Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection
Avian reovirus (ARV), a double-stranded RNA virus, frequently induces immunosuppression in poultry, leading to symptoms such as irregular bleeding and spleen necrosis in infected ducks. Since 2017, the morbidity and mortality rates associated with ARV infection in poultry have been on the rise, prog...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1539903/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832539875674423296 |
|---|---|
| author | Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Jinkang Li Mingshu Wang Mingshu Wang Mingshu Wang Mingshu Wang Renyong Jia Renyong Jia Renyong Jia Renyong Jia Shun Chen Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Mafeng Liu Dekang Zhu Dekang Zhu Dekang Zhu Dekang Zhu Xinxin Zhao Xinxin Zhao Xinxin Zhao Xinxin Zhao Ying Wu Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Qiao Yang Juan Huang Juan Huang Juan Huang Juan Huang Xumin Ou Xumin Ou Xumin Ou Xumin Ou Di Sun Di Sun Di Sun Di Sun Bin Tian Bin Tian Bin Tian Bin Tian Yu He Yu He Yu He Yu He Zhen Wu Zhen Wu Zhen Wu Zhen Wu Anchun Cheng Anchun Cheng Anchun Cheng Anchun Cheng |
| author_facet | Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Jinkang Li Mingshu Wang Mingshu Wang Mingshu Wang Mingshu Wang Renyong Jia Renyong Jia Renyong Jia Renyong Jia Shun Chen Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Mafeng Liu Dekang Zhu Dekang Zhu Dekang Zhu Dekang Zhu Xinxin Zhao Xinxin Zhao Xinxin Zhao Xinxin Zhao Ying Wu Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Qiao Yang Juan Huang Juan Huang Juan Huang Juan Huang Xumin Ou Xumin Ou Xumin Ou Xumin Ou Di Sun Di Sun Di Sun Di Sun Bin Tian Bin Tian Bin Tian Bin Tian Yu He Yu He Yu He Yu He Zhen Wu Zhen Wu Zhen Wu Zhen Wu Anchun Cheng Anchun Cheng Anchun Cheng Anchun Cheng |
| author_sort | Shaqiu Zhang |
| collection | DOAJ |
| description | Avian reovirus (ARV), a double-stranded RNA virus, frequently induces immunosuppression in poultry, leading to symptoms such as irregular bleeding and spleen necrosis in infected ducks. Since 2017, the morbidity and mortality rates associated with ARV infection in poultry have been on the rise, progressively emerging as a significant viral disease impacting the duck farming industry in China. In our study, we collected duck embryo fibroblasts 18 h post-infection with ARV and conducted transcriptome sequencing analysis. The analysis revealed that 3,818 mRNA expressions were up-regulated, 4,573 mRNA expressions were down-regulated, 472 long noncoding RNAs (LncRNAs) were up-regulated, and 345 lncRNAs were down-regulated. We employed qRT-PCR to validate the sequencing results, confirming their accuracy. The transcriptome data indicated significant upregulation of the PARP9, TLR7, TRIM33, and ATG5 genes, suggesting their potential involvement in ARV infection. Notably, our study identified a novel functional lncRNA, MSTRG.9284.1 (It was named linc000889 in the present study), which inhibits the replication of ARV at the transcriptional, translational levels and viral titer. Overall, this study has identified numerous ARV-induced differentially expressed mRNAs and lncRNAs, including the functional lncRNA linc000889 that inhibits ARV replication. This discovery provides new insights into the mechanisms of ARV infection and may contribute to the development of new prevention and treatment strategies. |
| format | Article |
| id | doaj-art-1f27e8acb0234d589e5ba773946e6654 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-1f27e8acb0234d589e5ba773946e66542025-02-05T07:32:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15399031539903Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infectionShaqiu Zhang0Shaqiu Zhang1Shaqiu Zhang2Shaqiu Zhang3Jinkang Li4Mingshu Wang5Mingshu Wang6Mingshu Wang7Mingshu Wang8Renyong Jia9Renyong Jia10Renyong Jia11Renyong Jia12Shun Chen13Shun Chen14Shun Chen15Shun Chen16Mafeng Liu17Mafeng Liu18Mafeng Liu19Mafeng Liu20Dekang Zhu21Dekang Zhu22Dekang Zhu23Dekang Zhu24Xinxin Zhao25Xinxin Zhao26Xinxin Zhao27Xinxin Zhao28Ying Wu29Ying Wu30Ying Wu31Ying Wu32Qiao Yang33Qiao Yang34Qiao Yang35Qiao Yang36Juan Huang37Juan Huang38Juan Huang39Juan Huang40Xumin Ou41Xumin Ou42Xumin Ou43Xumin Ou44Di Sun45Di Sun46Di Sun47Di Sun48Bin Tian49Bin Tian50Bin Tian51Bin Tian52Yu He53Yu He54Yu He55Yu He56Zhen Wu57Zhen Wu58Zhen Wu59Zhen Wu60Anchun Cheng61Anchun Cheng62Anchun Cheng63Anchun Cheng64Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the P.R. China, Chengdu, ChinaAvian reovirus (ARV), a double-stranded RNA virus, frequently induces immunosuppression in poultry, leading to symptoms such as irregular bleeding and spleen necrosis in infected ducks. Since 2017, the morbidity and mortality rates associated with ARV infection in poultry have been on the rise, progressively emerging as a significant viral disease impacting the duck farming industry in China. In our study, we collected duck embryo fibroblasts 18 h post-infection with ARV and conducted transcriptome sequencing analysis. The analysis revealed that 3,818 mRNA expressions were up-regulated, 4,573 mRNA expressions were down-regulated, 472 long noncoding RNAs (LncRNAs) were up-regulated, and 345 lncRNAs were down-regulated. We employed qRT-PCR to validate the sequencing results, confirming their accuracy. The transcriptome data indicated significant upregulation of the PARP9, TLR7, TRIM33, and ATG5 genes, suggesting their potential involvement in ARV infection. Notably, our study identified a novel functional lncRNA, MSTRG.9284.1 (It was named linc000889 in the present study), which inhibits the replication of ARV at the transcriptional, translational levels and viral titer. Overall, this study has identified numerous ARV-induced differentially expressed mRNAs and lncRNAs, including the functional lncRNA linc000889 that inhibits ARV replication. This discovery provides new insights into the mechanisms of ARV infection and may contribute to the development of new prevention and treatment strategies.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1539903/fullavian reovirustranscriptome sequencinglong noncoding RNAlinc000889avian reovirus replication |
| spellingShingle | Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Jinkang Li Mingshu Wang Mingshu Wang Mingshu Wang Mingshu Wang Renyong Jia Renyong Jia Renyong Jia Renyong Jia Shun Chen Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Mafeng Liu Dekang Zhu Dekang Zhu Dekang Zhu Dekang Zhu Xinxin Zhao Xinxin Zhao Xinxin Zhao Xinxin Zhao Ying Wu Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Qiao Yang Juan Huang Juan Huang Juan Huang Juan Huang Xumin Ou Xumin Ou Xumin Ou Xumin Ou Di Sun Di Sun Di Sun Di Sun Bin Tian Bin Tian Bin Tian Bin Tian Yu He Yu He Yu He Yu He Zhen Wu Zhen Wu Zhen Wu Zhen Wu Anchun Cheng Anchun Cheng Anchun Cheng Anchun Cheng Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection Frontiers in Microbiology avian reovirus transcriptome sequencing long noncoding RNA linc000889 avian reovirus replication |
| title | Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection |
| title_full | Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection |
| title_fullStr | Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection |
| title_full_unstemmed | Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection |
| title_short | Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection |
| title_sort | comprehensive analysis of lncrnas and mrnas revealed potential participants in the process of avian reovirus infection |
| topic | avian reovirus transcriptome sequencing long noncoding RNA linc000889 avian reovirus replication |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1539903/full |
| work_keys_str_mv | AT shaqiuzhang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT shaqiuzhang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT shaqiuzhang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT shaqiuzhang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT jinkangli comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT mingshuwang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT mingshuwang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT mingshuwang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT mingshuwang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT renyongjia comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT renyongjia comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT renyongjia comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT renyongjia comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT shunchen comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT shunchen comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT shunchen comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT shunchen comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT mafengliu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT mafengliu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT mafengliu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT mafengliu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT dekangzhu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT dekangzhu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT dekangzhu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT dekangzhu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT xinxinzhao comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT xinxinzhao comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT xinxinzhao comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT xinxinzhao comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT yingwu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT yingwu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT yingwu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT yingwu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT qiaoyang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT qiaoyang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT qiaoyang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT qiaoyang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT juanhuang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT juanhuang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT juanhuang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT juanhuang comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT xuminou comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT xuminou comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT xuminou comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT xuminou comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT disun comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT disun comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT disun comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT disun comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT bintian comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT bintian comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT bintian comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT bintian comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT yuhe comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT yuhe comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT yuhe comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT yuhe comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT zhenwu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT zhenwu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT zhenwu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT zhenwu comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT anchuncheng comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT anchuncheng comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT anchuncheng comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection AT anchuncheng comprehensiveanalysisoflncrnasandmrnasrevealedpotentialparticipantsintheprocessofavianreovirusinfection |