Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods
The excessive use and ingestion of residues of sulfonamides harm the human body and the environment. To meet the requirements of the maximum residue limit specified by the Ministry of Agriculture of China, a rapid detection method urgently needs to be developed. In the present study, a molecularly i...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Advances in Polymer Technology |
| Online Access: | http://dx.doi.org/10.1155/2020/7647580 |
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| _version_ | 1832553245420027904 |
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| author | Jingbo He Li Zhang Longhua Xu Feifan Kong Zhixiang Xu |
| author_facet | Jingbo He Li Zhang Longhua Xu Feifan Kong Zhixiang Xu |
| author_sort | Jingbo He |
| collection | DOAJ |
| description | The excessive use and ingestion of residues of sulfonamides harm the human body and the environment. To meet the requirements of the maximum residue limit specified by the Ministry of Agriculture of China, a rapid detection method urgently needs to be developed. In the present study, a molecularly imprinted polymer (MIP) that could selectively recognize sulfadiazine (SDZ) was prepared using SDZ as the template molecule and methacrylic acid as the functional monomer. Using MIP as biomimetic antibody and Au@Pt@SiO2 nanozyme as a marker, a new biomimetic immunoassay was developed to detect sulfadiazine. Under the optimal conditions, the limit of detection (IC15) and sensitivity (IC50) of this method were 0.09 and 6.1 mg/L, respectively. To determine the accuracy of this method, honey and milk samples spiked with sulfadiazine were analyzed, with recoveries in the range of 70.8%-90.2%. The method was also used for the quantitative analysis of sulfadiazine residues in milk powder and milk samples, producing results which correlated well with those obtained by high-performance liquid chromatography. |
| format | Article |
| id | doaj-art-2261adc9c2b145e1a0d132f36ef72dc9 |
| institution | Kabale University |
| issn | 0730-6679 1098-2329 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Polymer Technology |
| spelling | doaj-art-2261adc9c2b145e1a0d132f36ef72dc92025-02-03T05:54:27ZengWileyAdvances in Polymer Technology0730-66791098-23292020-01-01202010.1155/2020/76475807647580Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in FoodsJingbo He0Li Zhang1Longhua Xu2Feifan Kong3Zhixiang Xu4Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaKey Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaKey Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaShandong Wuzhou Testing Co., Ltd., Sishui 273200, ChinaKey Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaThe excessive use and ingestion of residues of sulfonamides harm the human body and the environment. To meet the requirements of the maximum residue limit specified by the Ministry of Agriculture of China, a rapid detection method urgently needs to be developed. In the present study, a molecularly imprinted polymer (MIP) that could selectively recognize sulfadiazine (SDZ) was prepared using SDZ as the template molecule and methacrylic acid as the functional monomer. Using MIP as biomimetic antibody and Au@Pt@SiO2 nanozyme as a marker, a new biomimetic immunoassay was developed to detect sulfadiazine. Under the optimal conditions, the limit of detection (IC15) and sensitivity (IC50) of this method were 0.09 and 6.1 mg/L, respectively. To determine the accuracy of this method, honey and milk samples spiked with sulfadiazine were analyzed, with recoveries in the range of 70.8%-90.2%. The method was also used for the quantitative analysis of sulfadiazine residues in milk powder and milk samples, producing results which correlated well with those obtained by high-performance liquid chromatography.http://dx.doi.org/10.1155/2020/7647580 |
| spellingShingle | Jingbo He Li Zhang Longhua Xu Feifan Kong Zhixiang Xu Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods Advances in Polymer Technology |
| title | Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods |
| title_full | Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods |
| title_fullStr | Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods |
| title_full_unstemmed | Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods |
| title_short | Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods |
| title_sort | development of nanozyme labeled biomimetic immunoassay for determination of sulfadiazine residue in foods |
| url | http://dx.doi.org/10.1155/2020/7647580 |
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