Generation and characterization of chicken monocyte-derived dendritic cells
IntroductionDendritic cells (DCs) play a crucial role in orchestrating immune responses by bridging innate and adaptive immunity. In vitro generation of DCs from mouse and human tissues such as bone marrow and peripheral blood monocytes, has been widely used to study their immunological functions. I...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1517697/full |
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| author | Elie Ngantcha Tatchou Elie Ngantcha Tatchou Romane Milcamps Romane Milcamps Guillaume Oldenhove Bénédicte Lambrecht Fiona Ingrao |
| author_facet | Elie Ngantcha Tatchou Elie Ngantcha Tatchou Romane Milcamps Romane Milcamps Guillaume Oldenhove Bénédicte Lambrecht Fiona Ingrao |
| author_sort | Elie Ngantcha Tatchou |
| collection | DOAJ |
| description | IntroductionDendritic cells (DCs) play a crucial role in orchestrating immune responses by bridging innate and adaptive immunity. In vitro generation of DCs from mouse and human tissues such as bone marrow and peripheral blood monocytes, has been widely used to study their immunological functions. In chicken, DCs have mainly been derived from bone marrow cell cultures, with limited characterization from blood monocytes.MethodsThe present study takes advantage of newly available chicken immunological tools to further characterize chicken monocyte-derived dendritic cells (MoDCs), focusing on their phenotype, and functions, including antigen capture and T-cell stimulation, and response to live Newcastle disease virus (NDV) stimulation.ResultsAdherent chicken PBMCs were cultured with recombinant chicken granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), for 5 days, leading to the upregulation of putative CD11c and MHCII, markers of DC differentiation. Subsequent stimulation with lipopolysaccharide (LPS) or 24 h triggered phenotypic maturation of MoDCs, characterized by the increased surface expression of MHCII and co-stimulatory molecules CD80 and CD40, and elevated IL-12p40 secretion. This maturation reduced endocytic capacity but enhanced the allogenic stimulatory activity of the chicken MoDCs. Upon NDV stimulation for 6 h, MoDCs upregulated antiviral pathways, including retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), melanoma differentiation-associated protein 5 (MDA5) and laboratory of genetics and physiology 2 (LGP2), alongside increased production of type I interferons (IFNs), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), IL-1β, and IL-6. However, these responses were downregulated after 24 hours.ConclusionThese findings provide a comprehensive characterization of chicken MoDCs and suggest their potential as a model for studying host-pathogen interactions. |
| format | Article |
| id | doaj-art-21cf1a9b9a614472b25a6d6120996ad3 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-21cf1a9b9a614472b25a6d6120996ad32025-02-04T06:31:42ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-02-011610.3389/fimmu.2025.15176971517697Generation and characterization of chicken monocyte-derived dendritic cellsElie Ngantcha Tatchou0Elie Ngantcha Tatchou1Romane Milcamps2Romane Milcamps3Guillaume Oldenhove4Bénédicte Lambrecht5Fiona Ingrao6Service of Avian Virology and Immunology, Sciensano, Brussels, BelgiumLaboratory of Immunobiology, Université Libre de Bruxelles, Gosselies, BelgiumService of Avian Virology and Immunology, Sciensano, Brussels, BelgiumMolecular Virology Unit, de Duve Institute, Université Catholique de Louvain, Brussels, BelgiumLaboratory of Immunobiology, Université Libre de Bruxelles, Gosselies, BelgiumService of Avian Virology and Immunology, Sciensano, Brussels, BelgiumService of Avian Virology and Immunology, Sciensano, Brussels, BelgiumIntroductionDendritic cells (DCs) play a crucial role in orchestrating immune responses by bridging innate and adaptive immunity. In vitro generation of DCs from mouse and human tissues such as bone marrow and peripheral blood monocytes, has been widely used to study their immunological functions. In chicken, DCs have mainly been derived from bone marrow cell cultures, with limited characterization from blood monocytes.MethodsThe present study takes advantage of newly available chicken immunological tools to further characterize chicken monocyte-derived dendritic cells (MoDCs), focusing on their phenotype, and functions, including antigen capture and T-cell stimulation, and response to live Newcastle disease virus (NDV) stimulation.ResultsAdherent chicken PBMCs were cultured with recombinant chicken granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), for 5 days, leading to the upregulation of putative CD11c and MHCII, markers of DC differentiation. Subsequent stimulation with lipopolysaccharide (LPS) or 24 h triggered phenotypic maturation of MoDCs, characterized by the increased surface expression of MHCII and co-stimulatory molecules CD80 and CD40, and elevated IL-12p40 secretion. This maturation reduced endocytic capacity but enhanced the allogenic stimulatory activity of the chicken MoDCs. Upon NDV stimulation for 6 h, MoDCs upregulated antiviral pathways, including retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), melanoma differentiation-associated protein 5 (MDA5) and laboratory of genetics and physiology 2 (LGP2), alongside increased production of type I interferons (IFNs), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), IL-1β, and IL-6. However, these responses were downregulated after 24 hours.ConclusionThese findings provide a comprehensive characterization of chicken MoDCs and suggest their potential as a model for studying host-pathogen interactions.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1517697/fullmonocyte-derived dendritic cellsmaturationin vitrolipopolysaccharideNewcastle disease virus |
| spellingShingle | Elie Ngantcha Tatchou Elie Ngantcha Tatchou Romane Milcamps Romane Milcamps Guillaume Oldenhove Bénédicte Lambrecht Fiona Ingrao Generation and characterization of chicken monocyte-derived dendritic cells Frontiers in Immunology monocyte-derived dendritic cells maturation in vitro lipopolysaccharide Newcastle disease virus |
| title | Generation and characterization of chicken monocyte-derived dendritic cells |
| title_full | Generation and characterization of chicken monocyte-derived dendritic cells |
| title_fullStr | Generation and characterization of chicken monocyte-derived dendritic cells |
| title_full_unstemmed | Generation and characterization of chicken monocyte-derived dendritic cells |
| title_short | Generation and characterization of chicken monocyte-derived dendritic cells |
| title_sort | generation and characterization of chicken monocyte derived dendritic cells |
| topic | monocyte-derived dendritic cells maturation in vitro lipopolysaccharide Newcastle disease virus |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1517697/full |
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