Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed Extract
Aframomum melegueta Schum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract of A. melegueta. The antioxidant effects were evaluated using in vitro, 2, 2-dipheny...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2014/159343 |
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| author | Samuel Okwudili Onoja Yusuf Ndukaku Omeh Maxwell Ikechukwu Ezeja Martins Ndubuisi Chukwu |
| author_facet | Samuel Okwudili Onoja Yusuf Ndukaku Omeh Maxwell Ikechukwu Ezeja Martins Ndubuisi Chukwu |
| author_sort | Samuel Okwudili Onoja |
| collection | DOAJ |
| description | Aframomum melegueta Schum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract of A. melegueta. The antioxidant effects were evaluated using in vitro, 2, 2-diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase and thiobarbituric acid reactive substance assay method. The extract (25–400 μg/mL concentration) produced concentration dependent increase in antioxidant activity in 2, 2-diphenylpicrylhydrazine photometric assay. The extract (400 mg/kg) showed a significant (P<0.05) increase in serum catalase and superoxide dismutase activity when compared with the control group. The extract (400 mg/kg) showed a significant (P<0.05) decrease in the serum level of thiobarbituric acid reactive substance when compared with the control group. These findings suggest that the seed of A. melegueta has potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement. |
| format | Article |
| id | doaj-art-2210b3a24db04489a703a15884d52b85 |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-2210b3a24db04489a703a15884d52b852025-02-03T06:10:54ZengWileyJournal of Tropical Medicine1687-96861687-96942014-01-01201410.1155/2014/159343159343Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed ExtractSamuel Okwudili Onoja0Yusuf Ndukaku Omeh1Maxwell Ikechukwu Ezeja2Martins Ndubuisi Chukwu3Department of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, PMB 7267, Umudike, NigeriaDepartment of Biochemistry, College of Natural Sciences, Micheal Okpara University of Agriculture, PMB 7267, Umudike, NigeriaDepartment of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, PMB 7267, Umudike, NigeriaDepartment of Biochemistry, College of Natural Sciences, Micheal Okpara University of Agriculture, PMB 7267, Umudike, NigeriaAframomum melegueta Schum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract of A. melegueta. The antioxidant effects were evaluated using in vitro, 2, 2-diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase and thiobarbituric acid reactive substance assay method. The extract (25–400 μg/mL concentration) produced concentration dependent increase in antioxidant activity in 2, 2-diphenylpicrylhydrazine photometric assay. The extract (400 mg/kg) showed a significant (P<0.05) increase in serum catalase and superoxide dismutase activity when compared with the control group. The extract (400 mg/kg) showed a significant (P<0.05) decrease in the serum level of thiobarbituric acid reactive substance when compared with the control group. These findings suggest that the seed of A. melegueta has potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.http://dx.doi.org/10.1155/2014/159343 |
| spellingShingle | Samuel Okwudili Onoja Yusuf Ndukaku Omeh Maxwell Ikechukwu Ezeja Martins Ndubuisi Chukwu Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed Extract Journal of Tropical Medicine |
| title | Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed Extract |
| title_full | Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed Extract |
| title_fullStr | Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed Extract |
| title_full_unstemmed | Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed Extract |
| title_short | Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed Extract |
| title_sort | evaluation of the in vitro and in vivo antioxidant potentials of aframomum melegueta methanolic seed extract |
| url | http://dx.doi.org/10.1155/2014/159343 |
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