Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines
Abstract Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are transcribed in thymic epithelium, while immature T lymphocytes express functional neurohypophysial receptors. Neurohypophysial receptors belong to the G protein-linked seven-transmembrane receptor superfamily and are encoded...
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| Format: | Article |
| Language: | English |
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Wiley
2004-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/10446670410001670481 |
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| author | I. Hansenne G. Rasier Ch. Charlet-renard M.-P. Defresne R. Greimers C. Breton J.-J. Legros V. Geenen H. Martens |
| author_facet | I. Hansenne G. Rasier Ch. Charlet-renard M.-P. Defresne R. Greimers C. Breton J.-J. Legros V. Geenen H. Martens |
| author_sort | I. Hansenne |
| collection | DOAJ |
| description | Abstract
Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are
transcribed in thymic epithelium, while immature T lymphocytes express
functional neurohypophysial receptors. Neurohypophysial receptors belong
to the G protein-linked seven-transmembrane receptor superfamily and are encoded
by four distinct genes, OTR, V1R, V2R
and V3R. The objective of this study was to
identify the nature of neurohypophysial receptor in thymic T cell subsets purified by
immunomagnetic selection, as well as in murine thymic lymphoma cell lines RL12-NP
and BW5147. OTR is transcribed in all thymic T cell subsets and T cell lines, while
V3R transcription is restricted to CD4+ CD8+ and CD8+ thymic cells. Neither V1R nor
V2R transcripts are detected in any kind of T cells. The OTR protein was identified by
immunocytochemistry on thymocytes freshly isolated from C57BL/6 mice. In murine fetal
thymic organ cultures, a specific OTR antagonist does not modify the percentage of T
cell subsets, but increases late T cell apoptosis further evidencing the involvement of
OT/OTR signaling in the control of T cell proliferation and survival. According to these
data, OTR and V3R are differentially expressed during T cell ontogeny. Moreover, the
restriction of OTR transcription to T cell lines derived from thymic lymphomas may be
important in the context of T cell leukemia pathogenesis and treatment. |
| format | Article |
| id | doaj-art-23076bfaaedc42e0a6d0c440052406fb |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-23076bfaaedc42e0a6d0c440052406fb2025-02-03T01:02:28ZengWileyClinical and Developmental Immunology1740-25221740-25302004-01-01111455110.1080/10446670410001670481Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell LinesI. Hansenne0G. Rasier1Ch. Charlet-renard2M.-P. Defresne3R. Greimers4C. Breton5J.-J. Legros6V. Geenen7H. Martens8Center of Immunology, University of Liege, Institute of Pathology CHU-B23, Liege-Sart Tilman B-4000, BelgiumCenter of Immunology, University of Liege, Institute of Pathology CHU-B23, Liege-Sart Tilman B-4000, BelgiumCenter of Immunology, University of Liege, Institute of Pathology CHU-B23, Liege-Sart Tilman B-4000, BelgiumCenter of Cancerology, University of Liege, Institute of Pathology CHU-B23, Liege-Sart Tilman B-4000, BelgiumCenter of Cancerology, University of Liege, Institute of Pathology CHU-B23, Liege-Sart Tilman B-4000, BelgiumEndocrinology of Annelidae Laboratory, University of Lille I, Villeneuve d'Ascq F-59655, FranceCenter of Immunology, University of Liege, Institute of Pathology CHU-B23, Liege-Sart Tilman B-4000, BelgiumCenter of Immunology, University of Liege, Institute of Pathology CHU-B23, Liege-Sart Tilman B-4000, BelgiumCenter of Immunology, University of Liege, Institute of Pathology CHU-B23, Liege-Sart Tilman B-4000, BelgiumAbstract Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are transcribed in thymic epithelium, while immature T lymphocytes express functional neurohypophysial receptors. Neurohypophysial receptors belong to the G protein-linked seven-transmembrane receptor superfamily and are encoded by four distinct genes, OTR, V1R, V2R and V3R. The objective of this study was to identify the nature of neurohypophysial receptor in thymic T cell subsets purified by immunomagnetic selection, as well as in murine thymic lymphoma cell lines RL12-NP and BW5147. OTR is transcribed in all thymic T cell subsets and T cell lines, while V3R transcription is restricted to CD4+ CD8+ and CD8+ thymic cells. Neither V1R nor V2R transcripts are detected in any kind of T cells. The OTR protein was identified by immunocytochemistry on thymocytes freshly isolated from C57BL/6 mice. In murine fetal thymic organ cultures, a specific OTR antagonist does not modify the percentage of T cell subsets, but increases late T cell apoptosis further evidencing the involvement of OT/OTR signaling in the control of T cell proliferation and survival. According to these data, OTR and V3R are differentially expressed during T cell ontogeny. Moreover, the restriction of OTR transcription to T cell lines derived from thymic lymphomas may be important in the context of T cell leukemia pathogenesis and treatment.http://dx.doi.org/10.1080/10446670410001670481 |
| spellingShingle | I. Hansenne G. Rasier Ch. Charlet-renard M.-P. Defresne R. Greimers C. Breton J.-J. Legros V. Geenen H. Martens Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines Clinical and Developmental Immunology |
| title | Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines |
| title_full | Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines |
| title_fullStr | Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines |
| title_full_unstemmed | Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines |
| title_short | Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines |
| title_sort | neurohypophysial receptor gene expression by thymic t cell subsets and thymic t cell lymphoma cell lines |
| url | http://dx.doi.org/10.1080/10446670410001670481 |
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