Functional Characterization of the <i>PoWHY1</i> Gene from <i>Platycladus orientalis</i> and Its Role in Abiotic Stress Tolerance in Transgenic <i>Arabidopsis thaliana</i>
The frequent occurrence of extreme weather conditions in the world has brought many unfavorable factors to plant growth, causing the growth and development of plants to be hindered and even leading to plant death, with abiotic stress hindering the growth and metabolism of plants due to severe uncont...
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2025-01-01
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| author | Chun Ou Zhiyu Dong Xudong Zheng Wenhui Cheng Ermei Chang Xiamei Yao |
| author_facet | Chun Ou Zhiyu Dong Xudong Zheng Wenhui Cheng Ermei Chang Xiamei Yao |
| author_sort | Chun Ou |
| collection | DOAJ |
| description | The frequent occurrence of extreme weather conditions in the world has brought many unfavorable factors to plant growth, causing the growth and development of plants to be hindered and even leading to plant death, with abiotic stress hindering the growth and metabolism of plants due to severe uncontrollability. The WHY1 transcription factor plays a critical role in regulating gene expression in plants, influencing chlorophyll biosynthesis, plant growth, and development, as well as responses to environmental stresses. The important role of the <i>PoWHY1</i> gene in regulating plant growth and adaptation to environmental stress has become a hot research topic. However, the mechanism of the <i>PoWHY1</i> gene in <i>Platycladus orientalis</i> under abiotic stress is still unclear. Here, the <i>PoWHY1</i> gene was analyzed bioinformatically using <i>P. orientalis</i> as study material, and the role of the gene against abiotic stress conditions in <i>Arabidopsis thaliana</i> was verified using transgenic technology. It was found that overexpression of <i>PoWHY1</i> increased seed germination, decreased malondialdehyde accumulation, increased proline content, and delayed the senescence process under salt stress. The expression levels of <i>JAZ1</i>, <i>LOX1</i>, <i>ABI1</i>, and <i>ABI2</i> were decreased, while the expression levels of <i>RAB18</i>, <i>APX1</i>, <i>GSTF6</i>, and <i>DREB2A</i> were increased, indicating that overexpression of <i>PoWHY1</i> enhanced the salt stress tolerance of <i>A. thaliana</i>. Furthermore, <i>PoWHY1</i> overexpression also increased drought tolerance in <i>A. thaliana</i>. From the above results, it can be concluded that maintaining high <i>PoWHY1</i> expression levels in the leaves of <i>P. orientalis</i> can improve their environmental adaptability. The results provide a scientific basis for understanding the gene function of the <i>PoWHY1</i> gene of <i>P. orientalis</i> under stress conditions and lay the foundation for further research on the function of the <i>PoWHY1</i> gene. |
| format | Article |
| id | doaj-art-23a197fc4be34e3189998991f3755cc6 |
| institution | Kabale University |
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| publishDate | 2025-01-01 |
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| spelling | doaj-art-23a197fc4be34e3189998991f3755cc62025-01-24T13:46:48ZengMDPI AGPlants2223-77472025-01-0114221810.3390/plants14020218Functional Characterization of the <i>PoWHY1</i> Gene from <i>Platycladus orientalis</i> and Its Role in Abiotic Stress Tolerance in Transgenic <i>Arabidopsis thaliana</i>Chun Ou0Zhiyu Dong1Xudong Zheng2Wenhui Cheng3Ermei Chang4Xiamei Yao5Fuyang Normal University—Funan Rural Revitalization Collaborative Technology Service Center, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, ChinaFuyang Normal University—Funan Rural Revitalization Collaborative Technology Service Center, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, ChinaFuyang Normal University—Funan Rural Revitalization Collaborative Technology Service Center, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, ChinaFuyang Normal University—Funan Rural Revitalization Collaborative Technology Service Center, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, ChinaResearch Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaSchool of Architecture and Urban Planning, Anhui Jianzhu University, Hefei 230601, ChinaThe frequent occurrence of extreme weather conditions in the world has brought many unfavorable factors to plant growth, causing the growth and development of plants to be hindered and even leading to plant death, with abiotic stress hindering the growth and metabolism of plants due to severe uncontrollability. The WHY1 transcription factor plays a critical role in regulating gene expression in plants, influencing chlorophyll biosynthesis, plant growth, and development, as well as responses to environmental stresses. The important role of the <i>PoWHY1</i> gene in regulating plant growth and adaptation to environmental stress has become a hot research topic. However, the mechanism of the <i>PoWHY1</i> gene in <i>Platycladus orientalis</i> under abiotic stress is still unclear. Here, the <i>PoWHY1</i> gene was analyzed bioinformatically using <i>P. orientalis</i> as study material, and the role of the gene against abiotic stress conditions in <i>Arabidopsis thaliana</i> was verified using transgenic technology. It was found that overexpression of <i>PoWHY1</i> increased seed germination, decreased malondialdehyde accumulation, increased proline content, and delayed the senescence process under salt stress. The expression levels of <i>JAZ1</i>, <i>LOX1</i>, <i>ABI1</i>, and <i>ABI2</i> were decreased, while the expression levels of <i>RAB18</i>, <i>APX1</i>, <i>GSTF6</i>, and <i>DREB2A</i> were increased, indicating that overexpression of <i>PoWHY1</i> enhanced the salt stress tolerance of <i>A. thaliana</i>. Furthermore, <i>PoWHY1</i> overexpression also increased drought tolerance in <i>A. thaliana</i>. From the above results, it can be concluded that maintaining high <i>PoWHY1</i> expression levels in the leaves of <i>P. orientalis</i> can improve their environmental adaptability. The results provide a scientific basis for understanding the gene function of the <i>PoWHY1</i> gene of <i>P. orientalis</i> under stress conditions and lay the foundation for further research on the function of the <i>PoWHY1</i> gene.https://www.mdpi.com/2223-7747/14/2/218<i>Platycladus orientalis</i><i>PoWHY1</i>gene expression and analysisabiotic stressesdrought |
| spellingShingle | Chun Ou Zhiyu Dong Xudong Zheng Wenhui Cheng Ermei Chang Xiamei Yao Functional Characterization of the <i>PoWHY1</i> Gene from <i>Platycladus orientalis</i> and Its Role in Abiotic Stress Tolerance in Transgenic <i>Arabidopsis thaliana</i> Plants <i>Platycladus orientalis</i> <i>PoWHY1</i> gene expression and analysis abiotic stresses drought |
| title | Functional Characterization of the <i>PoWHY1</i> Gene from <i>Platycladus orientalis</i> and Its Role in Abiotic Stress Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_full | Functional Characterization of the <i>PoWHY1</i> Gene from <i>Platycladus orientalis</i> and Its Role in Abiotic Stress Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_fullStr | Functional Characterization of the <i>PoWHY1</i> Gene from <i>Platycladus orientalis</i> and Its Role in Abiotic Stress Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | Functional Characterization of the <i>PoWHY1</i> Gene from <i>Platycladus orientalis</i> and Its Role in Abiotic Stress Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_short | Functional Characterization of the <i>PoWHY1</i> Gene from <i>Platycladus orientalis</i> and Its Role in Abiotic Stress Tolerance in Transgenic <i>Arabidopsis thaliana</i> |
| title_sort | functional characterization of the i powhy1 i gene from i platycladus orientalis i and its role in abiotic stress tolerance in transgenic i arabidopsis thaliana i |
| topic | <i>Platycladus orientalis</i> <i>PoWHY1</i> gene expression and analysis abiotic stresses drought |
| url | https://www.mdpi.com/2223-7747/14/2/218 |
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