Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway
This study evaluated the anti-inflammation effect of the three main short-chain fatty acids (SCFAs) on Acinetobacter baumannii-induced THP-1 cells. The three main SCFAs could inhibit A. baumannii-stimulated THP-1 cell NF-κB pathway activity and the expressions of NLRP3 inflamma-some and GSDMD, and i...
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| Format: | Article |
| Language: | English |
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Sciendo
2022-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2022-0036 |
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| author | Shu Chen Yan-Yan Zhang Hai Zhang Long-Kun Ding Yue Xi Man Yan Chang Sun Liang Wu Hao Hu |
| author_facet | Shu Chen Yan-Yan Zhang Hai Zhang Long-Kun Ding Yue Xi Man Yan Chang Sun Liang Wu Hao Hu |
| author_sort | Shu Chen |
| collection | DOAJ |
| description | This study evaluated the anti-inflammation effect of the three main short-chain fatty acids (SCFAs) on Acinetobacter baumannii-induced THP-1 cells. The three main SCFAs could inhibit A. baumannii-stimulated THP-1 cell NF-κB pathway activity and the expressions of NLRP3 inflamma-some and GSDMD, and increase autophagy. The three main SCFAs, especially the sodium butyrate (NaB), had the effect of down-regulation of ROS and TLR-2 expression in THP-1 cells. NaB and sodium propionate (NaPc), but not sodium acetate (NaAc), dramatically suppressed IL-1β and IFN-γ expression. The results indicated that NaB and NaPc could significantly inhibit the inflammation of THP-1 cells induced by A. baumannii, and the inhibitory effect was in the order of NaB > NaPc > NaAC. NaB and NaPc may inhibit inflammation through TLR-2/NF-κB/ROS/NLRP3 signaling pathway. |
| format | Article |
| id | doaj-art-20c2ad0222fc4e16b58023381ce7d347 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-20c2ad0222fc4e16b58023381ce7d3472025-02-02T23:44:33ZengSciendoActa Pharmaceutica1846-95582022-12-0172461562810.2478/acph-2022-0036Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathwayShu Chen0Yan-Yan Zhang1Hai Zhang2Long-Kun Ding3Yue Xi4Man Yan5Chang Sun6Liang Wu7Hao Hu8General Surgery Department, The First Affiliated Hospital of Soochow University Suzhou, Jiangsu, 215006, ChinaTesting Center, Yangzhou University Wenhui Road No. 48, Yangzhou, Jiangsu 225009, ChinaGeneral Surgery Department, Affiliated Hospital of Jiangsu University, Jiefang Road No. 438, Zhenjiang, Jiangsu 212000, ChinaSchool of Medicine, Jiangsu University Xuefu Road No. 301, Zhenjiang, Jiangsu 212013, ChinaSchool of Medicine, Jiangsu University Xuefu Road No. 301, Zhenjiang, Jiangsu 212013, ChinaSchool of Medicine, Jiangsu University Xuefu Road No. 301, Zhenjiang, Jiangsu 212013, ChinaSchool of Medicine, Jiangsu University Xuefu Road No. 301, Zhenjiang, Jiangsu 212013, ChinaSchool of Medicine, Jiangsu University Xuefu Road No. 301, Zhenjiang, Jiangsu 212013, ChinaGeneral Surgery Department, The First Affiliated Hospital of Soochow University Suzhou, Jiangsu, 215006, ChinaThis study evaluated the anti-inflammation effect of the three main short-chain fatty acids (SCFAs) on Acinetobacter baumannii-induced THP-1 cells. The three main SCFAs could inhibit A. baumannii-stimulated THP-1 cell NF-κB pathway activity and the expressions of NLRP3 inflamma-some and GSDMD, and increase autophagy. The three main SCFAs, especially the sodium butyrate (NaB), had the effect of down-regulation of ROS and TLR-2 expression in THP-1 cells. NaB and sodium propionate (NaPc), but not sodium acetate (NaAc), dramatically suppressed IL-1β and IFN-γ expression. The results indicated that NaB and NaPc could significantly inhibit the inflammation of THP-1 cells induced by A. baumannii, and the inhibitory effect was in the order of NaB > NaPc > NaAC. NaB and NaPc may inhibit inflammation through TLR-2/NF-κB/ROS/NLRP3 signaling pathway.https://doi.org/10.2478/acph-2022-0036short-chain fatty acids (scfas)autophagynlrp3nuclear factor-κb (nf-κb)inflammation |
| spellingShingle | Shu Chen Yan-Yan Zhang Hai Zhang Long-Kun Ding Yue Xi Man Yan Chang Sun Liang Wu Hao Hu Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway Acta Pharmaceutica short-chain fatty acids (scfas) autophagy nlrp3 nuclear factor-κb (nf-κb) inflammation |
| title | Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway |
| title_full | Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway |
| title_fullStr | Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway |
| title_full_unstemmed | Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway |
| title_short | Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway |
| title_sort | anti inflammatory effects of nab and napc in acinetobacter baumannii stimulated thp 1 cells via tlr 2 nf κb ros nlrp3 pathway |
| topic | short-chain fatty acids (scfas) autophagy nlrp3 nuclear factor-κb (nf-κb) inflammation |
| url | https://doi.org/10.2478/acph-2022-0036 |
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