Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib Families
Microsatellite markers are widely used in aquaculture for genetic analysis and breeding programs, but challenges such as segregation distortion and allelic instability can impact their effectiveness in parentage verification and inheritance studies. This study evaluated 15 microsatellite loci in sev...
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MDPI AG
2025-01-01
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| author | Songhyun Gwon Eunjeong Kim Wonse Lee Jisung Han Yoonkwon Nam |
| author_facet | Songhyun Gwon Eunjeong Kim Wonse Lee Jisung Han Yoonkwon Nam |
| author_sort | Songhyun Gwon |
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| description | Microsatellite markers are widely used in aquaculture for genetic analysis and breeding programs, but challenges such as segregation distortion and allelic instability can impact their effectiveness in parentage verification and inheritance studies. This study evaluated 15 microsatellite loci in seven experimental olive flounder (<i>Paralichthys olivaceus</i>) families bred through 1:1 full-sibling crosses, assessing their utility for accurate parentage and inheritance stability. Parentage assignments were conducted within an expanded pool of 647 candidate parents (including the actual 14 parents), encompassing both closely related and moderately distant individuals. Despite increased genetic diversity, assignments maintained a high accuracy rate (99.6%), demonstrating marker robustness. Differences in delta values highlighted the influence of genetic backgrounds on assignment clarity, with some groups showing reduced distinctiveness in the expanded pool. Segregation distortion was observed at certain loci, deviating from Mendelian inheritance, likely due to meiotic drive and post-zygotic selection. These findings underscore the need for empirical validation of microsatellite loci for stable inheritance and reliable parentage in diverse breeding programs, especially with genetically similar spawners. |
| format | Article |
| id | doaj-art-23baca47137b4500ac7ae1ee06f92a47 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-23baca47137b4500ac7ae1ee06f92a472025-01-24T13:17:55ZengMDPI AGAnimals2076-26152025-01-0115217610.3390/ani15020176Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib FamiliesSonghyun Gwon0Eunjeong Kim1Wonse Lee2Jisung Han3Yoonkwon Nam4Faculty of Fisheries Life Sciences, Pukyong National University, Nam-Gu, Busan 48513, Republic of KoreaFaculty of Fisheries Life Sciences, Pukyong National University, Nam-Gu, Busan 48513, Republic of KoreaAll-in-One Gene Tech, Buk-Gu, Busan 46508, Republic of KoreaAll-in-One Gene Tech, Buk-Gu, Busan 46508, Republic of KoreaFaculty of Fisheries Life Sciences, Pukyong National University, Nam-Gu, Busan 48513, Republic of KoreaMicrosatellite markers are widely used in aquaculture for genetic analysis and breeding programs, but challenges such as segregation distortion and allelic instability can impact their effectiveness in parentage verification and inheritance studies. This study evaluated 15 microsatellite loci in seven experimental olive flounder (<i>Paralichthys olivaceus</i>) families bred through 1:1 full-sibling crosses, assessing their utility for accurate parentage and inheritance stability. Parentage assignments were conducted within an expanded pool of 647 candidate parents (including the actual 14 parents), encompassing both closely related and moderately distant individuals. Despite increased genetic diversity, assignments maintained a high accuracy rate (99.6%), demonstrating marker robustness. Differences in delta values highlighted the influence of genetic backgrounds on assignment clarity, with some groups showing reduced distinctiveness in the expanded pool. Segregation distortion was observed at certain loci, deviating from Mendelian inheritance, likely due to meiotic drive and post-zygotic selection. These findings underscore the need for empirical validation of microsatellite loci for stable inheritance and reliable parentage in diverse breeding programs, especially with genetically similar spawners.https://www.mdpi.com/2076-2615/15/2/176microsatellitesolive flounderparentage verificationsegregation distortion |
| spellingShingle | Songhyun Gwon Eunjeong Kim Wonse Lee Jisung Han Yoonkwon Nam Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib Families Animals microsatellites olive flounder parentage verification segregation distortion |
| title | Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib Families |
| title_full | Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib Families |
| title_fullStr | Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib Families |
| title_full_unstemmed | Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib Families |
| title_short | Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib Families |
| title_sort | parentage verification and segregation distortion patterns of microsatellite markers in olive flounder i paralichthys olivaceus i full sib families |
| topic | microsatellites olive flounder parentage verification segregation distortion |
| url | https://www.mdpi.com/2076-2615/15/2/176 |
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