Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice
Inflamm-aging indicates the chronic inflammatory state resulting from increased secretion of proinflammatory cytokines and mediators such as IL-6 in the elderly. Our principle objective was to identify cell types that were affected with aging concerning IL-6 secretion in the murine model. We compare...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/826987 |
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| _version_ | 1832558017776713728 |
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| author | Jihyun Park Takuya Miyakawa Aya Shiokawa Haruyo Nakajima-Adachi Masaru Tanokura Satoshi Hachimura |
| author_facet | Jihyun Park Takuya Miyakawa Aya Shiokawa Haruyo Nakajima-Adachi Masaru Tanokura Satoshi Hachimura |
| author_sort | Jihyun Park |
| collection | DOAJ |
| description | Inflamm-aging indicates the chronic inflammatory state resulting from increased secretion of proinflammatory cytokines and mediators such as IL-6 in the elderly. Our principle objective was to identify cell types that were affected with aging concerning IL-6 secretion in the murine model. We compared IL-6 production in spleen cells from both young and aged mice and isolated several types of cells from spleen and investigated IL-6 mRNA expression and protein production. IL-6 protein productions in cultured stromal cells from aged mice spleen were significantly high compared to young mice upon LPS stimulation. IL-6 mRNA expression level of freshly isolated stromal cells from aged mice was high compared to young mice. Furthermore, stromal cells of aged mice highly expressed IL-6 mRNA after LPS injection in vivo. These results suggest that stromal cells play a role in producing IL-6 in aged mice and imply that they contribute to the chronic inflammatory condition in the elderly. |
| format | Article |
| id | doaj-art-1f9bd2cb929348f9a5f0ce2dc703e3f5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1f9bd2cb929348f9a5f0ce2dc703e3f52025-02-03T01:33:23ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/826987826987Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young MiceJihyun Park0Takuya Miyakawa1Aya Shiokawa2Haruyo Nakajima-Adachi3Masaru Tanokura4Satoshi Hachimura5Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanResearch Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanResearch Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanResearch Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanInflamm-aging indicates the chronic inflammatory state resulting from increased secretion of proinflammatory cytokines and mediators such as IL-6 in the elderly. Our principle objective was to identify cell types that were affected with aging concerning IL-6 secretion in the murine model. We compared IL-6 production in spleen cells from both young and aged mice and isolated several types of cells from spleen and investigated IL-6 mRNA expression and protein production. IL-6 protein productions in cultured stromal cells from aged mice spleen were significantly high compared to young mice upon LPS stimulation. IL-6 mRNA expression level of freshly isolated stromal cells from aged mice was high compared to young mice. Furthermore, stromal cells of aged mice highly expressed IL-6 mRNA after LPS injection in vivo. These results suggest that stromal cells play a role in producing IL-6 in aged mice and imply that they contribute to the chronic inflammatory condition in the elderly.http://dx.doi.org/10.1155/2014/826987 |
| spellingShingle | Jihyun Park Takuya Miyakawa Aya Shiokawa Haruyo Nakajima-Adachi Masaru Tanokura Satoshi Hachimura Splenic Stromal Cells from Aged Mice Produce Higher Levels of IL-6 Compared to Young Mice Mediators of Inflammation |
| title | Splenic Stromal Cells from Aged Mice Produce Higher Levels of
IL-6 Compared to Young Mice |
| title_full | Splenic Stromal Cells from Aged Mice Produce Higher Levels of
IL-6 Compared to Young Mice |
| title_fullStr | Splenic Stromal Cells from Aged Mice Produce Higher Levels of
IL-6 Compared to Young Mice |
| title_full_unstemmed | Splenic Stromal Cells from Aged Mice Produce Higher Levels of
IL-6 Compared to Young Mice |
| title_short | Splenic Stromal Cells from Aged Mice Produce Higher Levels of
IL-6 Compared to Young Mice |
| title_sort | splenic stromal cells from aged mice produce higher levels of il 6 compared to young mice |
| url | http://dx.doi.org/10.1155/2014/826987 |
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