Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathway
IntroductionThis study investigates the potential of Bifidobacterium breve BB05 (BB05) in mitigating depressive symptoms in a mouse model of Chronic Unpredictable Mild Stress (CUMS), with fluoxetine as a positive control.Methods and ResultsHigh-dose BB05 (1.0 × 109 CFU/kg, BB05H) significantly reduc...
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| Format: | Article |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2025.1529566/full |
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| author | Yanni Pan Qingling Huang Yuan Liang Yuwuqi Xie Fang Tan Xingyao Long |
| author_facet | Yanni Pan Qingling Huang Yuan Liang Yuwuqi Xie Fang Tan Xingyao Long |
| author_sort | Yanni Pan |
| collection | DOAJ |
| description | IntroductionThis study investigates the potential of Bifidobacterium breve BB05 (BB05) in mitigating depressive symptoms in a mouse model of Chronic Unpredictable Mild Stress (CUMS), with fluoxetine as a positive control.Methods and ResultsHigh-dose BB05 (1.0 × 109 CFU/kg, BB05H) significantly reduced anxiety- and depression-like behaviors in CUMS mice, as measured by the open field test, tail suspension test, and forced swim test. BB05 treatment also reduced pathological ileal damage, alleviated inflammation, and lowered serum levels of pro-inflammatory cytokines IL-6 and TNF-α. Additionally, BB05 increased serum 5-HT levels and decreased ACTH concentrations. Mechanistic analysis revealed that BB05 exerts antidepressant effects by activating the AKT/mTOR signaling pathway in the prefrontal cortex, promoting neuroprotection, neurogenesis, and synaptic plasticity.DiscussionThese findings suggest that BB05, particularly at higher doses, effectively alleviates CUMS-induced depressive behaviors and improves physiological outcomes, supporting the use of probiotics as a potential treatment for depression by targeting the AKT/mTOR pathway. |
| format | Article |
| id | doaj-art-22e4a47db1004b02b8053fed48538c39 |
| institution | Kabale University |
| issn | 2296-861X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj-art-22e4a47db1004b02b8053fed48538c392025-01-30T05:10:07ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-01-011210.3389/fnut.2025.15295661529566Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathwayYanni Pan0Qingling Huang1Yuan Liang2Yuwuqi Xie3Fang Tan4Xingyao Long5Chongqing Engineering Laboratory for Research and Development of Functional Food, Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, ChinaDepartment of Sleep and Psychology, The Fifth People's Hospital of Chongqing, Chongqing, ChinaSchool of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, ChinaSchool of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, ChinaCollege of Pre-School, Chongqing University of Education, Chongqing, ChinaChongqing Engineering Laboratory for Research and Development of Functional Food, Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, ChinaIntroductionThis study investigates the potential of Bifidobacterium breve BB05 (BB05) in mitigating depressive symptoms in a mouse model of Chronic Unpredictable Mild Stress (CUMS), with fluoxetine as a positive control.Methods and ResultsHigh-dose BB05 (1.0 × 109 CFU/kg, BB05H) significantly reduced anxiety- and depression-like behaviors in CUMS mice, as measured by the open field test, tail suspension test, and forced swim test. BB05 treatment also reduced pathological ileal damage, alleviated inflammation, and lowered serum levels of pro-inflammatory cytokines IL-6 and TNF-α. Additionally, BB05 increased serum 5-HT levels and decreased ACTH concentrations. Mechanistic analysis revealed that BB05 exerts antidepressant effects by activating the AKT/mTOR signaling pathway in the prefrontal cortex, promoting neuroprotection, neurogenesis, and synaptic plasticity.DiscussionThese findings suggest that BB05, particularly at higher doses, effectively alleviates CUMS-induced depressive behaviors and improves physiological outcomes, supporting the use of probiotics as a potential treatment for depression by targeting the AKT/mTOR pathway.https://www.frontiersin.org/articles/10.3389/fnut.2025.1529566/fullBifidobacterium brevedepressive symptomsprobioticschronic unpredictable mild stressAKT/mTOR pathway |
| spellingShingle | Yanni Pan Qingling Huang Yuan Liang Yuwuqi Xie Fang Tan Xingyao Long Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathway Frontiers in Nutrition Bifidobacterium breve depressive symptoms probiotics chronic unpredictable mild stress AKT/mTOR pathway |
| title | Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathway |
| title_full | Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathway |
| title_fullStr | Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathway |
| title_full_unstemmed | Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathway |
| title_short | Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathway |
| title_sort | bifidobacterium breve bb05 alleviates depressive symptoms in mice via the akt mtor pathway |
| topic | Bifidobacterium breve depressive symptoms probiotics chronic unpredictable mild stress AKT/mTOR pathway |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2025.1529566/full |
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