Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis
Introduction. Ankylosing spondylitis (AS) and psoriatic arthritis (PsA) are classified as spondyloarthritis (SpA), a group of inflammatory rheumatic diseases with complex genetic etiology. Toll-like receptors (TLRs) have an important role in the mechanism of innate immunity and may influence inflamm...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/1492092 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548936284372992 |
|---|---|
| author | Camila F. Oliveira-Toré Amarilis G. Moraes Gabriela F. Martinez Janisleya S. F. Neves Luciana C. Macedo Marco A. Rocha-Loures Marília G. Quirino Hugo V. Alves Ana M. Sell Jeane E. L. Visentainer |
| author_facet | Camila F. Oliveira-Toré Amarilis G. Moraes Gabriela F. Martinez Janisleya S. F. Neves Luciana C. Macedo Marco A. Rocha-Loures Marília G. Quirino Hugo V. Alves Ana M. Sell Jeane E. L. Visentainer |
| author_sort | Camila F. Oliveira-Toré |
| collection | DOAJ |
| description | Introduction. Ankylosing spondylitis (AS) and psoriatic arthritis (PsA) are classified as spondyloarthritis (SpA), a group of inflammatory rheumatic diseases with complex genetic etiology. Toll-like receptors (TLRs) have an important role in the mechanism of innate immunity and may influence inflammatory responses. Polymorphisms in TLR genes that lead to changes in these receptors or that interfere with the transcription rates of mRNA TLR may be involved in the chronic inflammatory immune response observed in SpA. Currently, there is a lack of studies associating genetic polymorphisms in TLRs and SpA. Objective. Therefore, this case-control study is aimed at analyzing the influence of the respective SNPs on TLR2 rs5743708, TLR6 rs5743810, and TLR9 rs5743836 and rs187084 in the immunopathogenesis of SpA. Methods. The polymorphisms genotyped by PCR-RFLP were TLR2 rs5743708, TLR6 rs5743810, and TLR9 rs5743836 and rs187084. The HLA-B∗27 was performed by PCR-SSP. Results. Logistic regression analysis showed a strong association between SNPs in TLR2 and TLR9 and susceptibility to SpA (OR=12.56; CI=6.5-25.9 and OR=1.62; CI=1.20-2.21, respectively). No association was observed among HLA-B∗27 and TLR polymorphisms (p=0.72), nor among BASDAI and TLR polymorphisms (p=0.85). Discussion. Our findings suggest that polymorphisms in TLR2 and TLR9 genes may contribute to the immunopathogenesis of the SpA. The rs187084, rs5743836, and rs5743708 polymorphisms were associated with the risk of SpA development, in this study, and lead to significant changes in the innate and adaptive immune response profile, as well as the maintenance of the regulation of immunological mechanisms. Conclusion. The polymorphism rs5743708 for the TLR2 and the rs187084_rs5743836 TLR9 haplotypes appear to be involved in the development of clinical forms of SpA and can be a possible therapeutic target for the spondyloarthritis. |
| format | Article |
| id | doaj-art-1f9be33f6fdc4dcb9385c777da961a83 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-1f9be33f6fdc4dcb9385c777da961a832025-02-03T06:12:33ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/14920921492092Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic ArthritisCamila F. Oliveira-Toré0Amarilis G. Moraes1Gabriela F. Martinez2Janisleya S. F. Neves3Luciana C. Macedo4Marco A. Rocha-Loures5Marília G. Quirino6Hugo V. Alves7Ana M. Sell8Jeane E. L. Visentainer9Post Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilPost Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilDepartment of Medicine, Maringá State University, Parana, BrazilPost Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilPost Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilPost Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilPost Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilPost Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilPost Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilPost Graduation Program of Biosciences and Physiopathology, Department of Analysis Clinical and Biomedicine, Maringá State University, Parana, BrazilIntroduction. Ankylosing spondylitis (AS) and psoriatic arthritis (PsA) are classified as spondyloarthritis (SpA), a group of inflammatory rheumatic diseases with complex genetic etiology. Toll-like receptors (TLRs) have an important role in the mechanism of innate immunity and may influence inflammatory responses. Polymorphisms in TLR genes that lead to changes in these receptors or that interfere with the transcription rates of mRNA TLR may be involved in the chronic inflammatory immune response observed in SpA. Currently, there is a lack of studies associating genetic polymorphisms in TLRs and SpA. Objective. Therefore, this case-control study is aimed at analyzing the influence of the respective SNPs on TLR2 rs5743708, TLR6 rs5743810, and TLR9 rs5743836 and rs187084 in the immunopathogenesis of SpA. Methods. The polymorphisms genotyped by PCR-RFLP were TLR2 rs5743708, TLR6 rs5743810, and TLR9 rs5743836 and rs187084. The HLA-B∗27 was performed by PCR-SSP. Results. Logistic regression analysis showed a strong association between SNPs in TLR2 and TLR9 and susceptibility to SpA (OR=12.56; CI=6.5-25.9 and OR=1.62; CI=1.20-2.21, respectively). No association was observed among HLA-B∗27 and TLR polymorphisms (p=0.72), nor among BASDAI and TLR polymorphisms (p=0.85). Discussion. Our findings suggest that polymorphisms in TLR2 and TLR9 genes may contribute to the immunopathogenesis of the SpA. The rs187084, rs5743836, and rs5743708 polymorphisms were associated with the risk of SpA development, in this study, and lead to significant changes in the innate and adaptive immune response profile, as well as the maintenance of the regulation of immunological mechanisms. Conclusion. The polymorphism rs5743708 for the TLR2 and the rs187084_rs5743836 TLR9 haplotypes appear to be involved in the development of clinical forms of SpA and can be a possible therapeutic target for the spondyloarthritis.http://dx.doi.org/10.1155/2019/1492092 |
| spellingShingle | Camila F. Oliveira-Toré Amarilis G. Moraes Gabriela F. Martinez Janisleya S. F. Neves Luciana C. Macedo Marco A. Rocha-Loures Marília G. Quirino Hugo V. Alves Ana M. Sell Jeane E. L. Visentainer Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis Journal of Immunology Research |
| title | Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis |
| title_full | Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis |
| title_fullStr | Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis |
| title_full_unstemmed | Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis |
| title_short | Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis |
| title_sort | genetic polymorphisms of toll like receptors 2 and 9 as susceptibility factors for the development of ankylosing spondylitis and psoriatic arthritis |
| url | http://dx.doi.org/10.1155/2019/1492092 |
| work_keys_str_mv | AT camilafoliveiratore geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT amarilisgmoraes geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT gabrielafmartinez geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT janisleyasfneves geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT lucianacmacedo geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT marcoarochaloures geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT mariliagquirino geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT hugovalves geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT anamsell geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis AT jeaneelvisentainer geneticpolymorphismsoftolllikereceptors2and9assusceptibilityfactorsforthedevelopmentofankylosingspondylitisandpsoriaticarthritis |