Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity Traps
The current work demonstrates the design, characterization, and preparation of molecularly imprinted microspheres for the selective detection of myoglobin in serum samples. The suspension polymerization approach was applied for the preparation of myoglobin imprinted microspheres. For this purpose, N...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/4359892 |
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| _version_ | 1832562099849527296 |
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| author | Rüstem Keçili |
| author_facet | Rüstem Keçili |
| author_sort | Rüstem Keçili |
| collection | DOAJ |
| description | The current work demonstrates the design, characterization, and preparation of molecularly imprinted microspheres for the selective detection of myoglobin in serum samples. The suspension polymerization approach was applied for the preparation of myoglobin imprinted microspheres. For this purpose, N-methacryloylamino folic acid-Nd3+ (MAFol- Nd3+) was chosen as the complex functional monomer. The optimization studies were performed changing the medium pH, temperature, and myoglobin concentration. pH 7.0 was determined as the optimum value where the prepared imprinted microspheres displayed maximum binding for myoglobin. The maximum binding capacity was achieved as 623 mgg−1. In addition, the selectivity studies were conducted. The results confirmed that the imprinted microspheres showed great selectivity towards myoglobin in the existence of hemoglobin, cytochrome c, and lysozyme which were chosen as potentially competing proteins. |
| format | Article |
| id | doaj-art-2327c033ac6b48deb3c432caca6a5f05 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-2327c033ac6b48deb3c432caca6a5f052025-02-03T01:23:26ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792018-01-01201810.1155/2018/43598924359892Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity TrapsRüstem Keçili0Anadolu University, Yunus Emre Vocational School of Health Services, Department of Medical Services and Techniques, 26470 Eskisehir, TurkeyThe current work demonstrates the design, characterization, and preparation of molecularly imprinted microspheres for the selective detection of myoglobin in serum samples. The suspension polymerization approach was applied for the preparation of myoglobin imprinted microspheres. For this purpose, N-methacryloylamino folic acid-Nd3+ (MAFol- Nd3+) was chosen as the complex functional monomer. The optimization studies were performed changing the medium pH, temperature, and myoglobin concentration. pH 7.0 was determined as the optimum value where the prepared imprinted microspheres displayed maximum binding for myoglobin. The maximum binding capacity was achieved as 623 mgg−1. In addition, the selectivity studies were conducted. The results confirmed that the imprinted microspheres showed great selectivity towards myoglobin in the existence of hemoglobin, cytochrome c, and lysozyme which were chosen as potentially competing proteins.http://dx.doi.org/10.1155/2018/4359892 |
| spellingShingle | Rüstem Keçili Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity Traps International Journal of Analytical Chemistry |
| title | Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity Traps |
| title_full | Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity Traps |
| title_fullStr | Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity Traps |
| title_full_unstemmed | Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity Traps |
| title_short | Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity Traps |
| title_sort | selective recognition of myoglobin in biological samples using molecularly imprinted polymer based affinity traps |
| url | http://dx.doi.org/10.1155/2018/4359892 |
| work_keys_str_mv | AT rustemkecili selectiverecognitionofmyoglobininbiologicalsamplesusingmolecularlyimprintedpolymerbasedaffinitytraps |