Triiodothyronine (T3) increases the expression of the amphiregulin (AREG) oncogene by activating extranuclear pathways in MCF-7 breast cancer cells

Triiodothyronine (T3) increases the expression of the amphiregulin (AREG) oncogene by activating extranuclear pathways in MCF-7 breast cancer cells

ABSTRACT Objective: Considering that the αvβ3 integrin plays an important role in tumor metastasis, this study investigated the involvement of these pathways in mediating the triiodothyronine (T3) effects on amphiregulin (AREG) expression. Materials and methods: We treated MCF-7 cells with T3 (10...

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Main Authors: Maria Teresa De Sibio, Fernanda Cristina Fontes Moretto, Regiane Marques Castro Olimpio, Miriane de Oliveira, Lucas Solla Mathias, Vinícius Vigliazzi Peghinelli, Helena Paim Tilli, Bianca Mariani Gonçalves, Dariane Beatriz Marino Cardoso, Larissa Silva Dall Aqua, Igor de Carvalho Depra, Mariana Menezes Lourenço, Aline Carbonera Luvizon, Paula de Oliveira Montandon Hokama, Maria Tereza Nunes, Marna Eliana Sakalem, Célia Regina Nogueira
Format: Article
Language:English
Published: Brazilian Society of Endocrinology and Metabolism 2025-01-01
Series:Archives of Endocrinology and Metabolism
Subjects:
Triiodothyronine
breast adenocarcinoma
MCF-7 cells
AREG
αvβ3 integrin
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000305&lng=en&tlng=en
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Summary:ABSTRACT Objective: Considering that the αvβ3 integrin plays an important role in tumor metastasis, this study investigated the involvement of these pathways in mediating the triiodothyronine (T3) effects on amphiregulin (AREG) expression. Materials and methods: We treated MCF-7 cells with T3 (10 nM) for 1 hour in the presence or absence of inhibitors for αvβ3 integrin (RGD peptide), MAPK (PD98059), PI3K (LY294002), and protein synthesis (cycloheximide [CHX]). A control group (C) received no T3 or inhibitors. Analyses of mRNA and protein expression were done using RT-qPCR and Western blot, respectively. Results: We observed that T3 increased AREG expression, an effect that was suppressed by all inhibitors. This finding indicates that the activation of the αvβ3 integrin signaling pathway, via PI3K, MAPK/ERK, is necessary for the T3-mediated effects on AREG expression and highlights the involvement of nongenomic mechanisms. In addition, CHX completely abolished T3-induced AREG mRNA expression, indicating that this effect requires prior protein synthesis. Conclusion: The identification that T3 acts through this signaling pathway holds considerable potential for clinical application, as it could lead to the development of specific drugs to block it.
ISSN:2359-4292

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