose tolerance and insulin sensitivity, along with an increase in energy expenditure. Based on recent findings, the present study focuses on the relationship between the effects of ileal transposition (IT), high-fat diet (HFD), and selected markers of lipid metabolism and inflammation. Methods. Forty-eight male rats were divided into two groups: HFD and control diet (CD) fed rats. After eight weeks, animals in each group were randomly assigned to two types of surgery: IT and SHAM. Thereafter, fifty percent of the animals in the HFD and CD groups had their diets changed, while the remaining half maintained their presurgery diets. Eight weeks after surgery, plasma levels of ANGPTL8, PTX3, leptin, and adiponectin were assessed. Results. The IT group pre- and postoperatively maintained on the HFD showed higher ANGPTL8 level compared to SHAM operated animals (p=0.0041). The effect of IT on PTX3 level in the group pre- and postoperatively maintained on a CD was not significant, and there were no differences compared to SHAM. Only the postoperative diet change to HFD increased PTX3 level in the IT operated animals (p=0.0002). The IT group had increased plasma adiponectin (p=0.026) and leptin (p=0.0027) levels after dietary change to HFD compared to IT rats fed CD. Conclusions. This study indicates that the outcomes of metabolic surgery can be greatly modified by HFD. The effects of the IT procedure in this experiment are ambiguous and do not provide a clear answer as to whether or not they are beneficial. |
| format | Article |
| id | doaj-art-23e1a7d83b8946f1bfcba4ee2fb63576 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | International Journal of Endocrinology |
| spelling | doaj-art-23e1a7d83b8946f1bfcba4ee2fb635762025-02-03T01:05:30ZengWileyInternational Journal of Endocrinology1687-83371687-83452021-01-01202110.1155/2021/66999236699923Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD)Tomasz Sawczyn0Dominika Stygar1Katarzyna Nabrdalik2Michał Kukla3Oliwia Masri4Łukasz Magrowski5Wojciech Karcz6Jerzy Jochem7Department of Physiology, Faculty of Medical Sciences, Zabrze, Medical University of Silesia, Katowice, PolandDepartment of Physiology, Faculty of Medical Sciences, Zabrze, Medical University of Silesia, Katowice, PolandDepartment of Internal Medicine, Diabetology and Nephrology, Zabrze, Medical University of Silesia, Katowice, PolandDepartment of Internal Medicine and Geriatrics, Jagiellonian University Medical College, Krakow, PolandDepartment of Physiology, Faculty of Medical Sciences, Zabrze, Medical University of Silesia, Katowice, PolandDepartment of Physiology, Faculty of Medical Sciences, Zabrze, Medical University of Silesia, Katowice, PolandDepartment of General, Visceral, Transplantation and Vascular Surgery, Hospital of the Ludwig Maximilian University, Munich, GermanyDepartment of Physiology, Faculty of Medical Sciences, Zabrze, Medical University of Silesia, Katowice, PolandBackground. Metabolic surgery procedures are designed not only for sustained weight loss but also for achieving positive metabolic changes, including improved glucose tolerance and insulin sensitivity, along with an increase in energy expenditure. Based on recent findings, the present study focuses on the relationship between the effects of ileal transposition (IT), high-fat diet (HFD), and selected markers of lipid metabolism and inflammation. Methods. Forty-eight male rats were divided into two groups: HFD and control diet (CD) fed rats. After eight weeks, animals in each group were randomly assigned to two types of surgery: IT and SHAM. Thereafter, fifty percent of the animals in the HFD and CD groups had their diets changed, while the remaining half maintained their presurgery diets. Eight weeks after surgery, plasma levels of ANGPTL8, PTX3, leptin, and adiponectin were assessed. Results. The IT group pre- and postoperatively maintained on the HFD showed higher ANGPTL8 level compared to SHAM operated animals (p=0.0041). The effect of IT on PTX3 level in the group pre- and postoperatively maintained on a CD was not significant, and there were no differences compared to SHAM. Only the postoperative diet change to HFD increased PTX3 level in the IT operated animals (p=0.0002). The IT group had increased plasma adiponectin (p=0.026) and leptin (p=0.0027) levels after dietary change to HFD compared to IT rats fed CD. Conclusions. This study indicates that the outcomes of metabolic surgery can be greatly modified by HFD. The effects of the IT procedure in this experiment are ambiguous and do not provide a clear answer as to whether or not they are beneficial.http://dx.doi.org/10.1155/2021/6699923 |
| spellingShingle | Tomasz Sawczyn Dominika Stygar Katarzyna Nabrdalik Michał Kukla Oliwia Masri Łukasz Magrowski Wojciech Karcz Jerzy Jochem Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD) International Journal of Endocrinology |
| title | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD) |
| title_full | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD) |
| title_fullStr | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD) |
| title_full_unstemmed | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD) |
| title_short | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet (HFD) |
| title_sort | effect of ileal transposition it on angiopoietin like protein 8 angptl8 and pentraxin ptx3 plasma level in sprague dawley rats fed high fat diet hfd |
| url | http://dx.doi.org/10.1155/2021/6699923 |
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