High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD
Intestinal alterations in IBD are triggered and maintained by an overexpression of proinflammatory cytokines. Additionally, increased immune activation has been found in the adjacent intestinal areas without displaying any apparent histological alterations, however, the regulatory environment is not...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/580450 |
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| author | Alberto J. León Emma Gómez Jose A. Garrote David Bernardo Asterio Barrera Jose L. Marcos Luis Fernández-Salazar Benito Velayos Alfredo Blanco-Quirós Eduardo Arranz |
| author_facet | Alberto J. León Emma Gómez Jose A. Garrote David Bernardo Asterio Barrera Jose L. Marcos Luis Fernández-Salazar Benito Velayos Alfredo Blanco-Quirós Eduardo Arranz |
| author_sort | Alberto J. León |
| collection | DOAJ |
| description | Intestinal alterations in IBD are triggered and maintained by an overexpression of proinflammatory cytokines. Additionally, increased immune activation has been found in the adjacent intestinal areas without displaying any apparent histological alterations, however, the regulatory environment is not well established. Biopsy specimens from patients with ulcerative colitis (UC) and Crohn's disease (CD), from both affected and unaffected areas, and also from a group of colonic biopsies from healthy controls, were included in our study. Cytokines and markers of mucosal damage were analyzed by real-time PCR, and some of the results confirmed by western-blot and ELISA. Levels of IFNγ, TNFα, IL-6, IL-15, IL-18, and IL-23 were increased (above healthy controls) in both affected and unaffected areas from IBD. IL-1β, IL-6, IL-12, and IL-27 were higher in affected areas compared to unaffected ones in UC but not CD. In general, a correlation was observed between mRNA levels of these cytokines and both iNOS and Granzyme B. SOCS-2 and SOCS-3 were also increased in the affected areas. In conclusion, the unaffected areas from IBD show increased levels of a restricted set of cytokines that may exert immune activating roles in these areas without being able to trigger tissue damage. |
| format | Article |
| id | doaj-art-20e0efe64be34f13a7c31f063ac63788 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-20e0efe64be34f13a7c31f063ac637882025-02-03T06:11:37ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/580450580450High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBDAlberto J. León0Emma Gómez1Jose A. Garrote2David Bernardo3Asterio Barrera4Jose L. Marcos5Luis Fernández-Salazar6Benito Velayos7Alfredo Blanco-Quirós8Eduardo Arranz9Mucosal Immunology Laboratory, Department of Pediatrics & Immunology, IBGM, University of Valladolid, 47005 Valladolid, SpainMucosal Immunology Laboratory, Department of Pediatrics & Immunology, IBGM, University of Valladolid, 47005 Valladolid, SpainMucosal Immunology Laboratory, Department of Pediatrics & Immunology, IBGM, University of Valladolid, 47005 Valladolid, SpainMucosal Immunology Laboratory, Department of Pediatrics & Immunology, IBGM, University of Valladolid, 47005 Valladolid, SpainGeneral Surgery Unit, Hospital Universitario Rio-Hortega, 47012 Valladolid, SpainGeneral Surgery Unit, Hospital Universitario Rio-Hortega, 47012 Valladolid, SpainGastroenterology Unit, Hospital Clínico Universitario, 47011 Valladolid, SpainGastroenterology Unit, Hospital Clínico Universitario, 47011 Valladolid, SpainMucosal Immunology Laboratory, Department of Pediatrics & Immunology, IBGM, University of Valladolid, 47005 Valladolid, SpainMucosal Immunology Laboratory, Department of Pediatrics & Immunology, IBGM, University of Valladolid, 47005 Valladolid, SpainIntestinal alterations in IBD are triggered and maintained by an overexpression of proinflammatory cytokines. Additionally, increased immune activation has been found in the adjacent intestinal areas without displaying any apparent histological alterations, however, the regulatory environment is not well established. Biopsy specimens from patients with ulcerative colitis (UC) and Crohn's disease (CD), from both affected and unaffected areas, and also from a group of colonic biopsies from healthy controls, were included in our study. Cytokines and markers of mucosal damage were analyzed by real-time PCR, and some of the results confirmed by western-blot and ELISA. Levels of IFNγ, TNFα, IL-6, IL-15, IL-18, and IL-23 were increased (above healthy controls) in both affected and unaffected areas from IBD. IL-1β, IL-6, IL-12, and IL-27 were higher in affected areas compared to unaffected ones in UC but not CD. In general, a correlation was observed between mRNA levels of these cytokines and both iNOS and Granzyme B. SOCS-2 and SOCS-3 were also increased in the affected areas. In conclusion, the unaffected areas from IBD show increased levels of a restricted set of cytokines that may exert immune activating roles in these areas without being able to trigger tissue damage.http://dx.doi.org/10.1155/2009/580450 |
| spellingShingle | Alberto J. León Emma Gómez Jose A. Garrote David Bernardo Asterio Barrera Jose L. Marcos Luis Fernández-Salazar Benito Velayos Alfredo Blanco-Quirós Eduardo Arranz High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD Mediators of Inflammation |
| title | High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD |
| title_full | High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD |
| title_fullStr | High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD |
| title_full_unstemmed | High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD |
| title_short | High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD |
| title_sort | high levels of proinflammatory cytokines but not markers of tissue injury in unaffected intestinal areas from patients with ibd |
| url | http://dx.doi.org/10.1155/2009/580450 |
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