Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies
IntroductionThis study investigated the antioxidant, antimicrobial, and insecticidal properties of Chamaemelum nobile (L.) essential oil (CN-EO), harvested in Taounate, Morocco. The molecular composition and chemical profile of CN-EO were also characterized.MethodsThe CN-EO was extracted using a Cle...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2025.1539872/full |
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| author | El-Mehdi El-Assri El-Mehdi El-Assri Youssef El-Assri Rajae El Brahimi Mohamed El fadili Asmae Baghouz Hatem A. Abuelizz Sara Er-Rahmani Amal Lahkimi Abdelhak Bouia |
| author_facet | El-Mehdi El-Assri El-Mehdi El-Assri Youssef El-Assri Rajae El Brahimi Mohamed El fadili Asmae Baghouz Hatem A. Abuelizz Sara Er-Rahmani Amal Lahkimi Abdelhak Bouia |
| author_sort | El-Mehdi El-Assri |
| collection | DOAJ |
| description | IntroductionThis study investigated the antioxidant, antimicrobial, and insecticidal properties of Chamaemelum nobile (L.) essential oil (CN-EO), harvested in Taounate, Morocco. The molecular composition and chemical profile of CN-EO were also characterized.MethodsThe CN-EO was extracted using a Clevenger apparatus. Its chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS). Antioxidant activity was evaluated using the DPPH assay, while antimicrobial properties were assessed via the disk diffusion method to measure inhibition zones against various bacterial and fungal strains. Insecticidal activity was tested through bioassays to determine insect mortality and repellency rates. Phylogenetic analysis of DNA sequences was conducted to confirm the species identity.ResultsGC-MS analysis identified 24 compounds in CN-EO, with β-Oplopenone (18.66%), Spathulenol (14.90%), and Himachalene (12.47%) as major constituents. CN-EO exhibited strong antioxidant activity (IC50 = 135.8 ± 1.03 μg/mL). Antimicrobial assays revealed inhibition zones of up to 20.67 ± 0.58 mm (Staphylococcus aureus) and antifungal inhibition of 40.42% ± 2.82% against Aspergillus flavus. Insecticidal tests showed total insect mortality at 166 µL/L within 48 h and a 60% repellent effect. Phylogenetic analysis of the DNA sequence revealed a 99.22% similarity with Chamaemelum nobile (L.).ConclusionThese results demonstrate the significant potential of Moroccan CN-EO in phytomedicine. It exhibits a wide range of biological activities and shows great promise as a natural antioxidant, antimicrobial agent, antifungal, and insecticide. |
| format | Article |
| id | doaj-art-21576bf8a282427caa66aa8ed20ee750 |
| institution | Kabale University |
| issn | 2296-2646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Chemistry |
| spelling | doaj-art-21576bf8a282427caa66aa8ed20ee7502025-02-04T06:31:52ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462025-02-011310.3389/fchem.2025.15398721539872Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studiesEl-Mehdi El-Assri0El-Mehdi El-Assri1Youssef El-Assri2Rajae El Brahimi3Mohamed El fadili4Asmae Baghouz5Hatem A. Abuelizz6Sara Er-Rahmani7Amal Lahkimi8Abdelhak Bouia9Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoLaboratory of Engineering, Electrochemistry, Modeling and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoLaboratory of Engineering, Electrochemistry, Modeling and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoLaboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoLIMAS Laboratory, Department of chemistry, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoLaboratory of Biotechnology, Conservation and Valorisation of Naturals Resources, Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Chemistry, Nis Interdepartmental Centre, University of Turin, Turin, ItalyLaboratory of Engineering, Electrochemistry, Modeling and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoLaboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoIntroductionThis study investigated the antioxidant, antimicrobial, and insecticidal properties of Chamaemelum nobile (L.) essential oil (CN-EO), harvested in Taounate, Morocco. The molecular composition and chemical profile of CN-EO were also characterized.MethodsThe CN-EO was extracted using a Clevenger apparatus. Its chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS). Antioxidant activity was evaluated using the DPPH assay, while antimicrobial properties were assessed via the disk diffusion method to measure inhibition zones against various bacterial and fungal strains. Insecticidal activity was tested through bioassays to determine insect mortality and repellency rates. Phylogenetic analysis of DNA sequences was conducted to confirm the species identity.ResultsGC-MS analysis identified 24 compounds in CN-EO, with β-Oplopenone (18.66%), Spathulenol (14.90%), and Himachalene (12.47%) as major constituents. CN-EO exhibited strong antioxidant activity (IC50 = 135.8 ± 1.03 μg/mL). Antimicrobial assays revealed inhibition zones of up to 20.67 ± 0.58 mm (Staphylococcus aureus) and antifungal inhibition of 40.42% ± 2.82% against Aspergillus flavus. Insecticidal tests showed total insect mortality at 166 µL/L within 48 h and a 60% repellent effect. Phylogenetic analysis of the DNA sequence revealed a 99.22% similarity with Chamaemelum nobile (L.).ConclusionThese results demonstrate the significant potential of Moroccan CN-EO in phytomedicine. It exhibits a wide range of biological activities and shows great promise as a natural antioxidant, antimicrobial agent, antifungal, and insecticide.https://www.frontiersin.org/articles/10.3389/fchem.2025.1539872/fullantimicrobial activityantioxidant activityChamaemelum nobile (L.)essential oilGC-MSin silico studies |
| spellingShingle | El-Mehdi El-Assri El-Mehdi El-Assri Youssef El-Assri Rajae El Brahimi Mohamed El fadili Asmae Baghouz Hatem A. Abuelizz Sara Er-Rahmani Amal Lahkimi Abdelhak Bouia Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies Frontiers in Chemistry antimicrobial activity antioxidant activity Chamaemelum nobile (L.) essential oil GC-MS in silico studies |
| title | Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies |
| title_full | Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies |
| title_fullStr | Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies |
| title_full_unstemmed | Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies |
| title_short | Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies |
| title_sort | molecular characterization chemical profile and biological properties of essential oils from chamaemelum nobile l flowers of morocco in vitro and in silico studies |
| topic | antimicrobial activity antioxidant activity Chamaemelum nobile (L.) essential oil GC-MS in silico studies |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2025.1539872/full |
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