Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry

Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry

Summary: Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measur...

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Bibliographic Details
Main Authors: Johannes Krell, Komal Kumar Javarappa, Angie Wenedy, Andrew L. Frelinger, III, Laurent Renia, Clarissa Prazeres da Costa, Martin Schlegel, Percy Knolle, Gerhard Schneider, Oliver Hayden
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Clinical Protocol
Flow Cytometry
Immunology
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000048
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