Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens
Proteins in egg whites play vital roles in embryonic development. Simultaneously, protein modification is affected by the surrounding environment, which ultimately affects the structure and function of proteins. Here, we measured the phenotypes of eggs at different time points during storage and inc...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011040 |
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| author | Daqing Dai Xiqiong Wang Kexin Wu Fangren Lan Jiaming Jin Wenxin Zhang Chaoliang Wen Junying Li Ning Yang Congjiao Sun |
| author_facet | Daqing Dai Xiqiong Wang Kexin Wu Fangren Lan Jiaming Jin Wenxin Zhang Chaoliang Wen Junying Li Ning Yang Congjiao Sun |
| author_sort | Daqing Dai |
| collection | DOAJ |
| description | Proteins in egg whites play vital roles in embryonic development. Simultaneously, protein modification is affected by the surrounding environment, which ultimately affects the structure and function of proteins. Here, we measured the phenotypes of eggs at different time points during storage and incubation and used 4D label-free quantitative proteomics technology and liquid chromatography/tandem mass spectrometry (LC-MS/MS)-technique to identify the differential proteins and N-glycosylation sites in egg whites during storage and incubation. We found that the differential N-glycoproteins in the early stage of storage were mainly related to protein structure changes, antibacterial activity, and cell proliferation, and that there were more protease inhibitors in egg whites, which decreased in the later stage of storage. Finally, eleven possible protein markers and N-glycosylation sites were identified to significantly change during storage and may exert an effect on hatchability, including the proteins involved in antibacterial activity (OVOA-N855, CLU-N154, ogchi-N82, PIGR-N290, WFDC2-N120), protein structure (LOC776816), and cell proliferation (ASAH1-N173). This study provides substantial insights into the physical and molecular compositional changes in egg whites under different storage times and revealed their potential effect on chick embryo development. |
| format | Article |
| id | doaj-art-21a58082cc864f48a71166f51d219584 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-21a58082cc864f48a71166f51d2195842025-01-22T05:40:21ZengElsevierPoultry Science0032-57912025-01-011041104526Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickensDaqing Dai0Xiqiong Wang1Kexin Wu2Fangren Lan3Jiaming Jin4Wenxin Zhang5Chaoliang Wen6Junying Li7Ning Yang8Congjiao Sun9State Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaCorresponding author.; State Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding, China Agricultural University, Beijing 100193, ChinaProteins in egg whites play vital roles in embryonic development. Simultaneously, protein modification is affected by the surrounding environment, which ultimately affects the structure and function of proteins. Here, we measured the phenotypes of eggs at different time points during storage and incubation and used 4D label-free quantitative proteomics technology and liquid chromatography/tandem mass spectrometry (LC-MS/MS)-technique to identify the differential proteins and N-glycosylation sites in egg whites during storage and incubation. We found that the differential N-glycoproteins in the early stage of storage were mainly related to protein structure changes, antibacterial activity, and cell proliferation, and that there were more protease inhibitors in egg whites, which decreased in the later stage of storage. Finally, eleven possible protein markers and N-glycosylation sites were identified to significantly change during storage and may exert an effect on hatchability, including the proteins involved in antibacterial activity (OVOA-N855, CLU-N154, ogchi-N82, PIGR-N290, WFDC2-N120), protein structure (LOC776816), and cell proliferation (ASAH1-N173). This study provides substantial insights into the physical and molecular compositional changes in egg whites under different storage times and revealed their potential effect on chick embryo development.http://www.sciencedirect.com/science/article/pii/S0032579124011040Fertile eggChicken egg whiteStorageProteomicsN-glycosylation |
| spellingShingle | Daqing Dai Xiqiong Wang Kexin Wu Fangren Lan Jiaming Jin Wenxin Zhang Chaoliang Wen Junying Li Ning Yang Congjiao Sun Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens Poultry Science Fertile egg Chicken egg white Storage Proteomics N-glycosylation |
| title | Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens |
| title_full | Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens |
| title_fullStr | Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens |
| title_full_unstemmed | Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens |
| title_short | Proteomic and N-glycosylation analysis of fertile egg white during storage and incubation in chickens |
| title_sort | proteomic and n glycosylation analysis of fertile egg white during storage and incubation in chickens |
| topic | Fertile egg Chicken egg white Storage Proteomics N-glycosylation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011040 |
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