Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy
Summary: Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photon microscope. We describe an approach for construc...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000061 |
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| author | Jian Zhou Fengling Du Fan Zhang Xianhui Zhang Yuanyuan Wu Lifang Zhang Ping Liao Tianqi Tu Jianhua Peng Tao Li Yong Jiang |
| author_facet | Jian Zhou Fengling Du Fan Zhang Xianhui Zhang Yuanyuan Wu Lifang Zhang Ping Liao Tianqi Tu Jianhua Peng Tao Li Yong Jiang |
| author_sort | Jian Zhou |
| collection | DOAJ |
| description | Summary: Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photon microscope. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. We then detail procedures for the preparation of a coverslip with a customized titanium ring and cranial window for two-photon microscopy scanning.For complete details on the use and execution of this protocol, please refer to Zhou et al. 1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-2323b10eb8fb41d181cd773021620652 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-2323b10eb8fb41d181cd7730216206522025-01-26T05:04:56ZengElsevierSTAR Protocols2666-16672025-03-0161103600Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopyJian Zhou0Fengling Du1Fan Zhang2Xianhui Zhang3Yuanyuan Wu4Lifang Zhang5Ping Liao6Tianqi Tu7Jianhua Peng8Tao Li9Yong Jiang10Department of Neurosurgery, the First Affiliated Hospital of Jinan University, Guangzhou, China; Department of Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, ChinaDepartment of Neonatology, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, ChinaSichuan Clinical Research Center for Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, ChinaSichuan Clinical Research Center for Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, ChinaSichuan Clinical Research Center for Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, ChinaDepartment of Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, China; Sichuan Clinical Research Center for Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, ChinaLaboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Center of Translational Medicine of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, ChinaDepartment of Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, China; Institute of Epigenetics and Brain Science, Southwest Medical University, Luzhou 646000, China; Corresponding authorDepartment of Anesthesiology, Laboratory of Mitochondrial Metabolism and Perioperative Medicine, West China Hospital, Sichuan University, Chengdu 610041, China; Corresponding authorDepartment of Neurosurgery, the First Affiliated Hospital of Jinan University, Guangzhou, China; Department of Neurosurgery, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, China; Laboratory of Neurological Diseases and Brain Function, the Affiliated Hospital, Southwest Medical University, Luzhou 646000, China; Corresponding authorSummary: Under pathological conditions, astrocytes can transfer mitochondria to neurons, where they exert neuroprotective effects. In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photon microscope. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. We then detail procedures for the preparation of a coverslip with a customized titanium ring and cranial window for two-photon microscopy scanning.For complete details on the use and execution of this protocol, please refer to Zhou et al. 1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000061Health SciencesMicroscopyNeuroscience |
| spellingShingle | Jian Zhou Fengling Du Fan Zhang Xianhui Zhang Yuanyuan Wu Lifang Zhang Ping Liao Tianqi Tu Jianhua Peng Tao Li Yong Jiang Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy STAR Protocols Health Sciences Microscopy Neuroscience |
| title | Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy |
| title_full | Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy |
| title_fullStr | Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy |
| title_full_unstemmed | Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy |
| title_short | Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy |
| title_sort | protocol for investigating astrocytic mitochondria in neurons of adult mice using two photon microscopy |
| topic | Health Sciences Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000061 |
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