Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot
Tan spot caused by the fungus Pyrenophora tritici-repentis is an important leaf spot disease in wheat growing areas throughout the world. The study aims to identify wheat germplasm resistant to tan spot based on phytopathological screening and molecular marker analysis. A collection of 64 common whe...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-11-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2340 |
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| author | A. M. Kokhmetova M. N. Atishova M. T. Kumarbayeva I. N. Leonova |
| author_facet | A. M. Kokhmetova M. N. Atishova M. T. Kumarbayeva I. N. Leonova |
| author_sort | A. M. Kokhmetova |
| collection | DOAJ |
| description | Tan spot caused by the fungus Pyrenophora tritici-repentis is an important leaf spot disease in wheat growing areas throughout the world. The study aims to identify wheat germplasm resistant to tan spot based on phytopathological screening and molecular marker analysis. A collection of 64 common wheat germplasms, including cultivars and breeding lines from Kazakhstan and CIMMYT, was assessed for tan spot resistance in greenhouse conditions and characterized using the Xfcp623 molecular marker, diagnostic for the Tsn1 gene. All wheat cultivars/lines varied in their reaction to tan spot isolate race 1, ranging from susceptible to resistant. Most accessions studied (53 %) were susceptible to Ptr race 1. Spring wheat cultivars were more susceptible to race 1 than winter wheat cultivars. As a result of genotyping, an insensitive reaction to Ptr ToxA was predicted in 41 wheat cultivars (64 %). The tsn1 gene carriers identified included 27 Kazakhstani and 14 CIMMYT cultivars/lines, demonstrating insensitivity to Ptr ToxA. The majority of the Tsn1 genotype were sensitive to race 1 and showed susceptibility to the pathogen in the field. Disease scores from seedling stage positively correlated with field disease ratings. Of particular interest are 27 wheat accessions that demonstrated resistance to spore inoculation by Ptr race 1, were characterized by insensitivity to ToxA and showed field resistance to the pathogen. The results of this study will contribute to wheat breeding programs for tan spot resistance with Marker Assisted Selection using the closely flanking markers. |
| format | Article |
| id | doaj-art-226e77416faa4785a2e8fff48f481d34 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2019-11-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-226e77416faa4785a2e8fff48f481d342025-02-01T09:58:08ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-11-0123787988610.18699/VJ19.562985Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spotA. M. Kokhmetova0M. N. Atishova1M. T. Kumarbayeva2I. N. Leonova3Institute of Plant Biology and Biotechnology; Kazakh National Agrarian UniversityInstitute of Plant Biology and BiotechnologyInstitute of Plant Biology and Biotechnology; Kazakh National Agrarian UniversityInstitute of Cytology and Genetics, SB RASTan spot caused by the fungus Pyrenophora tritici-repentis is an important leaf spot disease in wheat growing areas throughout the world. The study aims to identify wheat germplasm resistant to tan spot based on phytopathological screening and molecular marker analysis. A collection of 64 common wheat germplasms, including cultivars and breeding lines from Kazakhstan and CIMMYT, was assessed for tan spot resistance in greenhouse conditions and characterized using the Xfcp623 molecular marker, diagnostic for the Tsn1 gene. All wheat cultivars/lines varied in their reaction to tan spot isolate race 1, ranging from susceptible to resistant. Most accessions studied (53 %) were susceptible to Ptr race 1. Spring wheat cultivars were more susceptible to race 1 than winter wheat cultivars. As a result of genotyping, an insensitive reaction to Ptr ToxA was predicted in 41 wheat cultivars (64 %). The tsn1 gene carriers identified included 27 Kazakhstani and 14 CIMMYT cultivars/lines, demonstrating insensitivity to Ptr ToxA. The majority of the Tsn1 genotype were sensitive to race 1 and showed susceptibility to the pathogen in the field. Disease scores from seedling stage positively correlated with field disease ratings. Of particular interest are 27 wheat accessions that demonstrated resistance to spore inoculation by Ptr race 1, were characterized by insensitivity to ToxA and showed field resistance to the pathogen. The results of this study will contribute to wheat breeding programs for tan spot resistance with Marker Assisted Selection using the closely flanking markers.https://vavilov.elpub.ru/jour/article/view/2340wheatmolecular markerspyrenophora tritici-repentistan spottsn1toxa |
| spellingShingle | A. M. Kokhmetova M. N. Atishova M. T. Kumarbayeva I. N. Leonova Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot Вавиловский журнал генетики и селекции wheat molecular markers pyrenophora tritici-repentis tan spot tsn1 toxa |
| title | Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot |
| title_full | Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot |
| title_fullStr | Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot |
| title_full_unstemmed | Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot |
| title_short | Phytopathological screening and molecular marker analysis of wheat germplasm from Kazakhstan and CIMMYT for resistance to tan spot |
| title_sort | phytopathological screening and molecular marker analysis of wheat germplasm from kazakhstan and cimmyt for resistance to tan spot |
| topic | wheat molecular markers pyrenophora tritici-repentis tan spot tsn1 toxa |
| url | https://vavilov.elpub.ru/jour/article/view/2340 |
| work_keys_str_mv | AT amkokhmetova phytopathologicalscreeningandmolecularmarkeranalysisofwheatgermplasmfromkazakhstanandcimmytforresistancetotanspot AT mnatishova phytopathologicalscreeningandmolecularmarkeranalysisofwheatgermplasmfromkazakhstanandcimmytforresistancetotanspot AT mtkumarbayeva phytopathologicalscreeningandmolecularmarkeranalysisofwheatgermplasmfromkazakhstanandcimmytforresistancetotanspot AT inleonova phytopathologicalscreeningandmolecularmarkeranalysisofwheatgermplasmfromkazakhstanandcimmytforresistancetotanspot |