Depression and Temporal Lobe Epilepsy: Expression Pattern of Calbindin Immunoreactivity in Hippocampal Dentate Gyrus of Patients Who Underwent Epilepsy Surgery with and without Comorbid Depression
Purpose. Changes in calbindin (CB) expression have been reported in patients with temporal lobe epilepsy (TLE) with controversial implications on hippocampal functions. The aim of this study was to determine the CB immunoreactivity in hippocampal dentate gyrus of patients who underwent epilepsy surg...
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Wiley
2019-01-01
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| Series: | Behavioural Neurology |
| Online Access: | http://dx.doi.org/10.1155/2019/7396793 |
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| author | Luciana D’Alessio Hector Konopka Patricia Solís Laura Scévola Mónica Fernandez Lima Claudio Nuñez Eduardo Seoane Silvia Oddo Silvia Kochen |
| author_facet | Luciana D’Alessio Hector Konopka Patricia Solís Laura Scévola Mónica Fernandez Lima Claudio Nuñez Eduardo Seoane Silvia Oddo Silvia Kochen |
| author_sort | Luciana D’Alessio |
| collection | DOAJ |
| description | Purpose. Changes in calbindin (CB) expression have been reported in patients with temporal lobe epilepsy (TLE) with controversial implications on hippocampal functions. The aim of this study was to determine the CB immunoreactivity in hippocampal dentate gyrus of patients who underwent epilepsy surgery for drug-resistant TLE with and without comorbid depression and/or memory deficits. Methods. Selected hippocampal samples from patients with TLE who underwent epilepsy surgery were included. Clinical and complementary assessment: EEG, video-EEG, MRI, psychiatric assessment (structured clinical interview, DSM-IV), and memory assessment (Rey auditory verbal learning test, RAVLT; Rey-Osterrieth complex figure test, RCFT), were determined before surgery. Hippocampal sections were processed using immunoperoxidase with the anti-calbindin antibody. The semiquantitative analysis of CB immunoreactivity was determined in dentate gyrus by computerized image analysis (ImageJ). Results. Hippocampal sections of patients with TLE and HS (n=24) and postmortem controls (n=5) were included. A significant reduction of CB+ cells was found in patients with TLE (p<0.05, Student’s t-test). Among TLE cases (n=24), depression (n=12) and memory deficit (n=17) were determined. Depression was associated with a higher % of cells with the CB dendritic expression (CB-sprouted cells) (F1,20=11.81, p=0.003, hp2=0.37), a higher CB+ area (μm2) (F1,20=5.33, p=0.032, hp2=0.21), and a higher optical density (F1,20=15.09, p=0.001, hp2=0.43) (two-way ANOVA). The GAF scale (general assessment of functioning) of DSM-IV inversely correlated with the % of CB-sprouted cells (r=−0.52, p=0.008) and with the CB+ area (r=−0.46, p=0.022). Conclusions. In this exploratory study, comorbid depression was associated with a differential pattern of CB cell loss in dentate gyrus combined with a higher CB sprouting. These changes may indicate granular cell dysmaturation associated to the epileptic hyperexcitability phenomena. Further investigations should be carried out to confirm these preliminary findings. |
| format | Article |
| id | doaj-art-218bc2ac06f940319401a94813886014 |
| institution | Kabale University |
| issn | 0953-4180 1875-8584 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Behavioural Neurology |
| spelling | doaj-art-218bc2ac06f940319401a948138860142025-02-03T01:00:31ZengWileyBehavioural Neurology0953-41801875-85842019-01-01201910.1155/2019/73967937396793Depression and Temporal Lobe Epilepsy: Expression Pattern of Calbindin Immunoreactivity in Hippocampal Dentate Gyrus of Patients Who Underwent Epilepsy Surgery with and without Comorbid DepressionLuciana D’Alessio0Hector Konopka1Patricia Solís2Laura Scévola3Mónica Fernandez Lima4Claudio Nuñez5Eduardo Seoane6Silvia Oddo7Silvia Kochen8ENyS-IBCN, Neuroscience and Systems Institute and Cell Biology and Neuroscience Institute, CONICET, National Council for Scientific and Technical Research of Argentina, Universidad de Buenos Aires, Buenos Aires, ArgentinaENyS-IBCN, Neuroscience and Systems Institute and Cell Biology and Neuroscience Institute, CONICET, National Council for Scientific and Technical Research of Argentina, Universidad de Buenos Aires, Buenos Aires, ArgentinaEpilepsy Center of Ramos Mejía and El Cruce Hospitals, Buenos Aires, ArgentinaEpilepsy Center of Ramos Mejía and El Cruce Hospitals, Buenos Aires, ArgentinaENyS-IBCN, Neuroscience and Systems Institute and Cell Biology and Neuroscience Institute, CONICET, National Council for Scientific and Technical Research of Argentina, Universidad de Buenos Aires, Buenos Aires, ArgentinaEpilepsy Center of Ramos Mejía and El Cruce Hospitals, Buenos Aires, ArgentinaEpilepsy Center of Ramos Mejía and El Cruce Hospitals, Buenos Aires, ArgentinaENyS-IBCN, Neuroscience and Systems Institute and Cell Biology and Neuroscience Institute, CONICET, National Council for Scientific and Technical Research of Argentina, Universidad de Buenos Aires, Buenos Aires, ArgentinaENyS-IBCN, Neuroscience and Systems Institute and Cell Biology and Neuroscience Institute, CONICET, National Council for Scientific and Technical Research of Argentina, Universidad de Buenos Aires, Buenos Aires, ArgentinaPurpose. Changes in calbindin (CB) expression have been reported in patients with temporal lobe epilepsy (TLE) with controversial implications on hippocampal functions. The aim of this study was to determine the CB immunoreactivity in hippocampal dentate gyrus of patients who underwent epilepsy surgery for drug-resistant TLE with and without comorbid depression and/or memory deficits. Methods. Selected hippocampal samples from patients with TLE who underwent epilepsy surgery were included. Clinical and complementary assessment: EEG, video-EEG, MRI, psychiatric assessment (structured clinical interview, DSM-IV), and memory assessment (Rey auditory verbal learning test, RAVLT; Rey-Osterrieth complex figure test, RCFT), were determined before surgery. Hippocampal sections were processed using immunoperoxidase with the anti-calbindin antibody. The semiquantitative analysis of CB immunoreactivity was determined in dentate gyrus by computerized image analysis (ImageJ). Results. Hippocampal sections of patients with TLE and HS (n=24) and postmortem controls (n=5) were included. A significant reduction of CB+ cells was found in patients with TLE (p<0.05, Student’s t-test). Among TLE cases (n=24), depression (n=12) and memory deficit (n=17) were determined. Depression was associated with a higher % of cells with the CB dendritic expression (CB-sprouted cells) (F1,20=11.81, p=0.003, hp2=0.37), a higher CB+ area (μm2) (F1,20=5.33, p=0.032, hp2=0.21), and a higher optical density (F1,20=15.09, p=0.001, hp2=0.43) (two-way ANOVA). The GAF scale (general assessment of functioning) of DSM-IV inversely correlated with the % of CB-sprouted cells (r=−0.52, p=0.008) and with the CB+ area (r=−0.46, p=0.022). Conclusions. In this exploratory study, comorbid depression was associated with a differential pattern of CB cell loss in dentate gyrus combined with a higher CB sprouting. These changes may indicate granular cell dysmaturation associated to the epileptic hyperexcitability phenomena. Further investigations should be carried out to confirm these preliminary findings.http://dx.doi.org/10.1155/2019/7396793 |
| spellingShingle | Luciana D’Alessio Hector Konopka Patricia Solís Laura Scévola Mónica Fernandez Lima Claudio Nuñez Eduardo Seoane Silvia Oddo Silvia Kochen Depression and Temporal Lobe Epilepsy: Expression Pattern of Calbindin Immunoreactivity in Hippocampal Dentate Gyrus of Patients Who Underwent Epilepsy Surgery with and without Comorbid Depression Behavioural Neurology |
| title | Depression and Temporal Lobe Epilepsy: Expression Pattern of Calbindin Immunoreactivity in Hippocampal Dentate Gyrus of Patients Who Underwent Epilepsy Surgery with and without Comorbid Depression |
| title_full | Depression and Temporal Lobe Epilepsy: Expression Pattern of Calbindin Immunoreactivity in Hippocampal Dentate Gyrus of Patients Who Underwent Epilepsy Surgery with and without Comorbid Depression |
| title_fullStr | Depression and Temporal Lobe Epilepsy: Expression Pattern of Calbindin Immunoreactivity in Hippocampal Dentate Gyrus of Patients Who Underwent Epilepsy Surgery with and without Comorbid Depression |
| title_full_unstemmed | Depression and Temporal Lobe Epilepsy: Expression Pattern of Calbindin Immunoreactivity in Hippocampal Dentate Gyrus of Patients Who Underwent Epilepsy Surgery with and without Comorbid Depression |
| title_short | Depression and Temporal Lobe Epilepsy: Expression Pattern of Calbindin Immunoreactivity in Hippocampal Dentate Gyrus of Patients Who Underwent Epilepsy Surgery with and without Comorbid Depression |
| title_sort | depression and temporal lobe epilepsy expression pattern of calbindin immunoreactivity in hippocampal dentate gyrus of patients who underwent epilepsy surgery with and without comorbid depression |
| url | http://dx.doi.org/10.1155/2019/7396793 |
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