Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions
The generation of induced pluripotent stem cells (iPSCs) from somatic cells has enabled the possibility of providing unprecedented access to patient-specific iPSC cells for drug screening, disease modeling, and cell therapy applications. However, a major obstacle to the use of iPSC for therapeutic a...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/564612 |
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| _version_ | 1832564391532298240 |
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| author | Chad C. MacArthur Andrew Fontes Namritha Ravinder David Kuninger Jasmeet Kaur Matthew Bailey Antje Taliana Mohan C. Vemuri Pauline T. Lieu |
| author_facet | Chad C. MacArthur Andrew Fontes Namritha Ravinder David Kuninger Jasmeet Kaur Matthew Bailey Antje Taliana Mohan C. Vemuri Pauline T. Lieu |
| author_sort | Chad C. MacArthur |
| collection | DOAJ |
| description | The generation of induced pluripotent stem cells (iPSCs) from somatic cells has enabled the possibility of providing unprecedented access to patient-specific iPSC cells for drug screening, disease modeling, and cell therapy applications. However, a major obstacle to the use of iPSC for therapeutic applications is the potential of genomic modifications caused by insertion of viral transgenes in the cellular genome. A second concern is that reprogramming often requires the use of animal feeder layers and reagents that contain animal origin products, which hinder the generation of clinical-grade iPSCs. Here, we report the generation of iPSCs by an RNA Sendai virus vector that does not integrate into the cells genome, providing transgene-free iPSC line. In addition, reprogramming can be performed in feeder-free condition with StemPro hESC SFM medium and in xeno-free (XF) conditions. Generation of an integrant-free iPSCs generated in xeno-free media should facilitate the safe downstream applications of iPSC-based cell therapies. |
| format | Article |
| id | doaj-art-23b40ef6988444478ec3920231b476e2 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-23b40ef6988444478ec3920231b476e22025-02-03T01:11:09ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/564612564612Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free ConditionsChad C. MacArthur0Andrew Fontes1Namritha Ravinder2David Kuninger3Jasmeet Kaur4Matthew Bailey5Antje Taliana6Mohan C. Vemuri7Pauline T. Lieu8Primary and Stem Cell Systems, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USAPrimary and Stem Cell Systems, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USAMolecular and Cell Biology, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USAPrimary and Stem Cell Systems, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USAPrimary and Stem Cell Systems, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USAPrimary and Stem Cell Systems, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USAMolecular and Cell Biology, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USAPrimary and Stem Cell Systems, Life Technologies Corporation, 7335 Executive Way, Frederick, MD 21704, USAPrimary and Stem Cell Systems, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USAThe generation of induced pluripotent stem cells (iPSCs) from somatic cells has enabled the possibility of providing unprecedented access to patient-specific iPSC cells for drug screening, disease modeling, and cell therapy applications. However, a major obstacle to the use of iPSC for therapeutic applications is the potential of genomic modifications caused by insertion of viral transgenes in the cellular genome. A second concern is that reprogramming often requires the use of animal feeder layers and reagents that contain animal origin products, which hinder the generation of clinical-grade iPSCs. Here, we report the generation of iPSCs by an RNA Sendai virus vector that does not integrate into the cells genome, providing transgene-free iPSC line. In addition, reprogramming can be performed in feeder-free condition with StemPro hESC SFM medium and in xeno-free (XF) conditions. Generation of an integrant-free iPSCs generated in xeno-free media should facilitate the safe downstream applications of iPSC-based cell therapies.http://dx.doi.org/10.1155/2012/564612 |
| spellingShingle | Chad C. MacArthur Andrew Fontes Namritha Ravinder David Kuninger Jasmeet Kaur Matthew Bailey Antje Taliana Mohan C. Vemuri Pauline T. Lieu Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions Stem Cells International |
| title | Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions |
| title_full | Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions |
| title_fullStr | Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions |
| title_full_unstemmed | Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions |
| title_short | Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions |
| title_sort | generation of human induced pluripotent stem cells by a nonintegrating rna sendai virus vector in feeder free or xeno free conditions |
| url | http://dx.doi.org/10.1155/2012/564612 |
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