Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational Study
Background/Objectives: Breast cancer (BC) is a heterogeneous disease with multifactorial origins, including environmental, genetic, and immunological factors. Inflammatory cytokines, such as alpha 1 antitrypsin (α1-AT), are increased in BC and affect physiological and pathological conditions. This s...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Diseases |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-9721/13/1/1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588678498615296 |
|---|---|
| author | Guadalupe Ávalos-Navarro Luis A. Bautista-Herrera Asbiel Felipe Garibaldi-Ríos Ramiro Ramírez-Patiño Marisol Gutiérrez-García Perla Briseño-Álvarez Luis Felipe Jave-Suárez Emmanuel Reyes-Uribe Martha Patricia Gallegos-Arreola |
| author_facet | Guadalupe Ávalos-Navarro Luis A. Bautista-Herrera Asbiel Felipe Garibaldi-Ríos Ramiro Ramírez-Patiño Marisol Gutiérrez-García Perla Briseño-Álvarez Luis Felipe Jave-Suárez Emmanuel Reyes-Uribe Martha Patricia Gallegos-Arreola |
| author_sort | Guadalupe Ávalos-Navarro |
| collection | DOAJ |
| description | Background/Objectives: Breast cancer (BC) is a heterogeneous disease with multifactorial origins, including environmental, genetic, and immunological factors. Inflammatory cytokines, such as alpha 1 antitrypsin (α1-AT), are increased in BC and affect physiological and pathological conditions. This study aimed to evaluate the serum levels of α1-AT and perform a computational analysis of <i>SERPINA1</i> in BC, as well as their association with molecular subtypes and clinical features. Methods: For the experimental analysis, we evaluated 255 women with BC and 53 healthy women (HW) in a cross-sectional study. Molecular subtypes were identified by immunohistochemistry and TNM was used for clinical staging. Soluble levels of α1-AT were quantified by ELISA. Computational analysis of <i>SERPINA1</i> expression was performed using GEPIA and cBioPortal. Results: α1-AT was increased in BC women versus HW (75.8 ng/mL vs. 532.2 ng/mL). Luminal A had higher concentration (547.5 ng/mL) than Triple Negative (TN) (484.1 ng/mL), but the levels were not associated with clinical stage. The computational analysis showed that <i>SERPINA1</i> is overexpressed in BC with differential expression among subtypes; its overexpression is associated with a better prognosis, longer disease-free survival, and overall survival. Conclusions: α1-AT levels are increased in women with BC women compared to HW. The Luminal A subtype shows higher soluble protein levels than the TN one. Furthermore, <i>SERPINA1</i> mRNA overexpression in BC is linked to a protective effect. |
| format | Article |
| id | doaj-art-22aed771a23c42479f1152d88c8dc293 |
| institution | Kabale University |
| issn | 2079-9721 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diseases |
| spelling | doaj-art-22aed771a23c42479f1152d88c8dc2932025-01-24T13:29:12ZengMDPI AGDiseases2079-97212024-12-01131110.3390/diseases13010001Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational StudyGuadalupe Ávalos-Navarro0Luis A. Bautista-Herrera1Asbiel Felipe Garibaldi-Ríos2Ramiro Ramírez-Patiño3Marisol Gutiérrez-García4Perla Briseño-Álvarez5Luis Felipe Jave-Suárez6Emmanuel Reyes-Uribe7Martha Patricia Gallegos-Arreola8Departamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega (CUCIÉNEGA), Universidad de Guadalajara, Av. Universidad 1115, Lindavista, Ocotlán 47820, Jalisco, MexicoDepartamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingenierías (CUCEI), Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, Olímpica, Guadalajara 44430, Jalisco, MexicoDoctorado en Genética Humana, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Guadalajara 44340, Jalisco, MexicoDepartamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega (CUCIÉNEGA), Universidad de Guadalajara, Av. Universidad 1115, Lindavista, Ocotlán 47820, Jalisco, MexicoLicenciatura en Químico Farmacéutico Biólogo, Centro Universitario de Ciencias Exactas e Ingenierías (CUCEI), Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, Olímpica, Guadalajara 44430, Jalisco, MexicoLicenciatura en Químico Farmacéutico Biólogo, Centro Universitario de la Ciénega (CUCIÉNEGA), Universidad de Guadalajara, Av. Universidad 1115, Lindavista, Ocotlán 47820, Jalisco, MexicoDivisión de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoDepartamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega (CUCIÉNEGA), Universidad de Guadalajara, Av. Universidad 1115, Lindavista, Ocotlán 47820, Jalisco, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Independencia Oriente, Guadalajara 44340, Jalisco, MexicoBackground/Objectives: Breast cancer (BC) is a heterogeneous disease with multifactorial origins, including environmental, genetic, and immunological factors. Inflammatory cytokines, such as alpha 1 antitrypsin (α1-AT), are increased in BC and affect physiological and pathological conditions. This study aimed to evaluate the serum levels of α1-AT and perform a computational analysis of <i>SERPINA1</i> in BC, as well as their association with molecular subtypes and clinical features. Methods: For the experimental analysis, we evaluated 255 women with BC and 53 healthy women (HW) in a cross-sectional study. Molecular subtypes were identified by immunohistochemistry and TNM was used for clinical staging. Soluble levels of α1-AT were quantified by ELISA. Computational analysis of <i>SERPINA1</i> expression was performed using GEPIA and cBioPortal. Results: α1-AT was increased in BC women versus HW (75.8 ng/mL vs. 532.2 ng/mL). Luminal A had higher concentration (547.5 ng/mL) than Triple Negative (TN) (484.1 ng/mL), but the levels were not associated with clinical stage. The computational analysis showed that <i>SERPINA1</i> is overexpressed in BC with differential expression among subtypes; its overexpression is associated with a better prognosis, longer disease-free survival, and overall survival. Conclusions: α1-AT levels are increased in women with BC women compared to HW. The Luminal A subtype shows higher soluble protein levels than the TN one. Furthermore, <i>SERPINA1</i> mRNA overexpression in BC is linked to a protective effect.https://www.mdpi.com/2079-9721/13/1/1breast cancermolecular subtypeclinical stagesoluble levelsalpha1 antitrypsin<i>SERPINA1</i> |
| spellingShingle | Guadalupe Ávalos-Navarro Luis A. Bautista-Herrera Asbiel Felipe Garibaldi-Ríos Ramiro Ramírez-Patiño Marisol Gutiérrez-García Perla Briseño-Álvarez Luis Felipe Jave-Suárez Emmanuel Reyes-Uribe Martha Patricia Gallegos-Arreola Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational Study Diseases breast cancer molecular subtype clinical stage soluble levels alpha1 antitrypsin <i>SERPINA1</i> |
| title | Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational Study |
| title_full | Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational Study |
| title_fullStr | Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational Study |
| title_full_unstemmed | Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational Study |
| title_short | Serum α1-AT Levels and <i>SERPINA1</i> Molecular Analysis in Breast Cancer: An Experimental and Computational Study |
| title_sort | serum α1 at levels and i serpina1 i molecular analysis in breast cancer an experimental and computational study |
| topic | breast cancer molecular subtype clinical stage soluble levels alpha1 antitrypsin <i>SERPINA1</i> |
| url | https://www.mdpi.com/2079-9721/13/1/1 |
| work_keys_str_mv | AT guadalupeavalosnavarro seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy AT luisabautistaherrera seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy AT asbielfelipegaribaldirios seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy AT ramiroramirezpatino seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy AT marisolgutierrezgarcia seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy AT perlabrisenoalvarez seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy AT luisfelipejavesuarez seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy AT emmanuelreyesuribe seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy AT marthapatriciagallegosarreola seruma1atlevelsandiserpina1imolecularanalysisinbreastcanceranexperimentalandcomputationalstudy |