Multispectral Bioluminescence Tomography: Methodology and Simulation
Bioluminescent imaging has proven to be a valuable tool for monitoring physiological and pathological activities at cellular and molecular levels in living small animals. Using biological techniques, target cells can be tagged with reporters encoding several kinds of luciferase enzymes, which genera...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/IJBI/2006/57614 |
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| _version_ | 1832567358911152128 |
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| author | Alexander X. Cong Ge Wang |
| author_facet | Alexander X. Cong Ge Wang |
| author_sort | Alexander X. Cong |
| collection | DOAJ |
| description | Bioluminescent imaging has proven to be a valuable tool for
monitoring physiological and pathological activities at cellular
and molecular levels in living small animals. Using biological
techniques, target cells can be tagged with reporters encoding
several kinds of luciferase enzymes, which generate characteristic
photons in a wide spectrum covering the infrared range. Part of
the diffused light can reach the body surface of the small animal,
be separated into several spectral bands using appropriate
filters, and collected by a sensitive CCD camera. Here we present
a bioluminescence tomography (BLT) method for a bioluminescent
source reconstruction from multispectral data measured on the
external surface, and demonstrate the advantages of multispectral
BLT in a numerical study using a heterogeneous mouse chest
phantom. The results show that the multispectral approach
significantly improves the accuracy and stability of the BLT
reconstruction even if the data are highly noisy. |
| format | Article |
| id | doaj-art-21fffe1b9a8243fcbff58cdb474a8476 |
| institution | Kabale University |
| issn | 1687-4188 1687-4196 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomedical Imaging |
| spelling | doaj-art-21fffe1b9a8243fcbff58cdb474a84762025-02-03T01:01:36ZengWileyInternational Journal of Biomedical Imaging1687-41881687-41962006-01-01200610.1155/IJBI/2006/5761457614Multispectral Bioluminescence Tomography: Methodology and SimulationAlexander X. Cong0Ge Wang1Bioluminescence Tomography Laboratory, Department of Radiology, University of Iowa, Iowa City, IA 52242, USABioluminescence Tomography Laboratory, Department of Radiology, University of Iowa, Iowa City, IA 52242, USABioluminescent imaging has proven to be a valuable tool for monitoring physiological and pathological activities at cellular and molecular levels in living small animals. Using biological techniques, target cells can be tagged with reporters encoding several kinds of luciferase enzymes, which generate characteristic photons in a wide spectrum covering the infrared range. Part of the diffused light can reach the body surface of the small animal, be separated into several spectral bands using appropriate filters, and collected by a sensitive CCD camera. Here we present a bioluminescence tomography (BLT) method for a bioluminescent source reconstruction from multispectral data measured on the external surface, and demonstrate the advantages of multispectral BLT in a numerical study using a heterogeneous mouse chest phantom. The results show that the multispectral approach significantly improves the accuracy and stability of the BLT reconstruction even if the data are highly noisy.http://dx.doi.org/10.1155/IJBI/2006/57614 |
| spellingShingle | Alexander X. Cong Ge Wang Multispectral Bioluminescence Tomography: Methodology and Simulation International Journal of Biomedical Imaging |
| title | Multispectral Bioluminescence Tomography: Methodology and Simulation |
| title_full | Multispectral Bioluminescence Tomography: Methodology and Simulation |
| title_fullStr | Multispectral Bioluminescence Tomography: Methodology and Simulation |
| title_full_unstemmed | Multispectral Bioluminescence Tomography: Methodology and Simulation |
| title_short | Multispectral Bioluminescence Tomography: Methodology and Simulation |
| title_sort | multispectral bioluminescence tomography methodology and simulation |
| url | http://dx.doi.org/10.1155/IJBI/2006/57614 |
| work_keys_str_mv | AT alexanderxcong multispectralbioluminescencetomographymethodologyandsimulation AT gewang multispectralbioluminescencetomographymethodologyandsimulation |