Distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periods
<b>Aim: </b>This study aimed to determine the distribution and heterogeneity of mast cells located in different parts of the genital tract of female quails (Coturnix coturnix japonica) during the prepubertal and postpubertal periods using different fixation solutions and staining methods...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2022-12-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1405 |
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| author | Nariste Kadyralieva Hasan Huseyin Donmez |
| author_facet | Nariste Kadyralieva Hasan Huseyin Donmez |
| author_sort | Nariste Kadyralieva |
| collection | DOAJ |
| description | <b>Aim: </b>This study aimed to determine the distribution and heterogeneity of
mast cells located in different parts of the genital tract of female quails (Coturnix
coturnix japonica) during the prepubertal and postpubertal periods using
different fixation solutions and staining methods.<p>
<b>Materials and Methods: </b>For this purpose, tissue samples were taken from
the genital tract (infundibulum, magnum, isthmus, uterus, vagina) of healthy
female quails, including 10 prepubertal and 10 postpubertal animals. The
tissue samples of both groups were fixed in either 10% neutral formaldehyde
or isotonic formaldehyde acetic acid solution. The tissue sections were stained
with the toluidine blue and combined alcian blue/safranin O staining methods.<p>
<b>Results: </b>The light microscopic examination of the tissue sections treated with
both fixatives demonstrated that mast cells were round to oval or spindle-like
in form and localized especially around blood vessels in the tunica mucosa,
tunica muscularis and tunica serosa layers. Mast cells were found to be
distributed primarily in the uterus during the prepubertal and postpubertal
periods; followed in descending order by the isthmus, magnum, vagina and
infundibulum. It was determined that mast cell counts significantly increased
in the infundibulum in the postpubertal period (p<0.05) compared to the
prepubertal period. Fixation in IFAA was associated with a more prominent
staining and higher count of mast cells in all tissues, compared to fixation in
formaldehyde.<p>
<b>Conclusion: </b>The number of mast cells in the infundibulum was determined to
have increased in the postpubertal period, and this increase was considered to
may affect egg formation and egg-laying. |
| format | Article |
| id | doaj-art-1f757b947184470cbda800facc0415c6 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-1f757b947184470cbda800facc0415c62025-02-03T11:25:37ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532022-12-013842062131405Distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periodsNariste Kadyralieva0Hasan Huseyin Donmez1Kyrgyzstan Turkey Manas University, Veterinary Faculty, Department of Histology and Embryology, Bishkek, KyrgyzstanSelcuk University, Veterinary Faculty, Department of Histology and Embryology, Konya, Turkey<b>Aim: </b>This study aimed to determine the distribution and heterogeneity of mast cells located in different parts of the genital tract of female quails (Coturnix coturnix japonica) during the prepubertal and postpubertal periods using different fixation solutions and staining methods.<p> <b>Materials and Methods: </b>For this purpose, tissue samples were taken from the genital tract (infundibulum, magnum, isthmus, uterus, vagina) of healthy female quails, including 10 prepubertal and 10 postpubertal animals. The tissue samples of both groups were fixed in either 10% neutral formaldehyde or isotonic formaldehyde acetic acid solution. The tissue sections were stained with the toluidine blue and combined alcian blue/safranin O staining methods.<p> <b>Results: </b>The light microscopic examination of the tissue sections treated with both fixatives demonstrated that mast cells were round to oval or spindle-like in form and localized especially around blood vessels in the tunica mucosa, tunica muscularis and tunica serosa layers. Mast cells were found to be distributed primarily in the uterus during the prepubertal and postpubertal periods; followed in descending order by the isthmus, magnum, vagina and infundibulum. It was determined that mast cell counts significantly increased in the infundibulum in the postpubertal period (p<0.05) compared to the prepubertal period. Fixation in IFAA was associated with a more prominent staining and higher count of mast cells in all tissues, compared to fixation in formaldehyde.<p> <b>Conclusion: </b>The number of mast cells in the infundibulum was determined to have increased in the postpubertal period, and this increase was considered to may affect egg formation and egg-laying.http://eurasianjvetsci.org/pdf.php3?id=1405genital canalmast cell distributionquail |
| spellingShingle | Nariste Kadyralieva Hasan Huseyin Donmez Distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periods Eurasian Journal of Veterinary Sciences genital canal mast cell distribution quail |
| title | Distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periods |
| title_full | Distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periods |
| title_fullStr | Distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periods |
| title_full_unstemmed | Distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periods |
| title_short | Distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periods |
| title_sort | distribution and heterogeneity of mast cells in different regions of the genital canal in female quails during the prepubertal and postpubertal periods |
| topic | genital canal mast cell distribution quail |
| url | http://eurasianjvetsci.org/pdf.php3?id=1405 |
| work_keys_str_mv | AT naristekadyralieva distributionandheterogeneityofmastcellsindifferentregionsofthegenitalcanalinfemalequailsduringtheprepubertalandpostpubertalperiods AT hasanhuseyindonmez distributionandheterogeneityofmastcellsindifferentregionsofthegenitalcanalinfemalequailsduringtheprepubertalandpostpubertalperiods |