Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of <i>Acinetobacter baumannii</i> Clinical Strains
Background/Objectives: <i>Acinetobacter baumannii</i>, one of the most dangerous pathogens, is able to form biofilm structures and aggravate its treatment. For that reason, new antibiofilm agents are in need, and new sources of antibiofilm compounds are being sought from plants and their...
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2025-01-01
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| author | Tea Ganić Ilinka Pećinar Biljana Nikolić Dušan Kekić Nina Tomić Stefana Cvetković Stefana Vuletić Dragana Mitić-Ćulafić |
| author_facet | Tea Ganić Ilinka Pećinar Biljana Nikolić Dušan Kekić Nina Tomić Stefana Cvetković Stefana Vuletić Dragana Mitić-Ćulafić |
| author_sort | Tea Ganić |
| collection | DOAJ |
| description | Background/Objectives: <i>Acinetobacter baumannii</i>, one of the most dangerous pathogens, is able to form biofilm structures and aggravate its treatment. For that reason, new antibiofilm agents are in need, and new sources of antibiofilm compounds are being sought from plants and their products. Cinnamon essential oil is associated with a wide spectrum of biological activities, but with a further improvement of its physicochemical properties it could provide even better bioavailability. The aim of this work was the evaluation of the antibiofilm properties of cinnamon essential oil and its emulsion. Methods: In order to evaluate the antibiofilm activity, crystal violet assay was performed to determine biofilm biomass. The main components of the biofilm matrix were measured as well as the motile capacity of the tested strains. Gene expression was monitored with RT-qPCR, while treated biofilms were observed with Raman spectroscopy. Results: A particularly strong potential against pre-formed biofilm with a decreased biomass of up to 66% was found. The effect was monitored not only with regard to the whole biofilm biomass, but also on the individual components of the biofilm matrix such as exopolysaccharides, proteins, and eDNA molecules. Protein share drops in treated biofilms demonstrated the most consistency among strains and rose to 75%. The changes in strain motility and gene expressions were investigated after the treatments were carried out. Raman spectroscopy revealed the influence of the studied compounds on chemical bond types and the components present in the biofilm matrix of the tested strains. Conclusions: The results obtained from this research are promising regarding cinnamon essential oil and its emulsion as potential antibiofilm agents, so further investigation of their activity is encouraged for their potential use in biomedical applications. |
| format | Article |
| id | doaj-art-221ad6c4484c434ba3e16584cfc24d67 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-221ad6c4484c434ba3e16584cfc24d672025-01-24T13:19:02ZengMDPI AGAntibiotics2079-63822025-01-0114110610.3390/antibiotics14010106Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of <i>Acinetobacter baumannii</i> Clinical StrainsTea Ganić0Ilinka Pećinar1Biljana Nikolić2Dušan Kekić3Nina Tomić4Stefana Cvetković5Stefana Vuletić6Dragana Mitić-Ćulafić7Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaFaculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaFaculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade, 11000 Belgrade, SerbiaGroup for Biomedical Engineering and Nanobiotechnology, Institute of Technical Science of SASA, Kneza Mihaila 35/IV, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaBackground/Objectives: <i>Acinetobacter baumannii</i>, one of the most dangerous pathogens, is able to form biofilm structures and aggravate its treatment. For that reason, new antibiofilm agents are in need, and new sources of antibiofilm compounds are being sought from plants and their products. Cinnamon essential oil is associated with a wide spectrum of biological activities, but with a further improvement of its physicochemical properties it could provide even better bioavailability. The aim of this work was the evaluation of the antibiofilm properties of cinnamon essential oil and its emulsion. Methods: In order to evaluate the antibiofilm activity, crystal violet assay was performed to determine biofilm biomass. The main components of the biofilm matrix were measured as well as the motile capacity of the tested strains. Gene expression was monitored with RT-qPCR, while treated biofilms were observed with Raman spectroscopy. Results: A particularly strong potential against pre-formed biofilm with a decreased biomass of up to 66% was found. The effect was monitored not only with regard to the whole biofilm biomass, but also on the individual components of the biofilm matrix such as exopolysaccharides, proteins, and eDNA molecules. Protein share drops in treated biofilms demonstrated the most consistency among strains and rose to 75%. The changes in strain motility and gene expressions were investigated after the treatments were carried out. Raman spectroscopy revealed the influence of the studied compounds on chemical bond types and the components present in the biofilm matrix of the tested strains. Conclusions: The results obtained from this research are promising regarding cinnamon essential oil and its emulsion as potential antibiofilm agents, so further investigation of their activity is encouraged for their potential use in biomedical applications.https://www.mdpi.com/2079-6382/14/1/106cinnamon essential oilemulsion<i>Acinetobacter baumannii</i>biofilmRaman spectroscopygene expression |
| spellingShingle | Tea Ganić Ilinka Pećinar Biljana Nikolić Dušan Kekić Nina Tomić Stefana Cvetković Stefana Vuletić Dragana Mitić-Ćulafić Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of <i>Acinetobacter baumannii</i> Clinical Strains Antibiotics cinnamon essential oil emulsion <i>Acinetobacter baumannii</i> biofilm Raman spectroscopy gene expression |
| title | Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of <i>Acinetobacter baumannii</i> Clinical Strains |
| title_full | Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of <i>Acinetobacter baumannii</i> Clinical Strains |
| title_fullStr | Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of <i>Acinetobacter baumannii</i> Clinical Strains |
| title_full_unstemmed | Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of <i>Acinetobacter baumannii</i> Clinical Strains |
| title_short | Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of <i>Acinetobacter baumannii</i> Clinical Strains |
| title_sort | evaluation of cinnamon essential oil and its emulsion on biofilm associated components of i acinetobacter baumannii i clinical strains |
| topic | cinnamon essential oil emulsion <i>Acinetobacter baumannii</i> biofilm Raman spectroscopy gene expression |
| url | https://www.mdpi.com/2079-6382/14/1/106 |
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