Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase
Ornithine aminotransferase (OAT) catalyzes transfer of the delta-amino group from L-ornithine to oxo-glutarate. In plants, this reaction biochemically connects urea cycle, proline cycle, and polyamine biosynthesis pathway. OAT activity is shown to be associated with biotic and abiotic stress respons...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2022-04-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/3291 |
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| author | A. A. Egorova S. V. Gerasimova A. V. Kochetov |
| author_facet | A. A. Egorova S. V. Gerasimova A. V. Kochetov |
| author_sort | A. A. Egorova |
| collection | DOAJ |
| description | Ornithine aminotransferase (OAT) catalyzes transfer of the delta-amino group from L-ornithine to oxo-glutarate. In plants, this reaction biochemically connects urea cycle, proline cycle, and polyamine biosynthesis pathway. OAT activity is shown to be associated with biotic and abiotic stress responses and nitrogen metabolism, but its physiological role is still unclear. In our study, we decided to investigate transcriptional regulation of the OAT gene in Arabidopsis thaliana under normal conditions and in response to various growth regulators. In the present work, the reporter gene construct containing the Escherichia coli β-glucuronidase gene (gus) under control of the A. thaliana OAT gene promoter was introduced into the genome of A. thaliana ecotype Columbia plants using the floral dip method; GUS activity was assayed in different experimental conditions including hormone treatment, low and high nitrogen and salinity. The GUS activity was analyzed histochemically. Plants were incubated with staining solution containing X-Gluc. We show that under standard growth conditions, the promoter is active during germination and in developing floral organs. OAT promoter activity specifically activates in response to different forms of auxin (IAA, NAA, and 2,4D), cytokinin (6- BAP), ethylene precursor (ACC), high nitrogen and salinity. Analysis of the OAT expression by qRT-PCR confirmed the pattern observed using the GUS reporter system. The OAT gene showed a significantly elevated expression in fourday- old seedlings and in plant roots in response to auxins and cytokinins. The analysis of the OAT promoter structure reveals cis-acting regulatory DNA elements associated with auxin regulation and abiotic stresses. The results of the study indicate that the OAT gene is involved in developmental processes and is regulated by auxin and cytokinins. |
| format | Article |
| id | doaj-art-22f8c6037fca46f5b5e0d3ce016dc453 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-22f8c6037fca46f5b5e0d3ce016dc4532025-02-01T09:58:11ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592022-04-0126215315810.18699/VJGB-22-191246Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferaseA. A. Egorova0S. V. Gerasimova1A. V. Kochetov2Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityOrnithine aminotransferase (OAT) catalyzes transfer of the delta-amino group from L-ornithine to oxo-glutarate. In plants, this reaction biochemically connects urea cycle, proline cycle, and polyamine biosynthesis pathway. OAT activity is shown to be associated with biotic and abiotic stress responses and nitrogen metabolism, but its physiological role is still unclear. In our study, we decided to investigate transcriptional regulation of the OAT gene in Arabidopsis thaliana under normal conditions and in response to various growth regulators. In the present work, the reporter gene construct containing the Escherichia coli β-glucuronidase gene (gus) under control of the A. thaliana OAT gene promoter was introduced into the genome of A. thaliana ecotype Columbia plants using the floral dip method; GUS activity was assayed in different experimental conditions including hormone treatment, low and high nitrogen and salinity. The GUS activity was analyzed histochemically. Plants were incubated with staining solution containing X-Gluc. We show that under standard growth conditions, the promoter is active during germination and in developing floral organs. OAT promoter activity specifically activates in response to different forms of auxin (IAA, NAA, and 2,4D), cytokinin (6- BAP), ethylene precursor (ACC), high nitrogen and salinity. Analysis of the OAT expression by qRT-PCR confirmed the pattern observed using the GUS reporter system. The OAT gene showed a significantly elevated expression in fourday- old seedlings and in plant roots in response to auxins and cytokinins. The analysis of the OAT promoter structure reveals cis-acting regulatory DNA elements associated with auxin regulation and abiotic stresses. The results of the study indicate that the OAT gene is involved in developmental processes and is regulated by auxin and cytokinins.https://vavilov.elpub.ru/jour/article/view/3291ornithine aminotransferasearabidopsis thalianaauxinnitrogendevelopment |
| spellingShingle | A. A. Egorova S. V. Gerasimova A. V. Kochetov Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase Вавиловский журнал генетики и селекции ornithine aminotransferase arabidopsis thaliana auxin nitrogen development |
| title | Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase |
| title_full | Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase |
| title_fullStr | Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase |
| title_full_unstemmed | Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase |
| title_short | Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase |
| title_sort | developmental and hormonal regulation of arabidopsis thaliana ornithine delta aminotransferase |
| topic | ornithine aminotransferase arabidopsis thaliana auxin nitrogen development |
| url | https://vavilov.elpub.ru/jour/article/view/3291 |
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