STUDIES OF THE BHK-21/2-17 LINEAGE USING FLOW CYTOMETRY
The paper presents results of the ВНК-21/2-17 lineage cell cycle tests using flow cytometry. This cell line is widely used in biological industry that is why studying the ВНК-21/2-17 cell suspension pattern using the proposed technique and determination of tools and methods to prevent morphological...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/334 |
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| author | M. A. Shevchenko M. N. Guseva D. V. Mikhalishin B. L. Manin A. A. Shishkova |
| author_facet | M. A. Shevchenko M. N. Guseva D. V. Mikhalishin B. L. Manin A. A. Shishkova |
| author_sort | M. A. Shevchenko |
| collection | DOAJ |
| description | The paper presents results of the ВНК-21/2-17 lineage cell cycle tests using flow cytometry. This cell line is widely used in biological industry that is why studying the ВНК-21/2-17 cell suspension pattern using the proposed technique and determination of tools and methods to prevent morphological and structural cell modifications in the culture affected by negative factors is of great interest. Flow cytometry allows to determine quantitative parameters of cell cycle phases and perform correlation between these phases and viability of the working cell population. It was mentioned that the general trend of this cell lineage suspension cultivation for 48 hours has a standard dynamics, herewith at all stages of the cell cycle the primary growth stage - G1 prevailed. Under unfavorable conditions of cultivation apoptotic debris could increase up to 31-59%, i. e. part of the cell population could be potentially non-reproductive. Unsuccessful cultivation stages associated with low pH content in the cultivator where pH was not maintained at the constant level were detected. During optimization of cell cultivation physical and chemical parameters proliferation intensity increased. Viable cell yield during cultivation when a specific pH was maintained was higher and at the synthetic stage G2 + M no cycle delay occurred. |
| format | Article |
| id | doaj-art-22457c660c854b5bb028cc1328ef3f04 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-22457c660c854b5bb028cc1328ef3f042025-02-06T09:52:06ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01045862334STUDIES OF THE BHK-21/2-17 LINEAGE USING FLOW CYTOMETRYM. A. Shevchenko0M. N. Guseva1D. V. Mikhalishin2B. L. Manin3A. A. Shishkova4FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThe paper presents results of the ВНК-21/2-17 lineage cell cycle tests using flow cytometry. This cell line is widely used in biological industry that is why studying the ВНК-21/2-17 cell suspension pattern using the proposed technique and determination of tools and methods to prevent morphological and structural cell modifications in the culture affected by negative factors is of great interest. Flow cytometry allows to determine quantitative parameters of cell cycle phases and perform correlation between these phases and viability of the working cell population. It was mentioned that the general trend of this cell lineage suspension cultivation for 48 hours has a standard dynamics, herewith at all stages of the cell cycle the primary growth stage - G1 prevailed. Under unfavorable conditions of cultivation apoptotic debris could increase up to 31-59%, i. e. part of the cell population could be potentially non-reproductive. Unsuccessful cultivation stages associated with low pH content in the cultivator where pH was not maintained at the constant level were detected. During optimization of cell cultivation physical and chemical parameters proliferation intensity increased. Viable cell yield during cultivation when a specific pH was maintained was higher and at the synthetic stage G2 + M no cycle delay occurred.https://veterinary.arriah.ru/jour/article/view/334проточная цитометрияапоптозфазы клеточного циклаflow cytometryapoptosiscell cycle phases |
| spellingShingle | M. A. Shevchenko M. N. Guseva D. V. Mikhalishin B. L. Manin A. A. Shishkova STUDIES OF THE BHK-21/2-17 LINEAGE USING FLOW CYTOMETRY Ветеринария сегодня проточная цитометрия апоптоз фазы клеточного цикла flow cytometry apoptosis cell cycle phases |
| title | STUDIES OF THE BHK-21/2-17 LINEAGE USING FLOW CYTOMETRY |
| title_full | STUDIES OF THE BHK-21/2-17 LINEAGE USING FLOW CYTOMETRY |
| title_fullStr | STUDIES OF THE BHK-21/2-17 LINEAGE USING FLOW CYTOMETRY |
| title_full_unstemmed | STUDIES OF THE BHK-21/2-17 LINEAGE USING FLOW CYTOMETRY |
| title_short | STUDIES OF THE BHK-21/2-17 LINEAGE USING FLOW CYTOMETRY |
| title_sort | studies of the bhk 21 2 17 lineage using flow cytometry |
| topic | проточная цитометрия апоптоз фазы клеточного цикла flow cytometry apoptosis cell cycle phases |
| url | https://veterinary.arriah.ru/jour/article/view/334 |
| work_keys_str_mv | AT mashevchenko studiesofthebhk21217lineageusingflowcytometry AT mnguseva studiesofthebhk21217lineageusingflowcytometry AT dvmikhalishin studiesofthebhk21217lineageusingflowcytometry AT blmanin studiesofthebhk21217lineageusingflowcytometry AT aashishkova studiesofthebhk21217lineageusingflowcytometry |