Development of a Magnetic LED-based Sterilisator for Inactivation of Contaminant Bacteria
Nosocomial infections are caused by viruses, bacteria, and fungi that patients acquire while receiving treatment in a healthcare facility. These infections often result from cross-contamination, either through the hands of healthcare workers or contaminated medical equipment. Staphylococcus aureus i...
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Erbil Polytechnic University
2024-11-01
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| Series: | Polytechnic |
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| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol14/iss2/6 |
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| author | Yonatan . Suryani Dyah Astuti Khusnul Ain Deny Arifianto Ahmad Khalil Yaqubi Perwira Annissa Dyah Permatasari |
| author_facet | Yonatan . Suryani Dyah Astuti Khusnul Ain Deny Arifianto Ahmad Khalil Yaqubi Perwira Annissa Dyah Permatasari |
| author_sort | Yonatan . |
| collection | DOAJ |
| description | Nosocomial infections are caused by viruses, bacteria, and fungi that patients acquire while receiving treatment in a
healthcare facility. These infections often result from cross-contamination, either through the hands of healthcare
workers or contaminated medical equipment. Staphylococcus aureus is a well-known pathogen responsible for many
infections, from mild skin conditions to severe sepsis or multiorgan failure. Sterilization is a process designed to
eliminate or destroy all forms of microorganisms, including bacteria, fungi, and viruses, on objects or in environments,
preventing them from reproducing or causing infections. Static Magnetic Fields (SMF) and Photodynamic Inactivation
(PDI), two more recent technologies, provide alternatives to conventional treatments like autoclaving, which have
drawbacks. While SMF damages cellular structures, PDI utilizes blue light to create ROS that harm bacteria. PDI and
SMF together improve bacterial inactivation, especially S. aureus. This study aims to evaluate combining a static magnetic field with photodynamic inactivation to the inactivation of S. aureus (a Gram-positive bacterium). The research
utilized high-power blue LEDs and static magnetic fields generated by neodymium magnets. The highest reduction in
bacterial count, 81.92% ± 7.92%, was observed with the combined treatment of static magnetic fields and LED illumination at the F8 microplate location, with an exposure time of 40 min. The LED energy density was 11.72 J/cm2
, and the
static magnetic field strength was 25.61 mT. Conversely, the lowest reduction, 52.41% ± 7.64%, occurred at the exact F8
microplate location with a 10-min exposure, an LED energy density of 2.93 J/cm2
, and a static magnetic field strength of
25.61 mT |
| format | Article |
| id | doaj-art-1f7f96ab8ea14246880e6b1f07a5f284 |
| institution | Kabale University |
| issn | 2707-7799 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Erbil Polytechnic University |
| record_format | Article |
| series | Polytechnic |
| spelling | doaj-art-1f7f96ab8ea14246880e6b1f07a5f2842025-01-28T17:50:39ZengErbil Polytechnic UniversityPolytechnic2707-77992024-11-011425967https://doi.org/10.59341/2707-7799.1839Development of a Magnetic LED-based Sterilisator for Inactivation of Contaminant BacteriaYonatan .0Suryani Dyah Astuti1Khusnul Ain2Deny Arifianto3Ahmad Khalil Yaqubi4Perwira Annissa Dyah Permatasari5Magister Program of Biomedical Engineering, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, IndonesiaDepartment of Physics, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, IndonesiaDepartment of Engineering, Faculty of Vocational, Univeristas Airlangga, Surabaya, IndonesiaDepartment of Engineering, Faculty of Vocational, Univeristas Airlangga, Surabaya, IndonesiaDoctoral Program of Mathematics and Natural Science, Faculty of Science and Technology, Universitas Airlangga, 60115, Surabaya, IndonesiaDepartment of Mathematics, Faculty of Science and Technology Airlangga University, Surabaya, 60115, IndonesiaNosocomial infections are caused by viruses, bacteria, and fungi that patients acquire while receiving treatment in a healthcare facility. These infections often result from cross-contamination, either through the hands of healthcare workers or contaminated medical equipment. Staphylococcus aureus is a well-known pathogen responsible for many infections, from mild skin conditions to severe sepsis or multiorgan failure. Sterilization is a process designed to eliminate or destroy all forms of microorganisms, including bacteria, fungi, and viruses, on objects or in environments, preventing them from reproducing or causing infections. Static Magnetic Fields (SMF) and Photodynamic Inactivation (PDI), two more recent technologies, provide alternatives to conventional treatments like autoclaving, which have drawbacks. While SMF damages cellular structures, PDI utilizes blue light to create ROS that harm bacteria. PDI and SMF together improve bacterial inactivation, especially S. aureus. This study aims to evaluate combining a static magnetic field with photodynamic inactivation to the inactivation of S. aureus (a Gram-positive bacterium). The research utilized high-power blue LEDs and static magnetic fields generated by neodymium magnets. The highest reduction in bacterial count, 81.92% ± 7.92%, was observed with the combined treatment of static magnetic fields and LED illumination at the F8 microplate location, with an exposure time of 40 min. The LED energy density was 11.72 J/cm2 , and the static magnetic field strength was 25.61 mT. Conversely, the lowest reduction, 52.41% ± 7.64%, occurred at the exact F8 microplate location with a 10-min exposure, an LED energy density of 2.93 J/cm2 , and a static magnetic field strength of 25.61 mThttps://polytechnic-journal.epu.edu.iq/home/vol14/iss2/6nosocomial infections,photodynamic inactivation (pdi),static magnetic field (smf),high power led,staphylococcus aureus |
| spellingShingle | Yonatan . Suryani Dyah Astuti Khusnul Ain Deny Arifianto Ahmad Khalil Yaqubi Perwira Annissa Dyah Permatasari Development of a Magnetic LED-based Sterilisator for Inactivation of Contaminant Bacteria Polytechnic nosocomial infections, photodynamic inactivation (pdi), static magnetic field (smf), high power led, staphylococcus aureus |
| title | Development of a Magnetic LED-based Sterilisator for Inactivation of Contaminant Bacteria |
| title_full | Development of a Magnetic LED-based Sterilisator for Inactivation of Contaminant Bacteria |
| title_fullStr | Development of a Magnetic LED-based Sterilisator for Inactivation of Contaminant Bacteria |
| title_full_unstemmed | Development of a Magnetic LED-based Sterilisator for Inactivation of Contaminant Bacteria |
| title_short | Development of a Magnetic LED-based Sterilisator for Inactivation of Contaminant Bacteria |
| title_sort | development of a magnetic led based sterilisator for inactivation of contaminant bacteria |
| topic | nosocomial infections, photodynamic inactivation (pdi), static magnetic field (smf), high power led, staphylococcus aureus |
| url | https://polytechnic-journal.epu.edu.iq/home/vol14/iss2/6 |
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