Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood
Background. We have shown that approximately 30% of human peripheral blood B-cells express CD25. B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity. The aim of the present study was to character...
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Wiley
2011-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2011/481948 |
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| author | Sylvie Amu Mikael Brisslert |
| author_facet | Sylvie Amu Mikael Brisslert |
| author_sort | Sylvie Amu |
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| description | Background. We have shown that approximately 30% of human peripheral blood B-cells express CD25. B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity. The aim of the present study was to characterize the CD25-expressing subset of B cells in human cord blood. Material and Methods. Mononuclear cell fraction from human cord blood (n=34) and peripheral adult blood (n=22) was sorted into CD20+CD25+ and CD20+CD25- B-cell populations. Phenotype and function of these B-cell populations were compared using flow cytometry, proliferation, cytokine production, and immunoglobulin secretion. Results. CD25-expressing B cells are a limited population of cord blood mononuclear cells representing 5% of the CD20+ B cells. They are characterised by high expression of CD5 in cord blood and CD27 in adult blood. CD25-expressing B cells express a functional IL-2 receptor and high levels of CC-chemokine receptors and spontaneously produce antibodies of IgG and IgM subclass. Conclusions. CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation, possibly ready to migrate and home into the peripheral tissue for further differentiation/action. |
| format | Article |
| id | doaj-art-20dde27af1b643f6be66310959db50b6 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
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| series | Clinical and Developmental Immunology |
| spelling | doaj-art-20dde27af1b643f6be66310959db50b62025-02-03T01:03:38ZengWileyClinical and Developmental Immunology1740-25221740-25302011-01-01201110.1155/2011/481948481948Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord BloodSylvie Amu0Mikael Brisslert1Department of Rheumatology and Inflammation Research, Sahlgrenska Academy at Göteborg University, P.O. Box 480, 405 30 Göteborg, SwedenDepartment of Rheumatology and Inflammation Research, Sahlgrenska Academy at Göteborg University, P.O. Box 480, 405 30 Göteborg, SwedenBackground. We have shown that approximately 30% of human peripheral blood B-cells express CD25. B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity. The aim of the present study was to characterize the CD25-expressing subset of B cells in human cord blood. Material and Methods. Mononuclear cell fraction from human cord blood (n=34) and peripheral adult blood (n=22) was sorted into CD20+CD25+ and CD20+CD25- B-cell populations. Phenotype and function of these B-cell populations were compared using flow cytometry, proliferation, cytokine production, and immunoglobulin secretion. Results. CD25-expressing B cells are a limited population of cord blood mononuclear cells representing 5% of the CD20+ B cells. They are characterised by high expression of CD5 in cord blood and CD27 in adult blood. CD25-expressing B cells express a functional IL-2 receptor and high levels of CC-chemokine receptors and spontaneously produce antibodies of IgG and IgM subclass. Conclusions. CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation, possibly ready to migrate and home into the peripheral tissue for further differentiation/action.http://dx.doi.org/10.1155/2011/481948 |
| spellingShingle | Sylvie Amu Mikael Brisslert Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood Clinical and Developmental Immunology |
| title | Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood |
| title_full | Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood |
| title_fullStr | Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood |
| title_full_unstemmed | Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood |
| title_short | Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood |
| title_sort | phenotype and function of cd25 expressing b lymphocytes isolated from human umbilical cord blood |
| url | http://dx.doi.org/10.1155/2011/481948 |
| work_keys_str_mv | AT sylvieamu phenotypeandfunctionofcd25expressingblymphocytesisolatedfromhumanumbilicalcordblood AT mikaelbrisslert phenotypeandfunctionofcd25expressingblymphocytesisolatedfromhumanumbilicalcordblood |