Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations
Genetic diversity of T. gondii is a concern of many studies, due to the biological and epidemiological diversity of this parasite. The present study examined sequence variation in rhoptry protein 17 (ROP17) gene among T. gondii isolates from different hosts and geographical regions. The rop17 gene w...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/349325 |
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| author | Nian-Zhang Zhang Ying Xu Si-Yang Huang Dong-Hui Zhou Rui-Ai Wang Xing-Quan Zhu |
| author_facet | Nian-Zhang Zhang Ying Xu Si-Yang Huang Dong-Hui Zhou Rui-Ai Wang Xing-Quan Zhu |
| author_sort | Nian-Zhang Zhang |
| collection | DOAJ |
| description | Genetic diversity of T. gondii is a concern of many studies, due to the biological and epidemiological diversity of this parasite. The present study examined sequence variation in rhoptry protein 17 (ROP17) gene among T. gondii isolates from different hosts and geographical regions. The rop17 gene was amplified and sequenced from 10 T. gondii strains, and phylogenetic relationship among these T. gondii strains was reconstructed using maximum parsimony (MP), neighbor-joining (NJ), and maximum likelihood (ML) analyses. The partial rop17 gene sequences were 1375 bp in length and A+T contents varied from 49.45% to 50.11% among all examined T. gondii strains. Sequence analysis identified 33 variable nucleotide positions (2.1%), 16 of which were identified as transitions. Phylogeny reconstruction based on rop17 gene data revealed two major clusters which could readily distinguish Type I and Type II strains. Analyses of sequence variations in nucleotides and amino acids among these strains revealed high ratio of nonsynonymous to synonymous polymorphisms (>1), indicating that rop17 shows signs of positive selection. This study demonstrated the existence of slightly high sequence variability in the rop17 gene sequences among T. gondii strains from different hosts and geographical regions, suggesting that rop17 gene may represent a new genetic marker for population genetic studies of T. gondii isolates. |
| format | Article |
| id | doaj-art-20516a0f0d5343bc8d0160f1382611b3 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-20516a0f0d5343bc8d0160f1382611b32025-02-03T01:22:25ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/349325349325Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical LocationsNian-Zhang Zhang0Ying Xu1Si-Yang Huang2Dong-Hui Zhou3Rui-Ai Wang4Xing-Quan Zhu5State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, ChinaGenetic diversity of T. gondii is a concern of many studies, due to the biological and epidemiological diversity of this parasite. The present study examined sequence variation in rhoptry protein 17 (ROP17) gene among T. gondii isolates from different hosts and geographical regions. The rop17 gene was amplified and sequenced from 10 T. gondii strains, and phylogenetic relationship among these T. gondii strains was reconstructed using maximum parsimony (MP), neighbor-joining (NJ), and maximum likelihood (ML) analyses. The partial rop17 gene sequences were 1375 bp in length and A+T contents varied from 49.45% to 50.11% among all examined T. gondii strains. Sequence analysis identified 33 variable nucleotide positions (2.1%), 16 of which were identified as transitions. Phylogeny reconstruction based on rop17 gene data revealed two major clusters which could readily distinguish Type I and Type II strains. Analyses of sequence variations in nucleotides and amino acids among these strains revealed high ratio of nonsynonymous to synonymous polymorphisms (>1), indicating that rop17 shows signs of positive selection. This study demonstrated the existence of slightly high sequence variability in the rop17 gene sequences among T. gondii strains from different hosts and geographical regions, suggesting that rop17 gene may represent a new genetic marker for population genetic studies of T. gondii isolates.http://dx.doi.org/10.1155/2014/349325 |
| spellingShingle | Nian-Zhang Zhang Ying Xu Si-Yang Huang Dong-Hui Zhou Rui-Ai Wang Xing-Quan Zhu Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations The Scientific World Journal |
| title | Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations |
| title_full | Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations |
| title_fullStr | Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations |
| title_full_unstemmed | Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations |
| title_short | Sequence Variation in Toxoplasma gondii rop17 Gene among Strains from Different Hosts and Geographical Locations |
| title_sort | sequence variation in toxoplasma gondii rop17 gene among strains from different hosts and geographical locations |
| url | http://dx.doi.org/10.1155/2014/349325 |
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