Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations
A simple, fast and precise reverse phase high performance liquid chromatographic method is developed for the simultaneous determination of aceclofenac, paracetamol and tizanidine. Chromatographic separation of the three drugs were performed on a hypersil C18 column (250 mm x 4.6 mm, 5 µm) as station...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/323410 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564453878530048 |
|---|---|
| author | V. V. Vaidya G. R. Singh M. P. Choukekar M. B. Kekare |
| author_facet | V. V. Vaidya G. R. Singh M. P. Choukekar M. B. Kekare |
| author_sort | V. V. Vaidya |
| collection | DOAJ |
| description | A simple, fast and precise reverse phase high performance liquid chromatographic method is developed for the simultaneous determination of aceclofenac, paracetamol and tizanidine. Chromatographic separation of the three drugs were performed on a hypersil C18 column (250 mm x 4.6 mm, 5 µm) as stationary phase with a mobile phase comprising of mix phosphate buffer pH 7.0: acetonitrile (40:60 v/v), at a flow rate of 0.7 mL min-1 and UV detection at 230 nm. The proposed method was validated for linearity, accuracy, precision, LOD, LOQ. Linearity, accuracy and precision were found to be acceptable over the ranges of 100-300 µg mL-1 for aceclofenac, 500-1500 µg mL-1 for paracetamol and 2-6 µg mL-1 for tizanidine HCl equivalent to tizanidine. It can be conveniently adopted for routine quality control analysis. |
| format | Article |
| id | doaj-art-20b58c6a94a6438f8bf27a1c58a52454 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-20b58c6a94a6438f8bf27a1c58a524542025-02-03T01:10:51ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017126026410.1155/2010/323410Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical PreparationsV. V. Vaidya0G. R. Singh1M. P. Choukekar2M. B. Kekare3Department of Chemistry, S.P.Mandali’s Ramnarain Ruia College, Matunga, Mumbai-400019, IndiaDepartment of Chemistry, S.P.Mandali’s Ramnarain Ruia College, Matunga, Mumbai-400019, IndiaDepartment of Chemistry.Kirti M. Dongursee College, Dadar, Mumbai-400028, IndiaDepartment of Chemistry.Kirti M. Dongursee College, Dadar, Mumbai-400028, IndiaA simple, fast and precise reverse phase high performance liquid chromatographic method is developed for the simultaneous determination of aceclofenac, paracetamol and tizanidine. Chromatographic separation of the three drugs were performed on a hypersil C18 column (250 mm x 4.6 mm, 5 µm) as stationary phase with a mobile phase comprising of mix phosphate buffer pH 7.0: acetonitrile (40:60 v/v), at a flow rate of 0.7 mL min-1 and UV detection at 230 nm. The proposed method was validated for linearity, accuracy, precision, LOD, LOQ. Linearity, accuracy and precision were found to be acceptable over the ranges of 100-300 µg mL-1 for aceclofenac, 500-1500 µg mL-1 for paracetamol and 2-6 µg mL-1 for tizanidine HCl equivalent to tizanidine. It can be conveniently adopted for routine quality control analysis.http://dx.doi.org/10.1155/2010/323410 |
| spellingShingle | V. V. Vaidya G. R. Singh M. P. Choukekar M. B. Kekare Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations E-Journal of Chemistry |
| title | Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations |
| title_full | Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations |
| title_fullStr | Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations |
| title_full_unstemmed | Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations |
| title_short | Simultaneous RP HPLC Determination of Aceclofenac, Paracetamol and Tizanidine in Pharmaceutical Preparations |
| title_sort | simultaneous rp hplc determination of aceclofenac paracetamol and tizanidine in pharmaceutical preparations |
| url | http://dx.doi.org/10.1155/2010/323410 |
| work_keys_str_mv | AT vvvaidya simultaneousrphplcdeterminationofaceclofenacparacetamolandtizanidineinpharmaceuticalpreparations AT grsingh simultaneousrphplcdeterminationofaceclofenacparacetamolandtizanidineinpharmaceuticalpreparations AT mpchoukekar simultaneousrphplcdeterminationofaceclofenacparacetamolandtizanidineinpharmaceuticalpreparations AT mbkekare simultaneousrphplcdeterminationofaceclofenacparacetamolandtizanidineinpharmaceuticalpreparations |