The forkhead transcription factor Foxl2 is sumoylated in both human and mouse: sumoylation affects its stability, localization, and activity.

The FOXL2 forkhead transcription factor is expressed in ovarian granulosa cells, and mutated FOXL2 causes the blepharophimosis, ptosis and epicanthus inversus syndrome (BPES) and predisposes to premature ovarian failure. Inactivation of Foxl2 in mice demonstrated its indispensability for female gona...

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Main Authors: Mara Marongiu, Manila Deiana, Alessandra Meloni, Loredana Marcia, Alessandro Puddu, Antonio Cao, David Schlessinger, Laura Crisponi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-03-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009477&type=printable
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author Mara Marongiu
Manila Deiana
Alessandra Meloni
Loredana Marcia
Alessandro Puddu
Antonio Cao
David Schlessinger
Laura Crisponi
author_facet Mara Marongiu
Manila Deiana
Alessandra Meloni
Loredana Marcia
Alessandro Puddu
Antonio Cao
David Schlessinger
Laura Crisponi
author_sort Mara Marongiu
collection DOAJ
description The FOXL2 forkhead transcription factor is expressed in ovarian granulosa cells, and mutated FOXL2 causes the blepharophimosis, ptosis and epicanthus inversus syndrome (BPES) and predisposes to premature ovarian failure. Inactivation of Foxl2 in mice demonstrated its indispensability for female gonadal sex determination and ovary development and revealed its antagonism of Sox9, the effector of male testis development. To help to define the regulatory activities of FOXL2, we looked for interacting proteins. Based on yeast two-hybrid screening, we found that FOXL2 interacts with PIAS1 and UBC9, both parts of the sumoylation machinery. We showed that human FOXL2 is sumoylated in transfected cell lines, and that endogenous mouse Foxl2 is comparably sumoylated. This modification changes its cellular localization, stability and transcriptional activity. It is intriguing that similar sumoylation and regulatory consequences have also been reported for SOX9, the male counterpart of FOXL2 in somatic gonadal tissues.
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id doaj-art-22fe7f94ce1e4c5a803578b0c2273ccf
institution DOAJ
issn 1932-6203
language English
publishDate 2010-03-01
publisher Public Library of Science (PLoS)
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series PLoS ONE
spelling doaj-art-22fe7f94ce1e4c5a803578b0c2273ccf2025-08-20T03:07:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-03-0153e947710.1371/journal.pone.0009477The forkhead transcription factor Foxl2 is sumoylated in both human and mouse: sumoylation affects its stability, localization, and activity.Mara MarongiuManila DeianaAlessandra MeloniLoredana MarciaAlessandro PudduAntonio CaoDavid SchlessingerLaura CrisponiThe FOXL2 forkhead transcription factor is expressed in ovarian granulosa cells, and mutated FOXL2 causes the blepharophimosis, ptosis and epicanthus inversus syndrome (BPES) and predisposes to premature ovarian failure. Inactivation of Foxl2 in mice demonstrated its indispensability for female gonadal sex determination and ovary development and revealed its antagonism of Sox9, the effector of male testis development. To help to define the regulatory activities of FOXL2, we looked for interacting proteins. Based on yeast two-hybrid screening, we found that FOXL2 interacts with PIAS1 and UBC9, both parts of the sumoylation machinery. We showed that human FOXL2 is sumoylated in transfected cell lines, and that endogenous mouse Foxl2 is comparably sumoylated. This modification changes its cellular localization, stability and transcriptional activity. It is intriguing that similar sumoylation and regulatory consequences have also been reported for SOX9, the male counterpart of FOXL2 in somatic gonadal tissues.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009477&type=printable
spellingShingle Mara Marongiu
Manila Deiana
Alessandra Meloni
Loredana Marcia
Alessandro Puddu
Antonio Cao
David Schlessinger
Laura Crisponi
The forkhead transcription factor Foxl2 is sumoylated in both human and mouse: sumoylation affects its stability, localization, and activity.
PLoS ONE
title The forkhead transcription factor Foxl2 is sumoylated in both human and mouse: sumoylation affects its stability, localization, and activity.
title_full The forkhead transcription factor Foxl2 is sumoylated in both human and mouse: sumoylation affects its stability, localization, and activity.
title_fullStr The forkhead transcription factor Foxl2 is sumoylated in both human and mouse: sumoylation affects its stability, localization, and activity.
title_full_unstemmed The forkhead transcription factor Foxl2 is sumoylated in both human and mouse: sumoylation affects its stability, localization, and activity.
title_short The forkhead transcription factor Foxl2 is sumoylated in both human and mouse: sumoylation affects its stability, localization, and activity.
title_sort forkhead transcription factor foxl2 is sumoylated in both human and mouse sumoylation affects its stability localization and activity
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009477&type=printable
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