Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma
A simple, fast, and cost-effective LC-MS/MS method for quantification of rifampicin in human plasma was developed and fully validated. The plasma samples containing rifampicin and isotopically labelled internal standard rifampicin D8, were cleaned up using a Captiva ND Lipids filtration plate. Chrom...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/4848236 |
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| author | Žane Temova Rakuša Robert Roškar Anita Klančar Andrejc Tina Trdan Lušin Nataša Faganeli Iztok Grabnar Aleš Mrhar Albin Kristl Jurij Trontelj |
| author_facet | Žane Temova Rakuša Robert Roškar Anita Klančar Andrejc Tina Trdan Lušin Nataša Faganeli Iztok Grabnar Aleš Mrhar Albin Kristl Jurij Trontelj |
| author_sort | Žane Temova Rakuša |
| collection | DOAJ |
| description | A simple, fast, and cost-effective LC-MS/MS method for quantification of rifampicin in human plasma was developed and fully validated. The plasma samples containing rifampicin and isotopically labelled internal standard rifampicin D8, were cleaned up using a Captiva ND Lipids filtration plate. Chromatographic separation was achieved on an 1290 Infinity liquid chromatograph coupled to 6460 Triple Quadrupole operated in positive mode on a core-shell Kinetex C18 column (50 × 2.1 mm, 2.6 μm) by gradient elution using 0.1% formic acid in water and acetonitrile as a mobile phase. The proposed method is the fastest method published by now, both in terms of sample preparation (approximately one minute per sample) and chromatographic analysis (total run time 2.4 min). Another key benefit is the outstanding sensitivity and wide analytical range (5-40000 μg/L) with good linearity, accuracy, and precision. The method showed almost complete recovery (92%) and absence of any significant matrix effect as demonstrated by uniform responses from QC samples prepared in blood plasma from 6 volunteers (RSD <5%). The proposed method was successfully applied to rifampicin quantification in 340 patients’ plasma samples, thus demonstrating its suitability for both therapeutic drug monitoring and pharmacokinetic analysis. |
| format | Article |
| id | doaj-art-1fc16eb82b664d9c940255043dc92af3 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-1fc16eb82b664d9c940255043dc92af32025-02-03T01:27:12ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792019-01-01201910.1155/2019/48482364848236Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human PlasmaŽane Temova Rakuša0Robert Roškar1Anita Klančar Andrejc2Tina Trdan Lušin3Nataša Faganeli4Iztok Grabnar5Aleš Mrhar6Albin Kristl7Jurij Trontelj8University of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, SloveniaA simple, fast, and cost-effective LC-MS/MS method for quantification of rifampicin in human plasma was developed and fully validated. The plasma samples containing rifampicin and isotopically labelled internal standard rifampicin D8, were cleaned up using a Captiva ND Lipids filtration plate. Chromatographic separation was achieved on an 1290 Infinity liquid chromatograph coupled to 6460 Triple Quadrupole operated in positive mode on a core-shell Kinetex C18 column (50 × 2.1 mm, 2.6 μm) by gradient elution using 0.1% formic acid in water and acetonitrile as a mobile phase. The proposed method is the fastest method published by now, both in terms of sample preparation (approximately one minute per sample) and chromatographic analysis (total run time 2.4 min). Another key benefit is the outstanding sensitivity and wide analytical range (5-40000 μg/L) with good linearity, accuracy, and precision. The method showed almost complete recovery (92%) and absence of any significant matrix effect as demonstrated by uniform responses from QC samples prepared in blood plasma from 6 volunteers (RSD <5%). The proposed method was successfully applied to rifampicin quantification in 340 patients’ plasma samples, thus demonstrating its suitability for both therapeutic drug monitoring and pharmacokinetic analysis.http://dx.doi.org/10.1155/2019/4848236 |
| spellingShingle | Žane Temova Rakuša Robert Roškar Anita Klančar Andrejc Tina Trdan Lušin Nataša Faganeli Iztok Grabnar Aleš Mrhar Albin Kristl Jurij Trontelj Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma International Journal of Analytical Chemistry |
| title | Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma |
| title_full | Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma |
| title_fullStr | Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma |
| title_full_unstemmed | Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma |
| title_short | Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma |
| title_sort | fast and simple lc ms ms method for rifampicin quantification in human plasma |
| url | http://dx.doi.org/10.1155/2019/4848236 |
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