RhoA Regulation of Cardiomyocyte Differentiation
Earlier findings from our laboratory implicated RhoA in heart developmental processes. To investigate factors that potentially regulate RhoA expression, RhoA gene organisation and promoter activity were analysed. Comparative analysis indicated strict conservation of both gene organisation and coding...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/491546 |
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| _version_ | 1832551863243767808 |
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| author | Mari Kaarbø Denis I. Crane Wayne G. Murrell |
| author_facet | Mari Kaarbø Denis I. Crane Wayne G. Murrell |
| author_sort | Mari Kaarbø |
| collection | DOAJ |
| description | Earlier findings from our laboratory implicated RhoA in heart developmental processes. To investigate factors that potentially regulate RhoA expression, RhoA gene organisation and promoter activity were analysed. Comparative analysis indicated strict conservation of both gene organisation and coding sequence of the chick, mouse, and human RhoA genes. Bioinformatics analysis of the derived promoter region of mouse RhoA identified putative consensus sequence binding sites for several transcription factors involved in heart formation and organogenesis generally. Using luciferase reporter assays, RhoA promoter activity was shown to increase in mouse-derived P19CL6 cells that were induced to differentiate into cardiomyocytes. Overexpression of a dominant negative mutant of mouse RhoA (mRhoAN19) blocked this cardiomyocyte differentiation of P19CL6 cells and led to the accumulation of the cardiac transcription factors SRF and GATA4 and the early cardiac marker cardiac α-actin. Taken together, these findings indicate a fundamental role for RhoA in the differentiation of cardiomyocytes. |
| format | Article |
| id | doaj-art-2256c55771594abeb4ee03a2364f433b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2256c55771594abeb4ee03a2364f433b2025-02-03T06:00:19ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/491546491546RhoA Regulation of Cardiomyocyte DifferentiationMari Kaarbø0Denis I. Crane1Wayne G. Murrell2Department of Microbiology, University of Oslo, Oslo University Hospital, Rikshospitalet, 0454 Oslo, NorwayCell Biology, Eskitis Institute for Cell and Molecular Therapies, and School of Biomolecular and Biomedical Science, Griffith University, Nathan, QD 4111, AustraliaVilhelm Magnus Laboratory for Neurosurgical Research, Institute for Surgical Research, Oslo University Hospital, Rikshospitalet, 0454 Oslo, NorwayEarlier findings from our laboratory implicated RhoA in heart developmental processes. To investigate factors that potentially regulate RhoA expression, RhoA gene organisation and promoter activity were analysed. Comparative analysis indicated strict conservation of both gene organisation and coding sequence of the chick, mouse, and human RhoA genes. Bioinformatics analysis of the derived promoter region of mouse RhoA identified putative consensus sequence binding sites for several transcription factors involved in heart formation and organogenesis generally. Using luciferase reporter assays, RhoA promoter activity was shown to increase in mouse-derived P19CL6 cells that were induced to differentiate into cardiomyocytes. Overexpression of a dominant negative mutant of mouse RhoA (mRhoAN19) blocked this cardiomyocyte differentiation of P19CL6 cells and led to the accumulation of the cardiac transcription factors SRF and GATA4 and the early cardiac marker cardiac α-actin. Taken together, these findings indicate a fundamental role for RhoA in the differentiation of cardiomyocytes.http://dx.doi.org/10.1155/2013/491546 |
| spellingShingle | Mari Kaarbø Denis I. Crane Wayne G. Murrell RhoA Regulation of Cardiomyocyte Differentiation The Scientific World Journal |
| title | RhoA Regulation of Cardiomyocyte Differentiation |
| title_full | RhoA Regulation of Cardiomyocyte Differentiation |
| title_fullStr | RhoA Regulation of Cardiomyocyte Differentiation |
| title_full_unstemmed | RhoA Regulation of Cardiomyocyte Differentiation |
| title_short | RhoA Regulation of Cardiomyocyte Differentiation |
| title_sort | rhoa regulation of cardiomyocyte differentiation |
| url | http://dx.doi.org/10.1155/2013/491546 |
| work_keys_str_mv | AT marikaarbø rhoaregulationofcardiomyocytedifferentiation AT denisicrane rhoaregulationofcardiomyocytedifferentiation AT waynegmurrell rhoaregulationofcardiomyocytedifferentiation |