A Validated RP-HPLC Method for the Estimation of Quetiapine in Bulk and Pharmaceutical Formulations
A new, simple, specific, sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of quetiapine in bulk and pharmaceutical formulations. Quetiapine was chromatographed on a reverse phase C18 Waters column (75x4.6 mm I.D., particle size 3.5 μm) in a mobile phase consisti...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/105963 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563341180010496 |
|---|---|
| author | D. Suneetha A. Lakshmana Rao |
| author_facet | D. Suneetha A. Lakshmana Rao |
| author_sort | D. Suneetha |
| collection | DOAJ |
| description | A new, simple, specific, sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of quetiapine in bulk and pharmaceutical formulations. Quetiapine was chromatographed on a reverse phase C18 Waters column (75x4.6 mm I.D., particle size 3.5 μm) in a mobile phase consisting of phosphate buffer (pH 3.0 adjusted with orthophosphoric acid) and acetonitrile in the ratio 40:60 v/v. The mobile phase was pumped at a flow rate of 0.8 mL/min with detection at 291 nm. The detector response was linear in the concentration of 20-120 μg/mL. The limit of detection and limit of quantitation was found to be 0.2 and 0.75 μg/mL, respectively. The intra and inter day variation was found to be less than 1%. The mean recovery of the drug from the solution was 99%. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of quetiapine in bulk and pharmaceutical formulations. |
| format | Article |
| id | doaj-art-209f3f5e3e6540dba54b8512697a76f9 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-209f3f5e3e6540dba54b8512697a76f92025-02-03T01:20:18ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017S1S261S26610.1155/2010/105963A Validated RP-HPLC Method for the Estimation of Quetiapine in Bulk and Pharmaceutical FormulationsD. Suneetha0A. Lakshmana Rao1Department of Pharmaceutical Analysis, Shri Vishnu College of Pharmacy Vishnupur, Bhimavaram-534 202, A.P., IndiaDepartment of Pharmaceutical Analysis, Shri Vishnu College of Pharmacy Vishnupur, Bhimavaram-534 202, A.P., IndiaA new, simple, specific, sensitive, rapid, accurate and precise RP-HPLC method was developed for the estimation of quetiapine in bulk and pharmaceutical formulations. Quetiapine was chromatographed on a reverse phase C18 Waters column (75x4.6 mm I.D., particle size 3.5 μm) in a mobile phase consisting of phosphate buffer (pH 3.0 adjusted with orthophosphoric acid) and acetonitrile in the ratio 40:60 v/v. The mobile phase was pumped at a flow rate of 0.8 mL/min with detection at 291 nm. The detector response was linear in the concentration of 20-120 μg/mL. The limit of detection and limit of quantitation was found to be 0.2 and 0.75 μg/mL, respectively. The intra and inter day variation was found to be less than 1%. The mean recovery of the drug from the solution was 99%. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of quetiapine in bulk and pharmaceutical formulations.http://dx.doi.org/10.1155/2010/105963 |
| spellingShingle | D. Suneetha A. Lakshmana Rao A Validated RP-HPLC Method for the Estimation of Quetiapine in Bulk and Pharmaceutical Formulations E-Journal of Chemistry |
| title | A Validated RP-HPLC Method for the Estimation of Quetiapine in Bulk and Pharmaceutical Formulations |
| title_full | A Validated RP-HPLC Method for the Estimation of Quetiapine in Bulk and Pharmaceutical Formulations |
| title_fullStr | A Validated RP-HPLC Method for the Estimation of Quetiapine in Bulk and Pharmaceutical Formulations |
| title_full_unstemmed | A Validated RP-HPLC Method for the Estimation of Quetiapine in Bulk and Pharmaceutical Formulations |
| title_short | A Validated RP-HPLC Method for the Estimation of Quetiapine in Bulk and Pharmaceutical Formulations |
| title_sort | validated rp hplc method for the estimation of quetiapine in bulk and pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2010/105963 |
| work_keys_str_mv | AT dsuneetha avalidatedrphplcmethodfortheestimationofquetiapineinbulkandpharmaceuticalformulations AT alakshmanarao avalidatedrphplcmethodfortheestimationofquetiapineinbulkandpharmaceuticalformulations AT dsuneetha validatedrphplcmethodfortheestimationofquetiapineinbulkandpharmaceuticalformulations AT alakshmanarao validatedrphplcmethodfortheestimationofquetiapineinbulkandpharmaceuticalformulations |