Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia
ABSTRACT Background Birds are known to harbour many pathogens, including circovirus, herpesviruses, adenoviruses and Chlamydia psittaci. Some of these pose zoonotic risks, while others, such as beak and feather disease virus (BFDV), have a significant impact on the conservation of endangered bird sp...
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70083 |
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| author | Babu Kanti Nath Suman Das Gupta Saranika talukder Nasrin Sultana Tonu Shane R. Raidal Jade K. Forwood Subir Sarker |
| author_facet | Babu Kanti Nath Suman Das Gupta Saranika talukder Nasrin Sultana Tonu Shane R. Raidal Jade K. Forwood Subir Sarker |
| author_sort | Babu Kanti Nath |
| collection | DOAJ |
| description | ABSTRACT Background Birds are known to harbour many pathogens, including circovirus, herpesviruses, adenoviruses and Chlamydia psittaci. Some of these pose zoonotic risks, while others, such as beak and feather disease virus (BFDV), have a significant impact on the conservation of endangered bird species. Objectives This study was aimed to determine the faecal virome of a group of apparently healthy Monk parakeet using high‐throughput sequencing. Methods Fresh faecal samples were collected from four Monk parakeets at a pet shop in Melbourne, Australia. Virus enrichment and nucleic acid extraction were performed on the faecal samples, followed by high‐throughput sequencing at the Australian Genome Research Facility (AGRF). Results Utilising an established pipeline for high‐throughput sequencing data analysis, this study revealed the presence of three viruses of the families Circoviridae, Parvoviridae and Adenoviridae. Subsequent sequence comparison and phylogenetic analyses further confirmed that the detected viruses belong to the genera Chaphamaparvovirus (unassigned species), Circovirus (species Circovirus parrot) and Siadenovirus (species Siadenovirus viridis). Conclusion Despite non‐pathogenicity, the existence of multiple viruses within a bird species underscores the risk of these viruses spreading into the pet trade. Detection and a better understanding of avian viruses are crucial for the establishment of appropriate management and biosecurity measures in the domestic and international bird trade, which ultimately supports the conservation of vulnerable bird species. |
| format | Article |
| id | doaj-art-20c51ec6cc8743ac85e7bfcf7cd73533 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-20c51ec6cc8743ac85e7bfcf7cd735332025-01-20T17:16:44ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70083Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in AustraliaBabu Kanti Nath0Suman Das Gupta1Saranika talukder2Nasrin Sultana Tonu3Shane R. Raidal4Jade K. Forwood5Subir Sarker6School of Agricultural Environmental and Veterinary Sciences Faculty of Science and Health Charles Sturt University Wagga Wagga New South Wales AustraliaSchool of Agricultural Environmental and Veterinary Sciences Faculty of Science and Health Charles Sturt University Wagga Wagga New South Wales AustraliaCollege of Public Health Medical and Veterinary Sciences James Cook University Townsville Queensland AustraliaUpazila Livestock Office and Veterinary Hospital Cumilla BangladeshSchool of Agricultural Environmental and Veterinary Sciences Faculty of Science and Health Charles Sturt University Wagga Wagga New South Wales AustraliaBiosecurity Research Program and Training Centre Gulbali Institute Charles Sturt University Wagga Wagga New South Wales AustraliaBiomedical Sciences & Molecular Biology College of Public Health Medical and Veterinary Sciences James Cook University Townsville Queensland AustraliaABSTRACT Background Birds are known to harbour many pathogens, including circovirus, herpesviruses, adenoviruses and Chlamydia psittaci. Some of these pose zoonotic risks, while others, such as beak and feather disease virus (BFDV), have a significant impact on the conservation of endangered bird species. Objectives This study was aimed to determine the faecal virome of a group of apparently healthy Monk parakeet using high‐throughput sequencing. Methods Fresh faecal samples were collected from four Monk parakeets at a pet shop in Melbourne, Australia. Virus enrichment and nucleic acid extraction were performed on the faecal samples, followed by high‐throughput sequencing at the Australian Genome Research Facility (AGRF). Results Utilising an established pipeline for high‐throughput sequencing data analysis, this study revealed the presence of three viruses of the families Circoviridae, Parvoviridae and Adenoviridae. Subsequent sequence comparison and phylogenetic analyses further confirmed that the detected viruses belong to the genera Chaphamaparvovirus (unassigned species), Circovirus (species Circovirus parrot) and Siadenovirus (species Siadenovirus viridis). Conclusion Despite non‐pathogenicity, the existence of multiple viruses within a bird species underscores the risk of these viruses spreading into the pet trade. Detection and a better understanding of avian viruses are crucial for the establishment of appropriate management and biosecurity measures in the domestic and international bird trade, which ultimately supports the conservation of vulnerable bird species.https://doi.org/10.1002/vms3.70083adenovirusbeak and feather disease viruschaphamaparvovirusmetagenomicsnext‐generation sequencingparrot |
| spellingShingle | Babu Kanti Nath Suman Das Gupta Saranika talukder Nasrin Sultana Tonu Shane R. Raidal Jade K. Forwood Subir Sarker Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia Veterinary Medicine and Science adenovirus beak and feather disease virus chaphamaparvovirus metagenomics next‐generation sequencing parrot |
| title | Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia |
| title_full | Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia |
| title_fullStr | Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia |
| title_full_unstemmed | Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia |
| title_short | Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia |
| title_sort | metagenomic detection of multiple viruses in monk parakeet myiopsitta monachus in australia |
| topic | adenovirus beak and feather disease virus chaphamaparvovirus metagenomics next‐generation sequencing parrot |
| url | https://doi.org/10.1002/vms3.70083 |
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