CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE
As a CXC subtype member of the chemokine superfamily, CXCL13 is considered to be involved in systemic lupus erythematosus (SLE), especially in lupus nephritis (LN). To determine the effect of CXCL13 on SLE and explore the potential mechanisms, we tested serum concentrations of CXCL13 in patients and...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/2063985 |
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| author | Zhanyun Da Liuxia Li Jin Zhu Zhifeng Gu Bo You Ying Shan Si Shi |
| author_facet | Zhanyun Da Liuxia Li Jin Zhu Zhifeng Gu Bo You Ying Shan Si Shi |
| author_sort | Zhanyun Da |
| collection | DOAJ |
| description | As a CXC subtype member of the chemokine superfamily, CXCL13 is considered to be involved in systemic lupus erythematosus (SLE), especially in lupus nephritis (LN). To determine the effect of CXCL13 on SLE and explore the potential mechanisms, we tested serum concentrations of CXCL13 in patients and healthy individuals and found that CXCL13 expression was high in SLE patients especially in LN patients. When we treated human renal mesangial cells (HRMCs) in vitro with recombinant human CXCL13, the cell proliferation was accelerated, which was tested by Cell Counting Kit-8 assay and flow cytometry. Western blot and immunofluorescence assay revealed that CXCL13 would lead to phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). However, the effect was weakened after the silence of CXCR5. The results of our study elaborated that high expression of CXCL13 could be involved in the pathogenesis of LN. |
| format | Article |
| id | doaj-art-231adafbbd0141a0aa41c05e10c69f04 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-231adafbbd0141a0aa41c05e10c69f042025-02-03T05:52:16ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/20639852063985CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLEZhanyun Da0Liuxia Li1Jin Zhu2Zhifeng Gu3Bo You4Ying Shan5Si Shi6Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, ChinaDepartment of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, ChinaDepartment of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, ChinaDepartment of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, ChinaAs a CXC subtype member of the chemokine superfamily, CXCL13 is considered to be involved in systemic lupus erythematosus (SLE), especially in lupus nephritis (LN). To determine the effect of CXCL13 on SLE and explore the potential mechanisms, we tested serum concentrations of CXCL13 in patients and healthy individuals and found that CXCL13 expression was high in SLE patients especially in LN patients. When we treated human renal mesangial cells (HRMCs) in vitro with recombinant human CXCL13, the cell proliferation was accelerated, which was tested by Cell Counting Kit-8 assay and flow cytometry. Western blot and immunofluorescence assay revealed that CXCL13 would lead to phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). However, the effect was weakened after the silence of CXCR5. The results of our study elaborated that high expression of CXCL13 could be involved in the pathogenesis of LN.http://dx.doi.org/10.1155/2016/2063985 |
| spellingShingle | Zhanyun Da Liuxia Li Jin Zhu Zhifeng Gu Bo You Ying Shan Si Shi CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE Journal of Immunology Research |
| title | CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE |
| title_full | CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE |
| title_fullStr | CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE |
| title_full_unstemmed | CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE |
| title_short | CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE |
| title_sort | cxcl13 promotes proliferation of mesangial cells by combination with cxcr5 in sle |
| url | http://dx.doi.org/10.1155/2016/2063985 |
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