Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry Industry
This work aimed to develop a nanosuspension (NSps) as an acetogenin (ACGs) carrier, using soy lecithin (SL) and hydroxypropyl-β-cyclodextrin (βCD) named NSps-βCDSL-ACGs. It was characterized by various spectroscopic techniques (DLS, FTIR, UV-vis diffuse reflectance). Moreover, the NSps morphology wa...
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MDPI AG
2024-12-01
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| Series: | Microorganisms |
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| author | Brandon A. López-Romero Gabriela Aguilar-Hernández Billy M. Hargis María de Lourdes García-Magaña Ulises M. López-García Rosa I. Ortiz-Basurto Adalberto Zamudio-Ojeda Juan D. Latorre Efigenia Montalvo-González |
| author_facet | Brandon A. López-Romero Gabriela Aguilar-Hernández Billy M. Hargis María de Lourdes García-Magaña Ulises M. López-García Rosa I. Ortiz-Basurto Adalberto Zamudio-Ojeda Juan D. Latorre Efigenia Montalvo-González |
| author_sort | Brandon A. López-Romero |
| collection | DOAJ |
| description | This work aimed to develop a nanosuspension (NSps) as an acetogenin (ACGs) carrier, using soy lecithin (SL) and hydroxypropyl-β-cyclodextrin (βCD) named NSps-βCDSL-ACGs. It was characterized by various spectroscopic techniques (DLS, FTIR, UV-vis diffuse reflectance). Moreover, the NSps morphology was observed by transmission electron microscopy (TEM). Also, the antibacterial activity of NSps-βCDSL-ACGs was evaluated against strains of interest in the poultry sector. NSps-βCDSL-ACGs presented nanometric size (207–239 nm), acceptable polydispersity index (PDI) values (0.13–0.17) and a high Z potential value (−47.17–50.36 mV), demonstrating high stability. The presence of ACGs in NSps-βCDSL-ACGs was confirmed by FTIR analysis. The nanoparticles had a spherical shape and exhibited high inhibition potential against <i>Salmonella</i> Enteritidis (88.18%), <i>Streptococcus gallolyticus</i> (88.01%), <i>Salmonella</i> Typhimurium (86.28%) and <i>Salmonella</i> Infantis (77.02%) strains up to 48 h, and a reduction of up to 3 log CFU/mL was achieved for <i>S.</i> Typhimurium. Therefore, NSps-βCDSL-ACGs is an attractive option for implementing ACGs administration in the poultry sector to reduce the use of antibiotics and minimize bacterial resistance. |
| format | Article |
| id | doaj-art-20f01bc2b57f4f688937799a0d764366 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-20f01bc2b57f4f688937799a0d7643662025-01-24T13:42:18ZengMDPI AGMicroorganisms2076-26072024-12-011311810.3390/microorganisms13010018Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry IndustryBrandon A. López-Romero0Gabriela Aguilar-Hernández1Billy M. Hargis2María de Lourdes García-Magaña3Ulises M. López-García4Rosa I. Ortiz-Basurto5Adalberto Zamudio-Ojeda6Juan D. Latorre7Efigenia Montalvo-González8Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México, Instituto Tecnológico de Tepic, Tepic 63175, MexicoDivisión de Ciencias Agropecuarias e Ingenierías, Centro Universitario de los Altos, Universidad de Guadalajara, Guadalajara 47600, MexicoDepartment of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USALaboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México, Instituto Tecnológico de Tepic, Tepic 63175, MexicoLaboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México, Instituto Tecnológico de Tepic, Tepic 63175, MexicoLaboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México, Instituto Tecnológico de Tepic, Tepic 63175, MexicoDepartamento de Física y Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Guadalajara 44430, MexicoDepartment of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USALaboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México, Instituto Tecnológico de Tepic, Tepic 63175, MexicoThis work aimed to develop a nanosuspension (NSps) as an acetogenin (ACGs) carrier, using soy lecithin (SL) and hydroxypropyl-β-cyclodextrin (βCD) named NSps-βCDSL-ACGs. It was characterized by various spectroscopic techniques (DLS, FTIR, UV-vis diffuse reflectance). Moreover, the NSps morphology was observed by transmission electron microscopy (TEM). Also, the antibacterial activity of NSps-βCDSL-ACGs was evaluated against strains of interest in the poultry sector. NSps-βCDSL-ACGs presented nanometric size (207–239 nm), acceptable polydispersity index (PDI) values (0.13–0.17) and a high Z potential value (−47.17–50.36 mV), demonstrating high stability. The presence of ACGs in NSps-βCDSL-ACGs was confirmed by FTIR analysis. The nanoparticles had a spherical shape and exhibited high inhibition potential against <i>Salmonella</i> Enteritidis (88.18%), <i>Streptococcus gallolyticus</i> (88.01%), <i>Salmonella</i> Typhimurium (86.28%) and <i>Salmonella</i> Infantis (77.02%) strains up to 48 h, and a reduction of up to 3 log CFU/mL was achieved for <i>S.</i> Typhimurium. Therefore, NSps-βCDSL-ACGs is an attractive option for implementing ACGs administration in the poultry sector to reduce the use of antibiotics and minimize bacterial resistance.https://www.mdpi.com/2076-2607/13/1/18acetogeninsantibacterial activitynanosuspensionspoultry sector |
| spellingShingle | Brandon A. López-Romero Gabriela Aguilar-Hernández Billy M. Hargis María de Lourdes García-Magaña Ulises M. López-García Rosa I. Ortiz-Basurto Adalberto Zamudio-Ojeda Juan D. Latorre Efigenia Montalvo-González Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry Industry Microorganisms acetogenins antibacterial activity nanosuspensions poultry sector |
| title | Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry Industry |
| title_full | Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry Industry |
| title_fullStr | Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry Industry |
| title_full_unstemmed | Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry Industry |
| title_short | Characterization of an Acetogenin-Carrying Nanosuspension and Its Effect on Bacteria of Interest in the Poultry Industry |
| title_sort | characterization of an acetogenin carrying nanosuspension and its effect on bacteria of interest in the poultry industry |
| topic | acetogenins antibacterial activity nanosuspensions poultry sector |
| url | https://www.mdpi.com/2076-2607/13/1/18 |
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