Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairing
ABSTRACT Bacteriophages (phages) are bacterial-specific viruses that can be used alone or with antibiotics to reduce bacterial load. Most phages are unsuitable for therapy because they are “temperate” and can integrate into the host genome, forming a lysogen that is protected from subsequent phage i...
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American Society for Microbiology
2025-02-01
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| Online Access: | https://journals.asm.org/doi/10.1128/mbio.02559-24 |
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| author | Rabia Fatima Alexander P. Hynes |
| author_facet | Rabia Fatima Alexander P. Hynes |
| author_sort | Rabia Fatima |
| collection | DOAJ |
| description | ABSTRACT Bacteriophages (phages) are bacterial-specific viruses that can be used alone or with antibiotics to reduce bacterial load. Most phages are unsuitable for therapy because they are “temperate” and can integrate into the host genome, forming a lysogen that is protected from subsequent phage infections. However, integrated phages can be awakened by stressors such as antibiotics. Supported by this interaction, here we explore the potential use of combined temperate phage and antibiotic against the multi-drug-resistant pathogen, Pseudomonas aeruginosa. In all, thirty-nine temperate phages were isolated from clinical strains, and a subset was screened for synergy with six antibiotics (ciprofloxacin, levofloxacin, meropenem, piperacillin, tobramycin, and polymyxin B), using checkerboard assays. Interestingly, our screen identified phages that can synergize with each antibiotic, despite their widely differing targets; however, these are highly phage-antibiotic and phage-host pairing specific. Screening across multiple clinical strains reveals that temperate phages can reduce the antibiotic minimum inhibitory concentration up to 32-fold, even in a resistant isolate, functionally re-sensitizing the bacterium to the antibiotic. Meropenem and tobramycin did not reduce the frequency of lysogens, suggesting a mechanism of action independent of the temperate nature of the phages. By contrast, ciprofloxacin and piperacillin were able to reduce the frequency of lysogeny, the former by inducing phages—as previously reported in E. coli. Curiously, synergy with piperacillin reduced lysogen survivors, but not by inducing the phages, suggesting an alternative mechanism for biasing the phage lysis-lysogeny equilibrium. Overall, our findings indicate that temperate phages can act as adjuvants in clinically relevant pathogens, even in the presence of antibiotic resistance, thereby drastically expanding their therapeutic potential.IMPORTANCEThe recent discovery that otherwise therapeutically unusable temperate phages can potentiate the activity of antibiotics, resulting in a potent synergy, has only been tested in E. coli, and with a single model phage. Here, working with clinical isolates of Pseudomonas and phages from these isolates, we highlight the broad applicability of this synergy—across a variety of mechanisms but also highlight the limitations of predicting the phage, host, and antibiotic combinations that will synergize. |
| format | Article |
| id | doaj-art-1f611548969b488bbce7af8c5f9bd34a |
| institution | Kabale University |
| issn | 2150-7511 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj-art-1f611548969b488bbce7af8c5f9bd34a2025-02-05T14:00:48ZengAmerican Society for MicrobiologymBio2150-75112025-02-0116210.1128/mbio.02559-24Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairingRabia Fatima0Alexander P. Hynes1Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, CanadaDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, CanadaABSTRACT Bacteriophages (phages) are bacterial-specific viruses that can be used alone or with antibiotics to reduce bacterial load. Most phages are unsuitable for therapy because they are “temperate” and can integrate into the host genome, forming a lysogen that is protected from subsequent phage infections. However, integrated phages can be awakened by stressors such as antibiotics. Supported by this interaction, here we explore the potential use of combined temperate phage and antibiotic against the multi-drug-resistant pathogen, Pseudomonas aeruginosa. In all, thirty-nine temperate phages were isolated from clinical strains, and a subset was screened for synergy with six antibiotics (ciprofloxacin, levofloxacin, meropenem, piperacillin, tobramycin, and polymyxin B), using checkerboard assays. Interestingly, our screen identified phages that can synergize with each antibiotic, despite their widely differing targets; however, these are highly phage-antibiotic and phage-host pairing specific. Screening across multiple clinical strains reveals that temperate phages can reduce the antibiotic minimum inhibitory concentration up to 32-fold, even in a resistant isolate, functionally re-sensitizing the bacterium to the antibiotic. Meropenem and tobramycin did not reduce the frequency of lysogens, suggesting a mechanism of action independent of the temperate nature of the phages. By contrast, ciprofloxacin and piperacillin were able to reduce the frequency of lysogeny, the former by inducing phages—as previously reported in E. coli. Curiously, synergy with piperacillin reduced lysogen survivors, but not by inducing the phages, suggesting an alternative mechanism for biasing the phage lysis-lysogeny equilibrium. Overall, our findings indicate that temperate phages can act as adjuvants in clinically relevant pathogens, even in the presence of antibiotic resistance, thereby drastically expanding their therapeutic potential.IMPORTANCEThe recent discovery that otherwise therapeutically unusable temperate phages can potentiate the activity of antibiotics, resulting in a potent synergy, has only been tested in E. coli, and with a single model phage. Here, working with clinical isolates of Pseudomonas and phages from these isolates, we highlight the broad applicability of this synergy—across a variety of mechanisms but also highlight the limitations of predicting the phage, host, and antibiotic combinations that will synergize.https://journals.asm.org/doi/10.1128/mbio.02559-24bacteriophagesantibioticstemperate phagesphage-antibiotic synergyPseudomonas aeruginosaclinical isolates |
| spellingShingle | Rabia Fatima Alexander P. Hynes Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairing mBio bacteriophages antibiotics temperate phages phage-antibiotic synergy Pseudomonas aeruginosa clinical isolates |
| title | Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairing |
| title_full | Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairing |
| title_fullStr | Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairing |
| title_full_unstemmed | Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairing |
| title_short | Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairing |
| title_sort | temperate phage antibiotic synergy is widespread extending to pseudomonas but varies by phage host strain and antibiotic pairing |
| topic | bacteriophages antibiotics temperate phages phage-antibiotic synergy Pseudomonas aeruginosa clinical isolates |
| url | https://journals.asm.org/doi/10.1128/mbio.02559-24 |
| work_keys_str_mv | AT rabiafatima temperatephageantibioticsynergyiswidespreadextendingtopseudomonasbutvariesbyphagehoststrainandantibioticpairing AT alexanderphynes temperatephageantibioticsynergyiswidespreadextendingtopseudomonasbutvariesbyphagehoststrainandantibioticpairing |