Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?
Lipid rafts are dynamic microdomains in the membrane, rich in cholesterol and sphingolipids, that are critical for biological processes like cell signalling, membrane trafficking, and protein organization. Their essential role is claimed in both physiological and pathological conditions, including c...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Membranes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0375/15/1/6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587976659435520 |
|---|---|
| author | Sara Anselmo Elisa Bonaccorso Chiara Gangemi Giuseppe Sancataldo Valeria Conti Nibali Giovanna D’Angelo |
| author_facet | Sara Anselmo Elisa Bonaccorso Chiara Gangemi Giuseppe Sancataldo Valeria Conti Nibali Giovanna D’Angelo |
| author_sort | Sara Anselmo |
| collection | DOAJ |
| description | Lipid rafts are dynamic microdomains in the membrane, rich in cholesterol and sphingolipids, that are critical for biological processes like cell signalling, membrane trafficking, and protein organization. Their essential role is claimed in both physiological and pathological conditions, including cancer, neurodegenerative diseases, and viral infections, making them a key area of research. Fluorescence-based approaches, including super-resolution fluorescence microscopy techniques, enable precise analysis of the organization, dynamics, and interactions of these microdomains, thanks also to the innovative design of appropriate fluorescent probes. Moreover, these non-invasive approaches allow for the study of live cells, facilitating the collection of quantitative data under physiologically relevant conditions. This review synthesizes the latest insights into the role of lipid rafts in biological and pathological processes and underscores how fluorescence techniques have advanced our understanding of these critical microdomains. The findings emphasize the pivotal role of lipid rafts in health and disease, providing a foundation for future research and potential therapeutic interventions. |
| format | Article |
| id | doaj-art-20c09c6c1c6f4565bf0ea1e9ed9a629e |
| institution | Kabale University |
| issn | 2077-0375 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj-art-20c09c6c1c6f4565bf0ea1e9ed9a629e2025-01-24T13:40:59ZengMDPI AGMembranes2077-03752025-01-01151610.3390/membranes15010006Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?Sara Anselmo0Elisa Bonaccorso1Chiara Gangemi2Giuseppe Sancataldo3Valeria Conti Nibali4Giovanna D’Angelo5Department of Physics and Chemistry-Emilio Segré, University of Palermo, Viale delle Scienze, 90128 Palermo, ItalyDepartment of Mathematics, Computer Science, Physics and Earth Science, University of Messina, Viale Stagno D’Alcontres 31, 98166 Messina, ItalyDepartment of Mathematics, Computer Science, Physics and Earth Science, University of Messina, Viale Stagno D’Alcontres 31, 98166 Messina, ItalyDepartment of Physics and Chemistry-Emilio Segré, University of Palermo, Viale delle Scienze, 90128 Palermo, ItalyDepartment of Mathematics, Computer Science, Physics and Earth Science, University of Messina, Viale Stagno D’Alcontres 31, 98166 Messina, ItalyDepartment of Mathematics, Computer Science, Physics and Earth Science, University of Messina, Viale Stagno D’Alcontres 31, 98166 Messina, ItalyLipid rafts are dynamic microdomains in the membrane, rich in cholesterol and sphingolipids, that are critical for biological processes like cell signalling, membrane trafficking, and protein organization. Their essential role is claimed in both physiological and pathological conditions, including cancer, neurodegenerative diseases, and viral infections, making them a key area of research. Fluorescence-based approaches, including super-resolution fluorescence microscopy techniques, enable precise analysis of the organization, dynamics, and interactions of these microdomains, thanks also to the innovative design of appropriate fluorescent probes. Moreover, these non-invasive approaches allow for the study of live cells, facilitating the collection of quantitative data under physiologically relevant conditions. This review synthesizes the latest insights into the role of lipid rafts in biological and pathological processes and underscores how fluorescence techniques have advanced our understanding of these critical microdomains. The findings emphasize the pivotal role of lipid rafts in health and disease, providing a foundation for future research and potential therapeutic interventions.https://www.mdpi.com/2077-0375/15/1/6lipid raftsliquid-ordered phasefluorescent probessuper resolutionneurodegenerative diseaseinfections |
| spellingShingle | Sara Anselmo Elisa Bonaccorso Chiara Gangemi Giuseppe Sancataldo Valeria Conti Nibali Giovanna D’Angelo Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques? Membranes lipid rafts liquid-ordered phase fluorescent probes super resolution neurodegenerative disease infections |
| title | Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques? |
| title_full | Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques? |
| title_fullStr | Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques? |
| title_full_unstemmed | Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques? |
| title_short | Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques? |
| title_sort | lipid rafts in signalling diseases and infections what can be learned from fluorescence techniques |
| topic | lipid rafts liquid-ordered phase fluorescent probes super resolution neurodegenerative disease infections |
| url | https://www.mdpi.com/2077-0375/15/1/6 |
| work_keys_str_mv | AT saraanselmo lipidraftsinsignallingdiseasesandinfectionswhatcanbelearnedfromfluorescencetechniques AT elisabonaccorso lipidraftsinsignallingdiseasesandinfectionswhatcanbelearnedfromfluorescencetechniques AT chiaragangemi lipidraftsinsignallingdiseasesandinfectionswhatcanbelearnedfromfluorescencetechniques AT giuseppesancataldo lipidraftsinsignallingdiseasesandinfectionswhatcanbelearnedfromfluorescencetechniques AT valeriacontinibali lipidraftsinsignallingdiseasesandinfectionswhatcanbelearnedfromfluorescencetechniques AT giovannadangelo lipidraftsinsignallingdiseasesandinfectionswhatcanbelearnedfromfluorescencetechniques |