Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines
Abstract Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are transcribed in thymic epithelium, while immature T lymphocytes express functional neurohypophysial receptors. Neurohypophysial receptors belong to the G protein-linked seven-transmembrane receptor superfamily and are encoded...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2004-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/10446670410001670481 |
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| Summary: | Abstract
Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are
transcribed in thymic epithelium, while immature T lymphocytes express
functional neurohypophysial receptors. Neurohypophysial receptors belong
to the G protein-linked seven-transmembrane receptor superfamily and are encoded
by four distinct genes, OTR, V1R, V2R
and V3R. The objective of this study was to
identify the nature of neurohypophysial receptor in thymic T cell subsets purified by
immunomagnetic selection, as well as in murine thymic lymphoma cell lines RL12-NP
and BW5147. OTR is transcribed in all thymic T cell subsets and T cell lines, while
V3R transcription is restricted to CD4+ CD8+ and CD8+ thymic cells. Neither V1R nor
V2R transcripts are detected in any kind of T cells. The OTR protein was identified by
immunocytochemistry on thymocytes freshly isolated from C57BL/6 mice. In murine fetal
thymic organ cultures, a specific OTR antagonist does not modify the percentage of T
cell subsets, but increases late T cell apoptosis further evidencing the involvement of
OT/OTR signaling in the control of T cell proliferation and survival. According to these
data, OTR and V3R are differentially expressed during T cell ontogeny. Moreover, the
restriction of OTR transcription to T cell lines derived from thymic lymphomas may be
important in the context of T cell leukemia pathogenesis and treatment. |
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| ISSN: | 1740-2522 1740-2530 |