The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood
Background. Matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) contribute to the inflammatory cascade in the cerebrospinal fluid (CSF) during bacterial meningitis. We determined levels of MPO, MMP-8, MMP-9, and tissue inhibitor of metalloproteinase- (TIMP-) 1 in the CSF of children with bact...
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| Format: | Article |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/7436932 |
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| author | Okko Savonius Irmeli Roine Saeed Alassiri Taina Tervahartiala Otto Helve Josefina Fernández Heikki Peltola Timo Sorsa Tuula Pelkonen |
| author_facet | Okko Savonius Irmeli Roine Saeed Alassiri Taina Tervahartiala Otto Helve Josefina Fernández Heikki Peltola Timo Sorsa Tuula Pelkonen |
| author_sort | Okko Savonius |
| collection | DOAJ |
| description | Background. Matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) contribute to the inflammatory cascade in the cerebrospinal fluid (CSF) during bacterial meningitis. We determined levels of MPO, MMP-8, MMP-9, and tissue inhibitor of metalloproteinase- (TIMP-) 1 in the CSF of children with bacterial meningitis and investigated how these inflammatory mediators relate to each other and to the disease outcomes. Methods. Clinical data and the diagnostic CSF samples from 245 children (median age eight months) with bacterial meningitis were obtained from a clinical trial in Latin America in 1996–2003. MMP-9 levels in the CSF were assessed by zymography, while MMP-8, MPO, and TIMP-1 concentrations were determined with immunofluorometric and enzyme-linked immunosorbent assays. Results. MPO correlated positively with MMP-8 (rho 0.496, P<0.001) and MMP-9 (rho 0.153, P=0.02) but negatively with TIMP-1 (rho -0.361, P<0.001). MMP-8 emerged as the best predictor of disease outcomes: a CSF MMP-8 concentration above the median increased the odds of death 4.9-fold (95% confidence interval 1.8–12.9). Conclusions. CSF MMP-8 presented as an attractive prognostic marker in children with bacterial meningitis. |
| format | Article |
| id | doaj-art-2053feb66b104fe596e16512943bf9f9 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2053feb66b104fe596e16512943bf9f92025-02-03T05:45:35ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/74369327436932The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of ChildhoodOkko Savonius0Irmeli Roine1Saeed Alassiri2Taina Tervahartiala3Otto Helve4Josefina Fernández5Heikki Peltola6Timo Sorsa7Tuula Pelkonen8Children’s Hospital, Pediatric Research Center, University of Helsinki, Helsinki University Hospital, Helsinki, FinlandFaculty of Medicine, University Diego Portales, Santiago, ChileDepartment of Oral and Maxillofacial Diseases, Institute of Dentistry, Helsinki University Hospital and the University of Helsinki, Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, Institute of Dentistry, Helsinki University Hospital and the University of Helsinki, Helsinki, FinlandChildren’s Hospital, Pediatric Research Center, University of Helsinki, Helsinki University Hospital, Helsinki, FinlandClínica Infantil Dr. Robert Reid Cabral, Santo Domingo, Dominican RepublicChildren’s Hospital, Pediatric Research Center, University of Helsinki, Helsinki University Hospital, Helsinki, FinlandDepartment of Oral and Maxillofacial Diseases, Institute of Dentistry, Helsinki University Hospital and the University of Helsinki, Helsinki, FinlandChildren’s Hospital, Pediatric Research Center, University of Helsinki, Helsinki University Hospital, Helsinki, FinlandBackground. Matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) contribute to the inflammatory cascade in the cerebrospinal fluid (CSF) during bacterial meningitis. We determined levels of MPO, MMP-8, MMP-9, and tissue inhibitor of metalloproteinase- (TIMP-) 1 in the CSF of children with bacterial meningitis and investigated how these inflammatory mediators relate to each other and to the disease outcomes. Methods. Clinical data and the diagnostic CSF samples from 245 children (median age eight months) with bacterial meningitis were obtained from a clinical trial in Latin America in 1996–2003. MMP-9 levels in the CSF were assessed by zymography, while MMP-8, MPO, and TIMP-1 concentrations were determined with immunofluorometric and enzyme-linked immunosorbent assays. Results. MPO correlated positively with MMP-8 (rho 0.496, P<0.001) and MMP-9 (rho 0.153, P=0.02) but negatively with TIMP-1 (rho -0.361, P<0.001). MMP-8 emerged as the best predictor of disease outcomes: a CSF MMP-8 concentration above the median increased the odds of death 4.9-fold (95% confidence interval 1.8–12.9). Conclusions. CSF MMP-8 presented as an attractive prognostic marker in children with bacterial meningitis.http://dx.doi.org/10.1155/2019/7436932 |
| spellingShingle | Okko Savonius Irmeli Roine Saeed Alassiri Taina Tervahartiala Otto Helve Josefina Fernández Heikki Peltola Timo Sorsa Tuula Pelkonen The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood Mediators of Inflammation |
| title | The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood |
| title_full | The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood |
| title_fullStr | The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood |
| title_full_unstemmed | The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood |
| title_short | The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood |
| title_sort | potential role of matrix metalloproteinases 8 and 9 and myeloperoxidase in predicting outcomes of bacterial meningitis of childhood |
| url | http://dx.doi.org/10.1155/2019/7436932 |
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