Characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomics
Acute pancreatitis (AP) is a common gastrointestinal disorder with a high mortality rate. This study sought to identify the microbial community structure in patients with AP using metagenomics and culturomics. Compared to healthy controls, patients with AP exhibited a significant decrease in alpha d...
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025006231 |
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| author | Liang Gong Xue Li Li Ji Guorong Chen Ziying Han Lei Su Dong Wu |
| author_facet | Liang Gong Xue Li Li Ji Guorong Chen Ziying Han Lei Su Dong Wu |
| author_sort | Liang Gong |
| collection | DOAJ |
| description | Acute pancreatitis (AP) is a common gastrointestinal disorder with a high mortality rate. This study sought to identify the microbial community structure in patients with AP using metagenomics and culturomics. Compared to healthy controls, patients with AP exhibited a significant decrease in alpha diversity; a higher abundance of unclassified Enterococcus species (sp), Enterococcus faecium, and Enterococcus faecalis; and a lower abundance of Eubacterium rectale. A total of 336 isolates from 25 genera and 44 species were obtained by sample cultivation. The dominant species identified in patients with AP were Enterococcus faecium and Klebsiella grimontii, whereas those in the healthy controls were Enterococcus faecium, Escherichia coli, and Bacteroides faecis. Our research has contributed to the expanded understanding of the genome, diversity, and function of the intestinal microbiota in patients with AP and provided some reference for selecting culture medium and sample processing methods. |
| format | Article |
| id | doaj-art-23b7ea95f82b408293f5e49f3f3f94f3 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-23b7ea95f82b408293f5e49f3f3f94f32025-01-29T05:01:32ZengElsevierHeliyon2405-84402025-02-01113e42243Characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomicsLiang Gong0Xue Li1Li Ji2Guorong Chen3Ziying Han4Lei Su5Dong Wu6Department of Gastroenterology, Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Dongcheng District, No. 1 Shuaifuyuan, 100730, Beijing, China; NHC Key Laboratory of Human Disease Comparative Medicine, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, 100021, Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, 100021, Beijing, ChinaNanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University, Department of Geriatrics, 210008, Nanjing, Jiangsu, ChinaDepartment of Gastroenterology, China-Japan Friendship Hospital (Institute of Clinical Medical Sciences), Peking Union Medical College, Chinese Academy of Medical Sciences, 100029, Beijing, ChinaDepartment of Gastroenterology, Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Dongcheng District, No. 1 Shuaifuyuan, 100730, Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, 100021, Beijing, China; Corresponding author.Department of Gastroenterology, Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Dongcheng District, No. 1 Shuaifuyuan, 100730, Beijing, China; Department of Gastroenterology, The People's Hospital of Tibetan Autonomous Region, Lhasa, 850000, China; Corresponding author. Department of Gastroenterology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Dongcheng District, No.1 Shuaifuyuan, 100730, Beijing, China.Acute pancreatitis (AP) is a common gastrointestinal disorder with a high mortality rate. This study sought to identify the microbial community structure in patients with AP using metagenomics and culturomics. Compared to healthy controls, patients with AP exhibited a significant decrease in alpha diversity; a higher abundance of unclassified Enterococcus species (sp), Enterococcus faecium, and Enterococcus faecalis; and a lower abundance of Eubacterium rectale. A total of 336 isolates from 25 genera and 44 species were obtained by sample cultivation. The dominant species identified in patients with AP were Enterococcus faecium and Klebsiella grimontii, whereas those in the healthy controls were Enterococcus faecium, Escherichia coli, and Bacteroides faecis. Our research has contributed to the expanded understanding of the genome, diversity, and function of the intestinal microbiota in patients with AP and provided some reference for selecting culture medium and sample processing methods.http://www.sciencedirect.com/science/article/pii/S2405844025006231Acute pancreatitisGut microbiotaMetagenomicsCulturomics |
| spellingShingle | Liang Gong Xue Li Li Ji Guorong Chen Ziying Han Lei Su Dong Wu Characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomics Heliyon Acute pancreatitis Gut microbiota Metagenomics Culturomics |
| title | Characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomics |
| title_full | Characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomics |
| title_fullStr | Characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomics |
| title_full_unstemmed | Characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomics |
| title_short | Characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomics |
| title_sort | characterization and comparison of gut microbiota in patients with acute pancreatitis by metagenomics and culturomics |
| topic | Acute pancreatitis Gut microbiota Metagenomics Culturomics |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025006231 |
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