Intraindividual variation in histone acetylation and its impact on autoimmune thyroid diseases
Autoimmune thyroid diseases (AITDs), such as Graves’ disease (GD) and Hashimoto’s disease (HD), are organ-specific autoimmune diseases. Histone acetylation, especially that of histone H3, is an epigenetic mechanism that regulates gene expression and is associated with the development of autoimmune d...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Japan Endocrine Society
2023-12-01
|
| Series: | Endocrine Journal |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/endocrj/70/12/70_EJ23-0204/_html/-char/en |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591680656637952 |
|---|---|
| author | Emi Haga Naoya Inoue Yoshinori Iwatani Yuya Arakawa Emi Morita Hidemi Hashimoto Yusuke Noguchi Yoh Hidaka Mikio Watanabe |
| author_facet | Emi Haga Naoya Inoue Yoshinori Iwatani Yuya Arakawa Emi Morita Hidemi Hashimoto Yusuke Noguchi Yoh Hidaka Mikio Watanabe |
| author_sort | Emi Haga |
| collection | DOAJ |
| description | Autoimmune thyroid diseases (AITDs), such as Graves’ disease (GD) and Hashimoto’s disease (HD), are organ-specific autoimmune diseases. Histone acetylation, especially that of histone H3, is an epigenetic mechanism that regulates gene expression and is associated with the development of autoimmune diseases. However, physiological variations in histone acetylation are not yet clear, and we believe that physiological variations should be examined prior to analysis of the role of histone H3 in the pathogenesis of AITDs. In this study, we analyzed histone H3 acetylation levels in peripheral blood mononuclear cells (PBMCs) using a histone H3 total acetylation detection fast kit. Blood samples were collected before meals, between 8:30–9:00 am, daily for 10 weeks to evaluate the daily variation. At 4 days, blood was also collected before meals three times a day (at 8:30–9:00, 12:30–13:00, and 16:30–17:00) to evaluate circadian variation. Then, histone H3 acetylation levels were evaluated in AITD patients to clarify the association with the pathogenesis of AITD. Although we could not find a common pattern of circadian variance, we observed daily variation in histone H3 acetylation levels, and their coefficient of variances (CVs) were approximately 48.3%. Then, we found that histone H3 acetylation levels were significantly lower in GD and HD patients than in control subjects and these differences were larger than the daily variation in histone acetylation. In conclusion, histone H3 acetylation levels were associated with the development of AITD, even allowing for daily variation. |
| format | Article |
| id | doaj-art-1fafb4da2f48492cbf56e82462671c7e |
| institution | Kabale University |
| issn | 1348-4540 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | The Japan Endocrine Society |
| record_format | Article |
| series | Endocrine Journal |
| spelling | doaj-art-1fafb4da2f48492cbf56e82462671c7e2025-01-22T06:25:09ZengThe Japan Endocrine SocietyEndocrine Journal1348-45402023-12-0170121169117410.1507/endocrj.EJ23-0204endocrjIntraindividual variation in histone acetylation and its impact on autoimmune thyroid diseasesEmi Haga0Naoya Inoue1Yoshinori Iwatani2Yuya Arakawa3Emi Morita4Hidemi Hashimoto5Yusuke Noguchi6Yoh Hidaka7Mikio Watanabe8Department of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanLaboratory for Clinical Investigation, Osaka University Hospital, Osaka 565-0871, JapanDepartment of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka 565-0871, JapanAutoimmune thyroid diseases (AITDs), such as Graves’ disease (GD) and Hashimoto’s disease (HD), are organ-specific autoimmune diseases. Histone acetylation, especially that of histone H3, is an epigenetic mechanism that regulates gene expression and is associated with the development of autoimmune diseases. However, physiological variations in histone acetylation are not yet clear, and we believe that physiological variations should be examined prior to analysis of the role of histone H3 in the pathogenesis of AITDs. In this study, we analyzed histone H3 acetylation levels in peripheral blood mononuclear cells (PBMCs) using a histone H3 total acetylation detection fast kit. Blood samples were collected before meals, between 8:30–9:00 am, daily for 10 weeks to evaluate the daily variation. At 4 days, blood was also collected before meals three times a day (at 8:30–9:00, 12:30–13:00, and 16:30–17:00) to evaluate circadian variation. Then, histone H3 acetylation levels were evaluated in AITD patients to clarify the association with the pathogenesis of AITD. Although we could not find a common pattern of circadian variance, we observed daily variation in histone H3 acetylation levels, and their coefficient of variances (CVs) were approximately 48.3%. Then, we found that histone H3 acetylation levels were significantly lower in GD and HD patients than in control subjects and these differences were larger than the daily variation in histone acetylation. In conclusion, histone H3 acetylation levels were associated with the development of AITD, even allowing for daily variation.https://www.jstage.jst.go.jp/article/endocrj/70/12/70_EJ23-0204/_html/-char/enacetylationdaily variationcircadian variationautoimmune thyroid disease |
| spellingShingle | Emi Haga Naoya Inoue Yoshinori Iwatani Yuya Arakawa Emi Morita Hidemi Hashimoto Yusuke Noguchi Yoh Hidaka Mikio Watanabe Intraindividual variation in histone acetylation and its impact on autoimmune thyroid diseases Endocrine Journal acetylation daily variation circadian variation autoimmune thyroid disease |
| title | Intraindividual variation in histone acetylation and its impact on autoimmune thyroid diseases |
| title_full | Intraindividual variation in histone acetylation and its impact on autoimmune thyroid diseases |
| title_fullStr | Intraindividual variation in histone acetylation and its impact on autoimmune thyroid diseases |
| title_full_unstemmed | Intraindividual variation in histone acetylation and its impact on autoimmune thyroid diseases |
| title_short | Intraindividual variation in histone acetylation and its impact on autoimmune thyroid diseases |
| title_sort | intraindividual variation in histone acetylation and its impact on autoimmune thyroid diseases |
| topic | acetylation daily variation circadian variation autoimmune thyroid disease |
| url | https://www.jstage.jst.go.jp/article/endocrj/70/12/70_EJ23-0204/_html/-char/en |
| work_keys_str_mv | AT emihaga intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases AT naoyainoue intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases AT yoshinoriiwatani intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases AT yuyaarakawa intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases AT emimorita intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases AT hidemihashimoto intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases AT yusukenoguchi intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases AT yohhidaka intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases AT mikiowatanabe intraindividualvariationinhistoneacetylationanditsimpactonautoimmunethyroiddiseases |