Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells
The effects of acute exposure to high glucose levels as experienced by glomerular mesangial cells in postprandial conditions and states such as in prediabetes were investigated using proteomic methods. Two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/3537863 |
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| _version_ | 1832553730536374272 |
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| author | Michelle T. Barati James C. Gould Sarah A. Salyer Susan Isaacs Daniel W. Wilkey Michael L. Merchant |
| author_facet | Michelle T. Barati James C. Gould Sarah A. Salyer Susan Isaacs Daniel W. Wilkey Michael L. Merchant |
| author_sort | Michelle T. Barati |
| collection | DOAJ |
| description | The effects of acute exposure to high glucose levels as experienced by glomerular mesangial cells in postprandial conditions and states such as in prediabetes were investigated using proteomic methods. Two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry methods were used to identify protein expression patterns in immortalized rat mesangial cells altered by 2 h high glucose (HG) growth conditions as compared to isoosmotic/normal glucose control (NG⁎) conditions. Unique protein expression changes at 2 h HG treatment were measured for 51 protein spots. These proteins could be broadly grouped into two categories: (1) proteins involved in cell survival/cell signaling and (2) proteins involved in stress response. Immunoblot experiments for a protein belonging to both categories, prohibitin (PHB), supported a trend for increased total expression as well as significant increases in an acidic PHB isoform. Additional studies confirmed the regulation of proteasomal subunit alpha-type 2 and the endoplasmic reticulum chaperone and oxidoreductase PDI (protein disulfide isomerase), suggesting altered ER protein folding capacity and proteasomal function in response to acute HG. We conclude that short term high glucose induces subtle changes in protein abundances suggesting posttranslational modifications and regulation of pathways involved in proteostasis. |
| format | Article |
| id | doaj-art-1ff428d0f6c34f88a294c831293a3369 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-1ff428d0f6c34f88a294c831293a33692025-02-03T05:53:24ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/35378633537863Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial CellsMichelle T. Barati0James C. Gould1Sarah A. Salyer2Susan Isaacs3Daniel W. Wilkey4Michael L. Merchant5Kidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USAKidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USAKidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USAKidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USAKidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USAKidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USAThe effects of acute exposure to high glucose levels as experienced by glomerular mesangial cells in postprandial conditions and states such as in prediabetes were investigated using proteomic methods. Two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry methods were used to identify protein expression patterns in immortalized rat mesangial cells altered by 2 h high glucose (HG) growth conditions as compared to isoosmotic/normal glucose control (NG⁎) conditions. Unique protein expression changes at 2 h HG treatment were measured for 51 protein spots. These proteins could be broadly grouped into two categories: (1) proteins involved in cell survival/cell signaling and (2) proteins involved in stress response. Immunoblot experiments for a protein belonging to both categories, prohibitin (PHB), supported a trend for increased total expression as well as significant increases in an acidic PHB isoform. Additional studies confirmed the regulation of proteasomal subunit alpha-type 2 and the endoplasmic reticulum chaperone and oxidoreductase PDI (protein disulfide isomerase), suggesting altered ER protein folding capacity and proteasomal function in response to acute HG. We conclude that short term high glucose induces subtle changes in protein abundances suggesting posttranslational modifications and regulation of pathways involved in proteostasis.http://dx.doi.org/10.1155/2016/3537863 |
| spellingShingle | Michelle T. Barati James C. Gould Sarah A. Salyer Susan Isaacs Daniel W. Wilkey Michael L. Merchant Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells Journal of Diabetes Research |
| title | Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells |
| title_full | Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells |
| title_fullStr | Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells |
| title_full_unstemmed | Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells |
| title_short | Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells |
| title_sort | influence of acute high glucose on protein abundance changes in murine glomerular mesangial cells |
| url | http://dx.doi.org/10.1155/2016/3537863 |
| work_keys_str_mv | AT michelletbarati influenceofacutehighglucoseonproteinabundancechangesinmurineglomerularmesangialcells AT jamescgould influenceofacutehighglucoseonproteinabundancechangesinmurineglomerularmesangialcells AT sarahasalyer influenceofacutehighglucoseonproteinabundancechangesinmurineglomerularmesangialcells AT susanisaacs influenceofacutehighglucoseonproteinabundancechangesinmurineglomerularmesangialcells AT danielwwilkey influenceofacutehighglucoseonproteinabundancechangesinmurineglomerularmesangialcells AT michaellmerchant influenceofacutehighglucoseonproteinabundancechangesinmurineglomerularmesangialcells |