SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway
Abstract Objective Osteoarthritis (OA) is the most common form of joint disease. Currently, OA treatment is limited to controlling symptoms. Our previous study showed that stromal cell-derived factor 1 (SDF-1) delayed the progression of OA to a certain extent. The aim of this study was to explore th...
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BMC
2025-03-01
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| Series: | Arthritis Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13075-025-03509-8 |
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| author | Yanping Zhao Dan Lin Xiaoying Zhu Jingyao Yan Yan Liang Yanli Wang Tianqi Dai Zhiyi Zhang Shuya Wang |
| author_facet | Yanping Zhao Dan Lin Xiaoying Zhu Jingyao Yan Yan Liang Yanli Wang Tianqi Dai Zhiyi Zhang Shuya Wang |
| author_sort | Yanping Zhao |
| collection | DOAJ |
| description | Abstract Objective Osteoarthritis (OA) is the most common form of joint disease. Currently, OA treatment is limited to controlling symptoms. Our previous study showed that stromal cell-derived factor 1 (SDF-1) delayed the progression of OA to a certain extent. The aim of this study was to explore the specific mechanism of SDF-1 in OA. Materials and methods OA chondrocytes and a collagen-induced osteoarthritis (CIOA) mouse model were used as in vitro and in vivo models, respectively. SDF-1 was used to treat OA in vitro and in vivo. To explore the mechanism of SDF-1 in OA treatment, we pretreated chondrocytes with a Sirt 3 inhibitor and assessed mitochondrial function and then analysed related indicators of cartilage anabolic and cartilage metabolism. Results SOD2 and PGC-1α levels were significantly lower in OA chondrocytes and the cartilage of CIOA model mice than in normal chondrocytes, and mitochondrial dysfunction occurred in OA. After treating OA chondrocytes and CIOA model mice with exogenous SDF-1, mitochondrial dysfunction and abnormal biomarkers of OA normalized. The pretreatment of OA chondrocytes with a Sirt 3 inhibitor or mitochondrial function inhibitor before SDF-1 exposure reversed these changes. Conclusions SDF-1 can alleviate OA by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway, and therefore, SDF-1 may be a good candidate as a new treatment for OA. |
| format | Article |
| id | doaj-art-1f22c17fca3e481d91b4ddb1a2977bb5 |
| institution | OA Journals |
| issn | 1478-6362 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Arthritis Research & Therapy |
| spelling | doaj-art-1f22c17fca3e481d91b4ddb1a2977bb52025-08-20T01:57:52ZengBMCArthritis Research & Therapy1478-63622025-03-012711910.1186/s13075-025-03509-8SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathwayYanping Zhao0Dan Lin1Xiaoying Zhu2Jingyao Yan3Yan Liang4Yanli Wang5Tianqi Dai6Zhiyi Zhang7Shuya Wang8Department of Rheumatology, The Affiliated hospital of Harbin Medical UniversityDepartment of Rheumatology, The Affiliated hospital of Harbin Medical UniversityDepartment of Rheumatology, The Affiliated hospital of Harbin Medical UniversityDepartment of Rheumatology, The Affiliated hospital of Harbin Medical UniversityDepartment of Rheumatology, The Affiliated hospital of Harbin Medical UniversityDepartment of Rheumatology, The Affiliated hospital of Harbin Medical UniversityDepartment of Rheumatology, The Affiliated hospital of Harbin Medical UniversityDepartment of Rheumatology, The Affiliated hospital of Harbin Medical UniversityDepartment of Rheumatology, The Affiliated hospital of Harbin Medical UniversityAbstract Objective Osteoarthritis (OA) is the most common form of joint disease. Currently, OA treatment is limited to controlling symptoms. Our previous study showed that stromal cell-derived factor 1 (SDF-1) delayed the progression of OA to a certain extent. The aim of this study was to explore the specific mechanism of SDF-1 in OA. Materials and methods OA chondrocytes and a collagen-induced osteoarthritis (CIOA) mouse model were used as in vitro and in vivo models, respectively. SDF-1 was used to treat OA in vitro and in vivo. To explore the mechanism of SDF-1 in OA treatment, we pretreated chondrocytes with a Sirt 3 inhibitor and assessed mitochondrial function and then analysed related indicators of cartilage anabolic and cartilage metabolism. Results SOD2 and PGC-1α levels were significantly lower in OA chondrocytes and the cartilage of CIOA model mice than in normal chondrocytes, and mitochondrial dysfunction occurred in OA. After treating OA chondrocytes and CIOA model mice with exogenous SDF-1, mitochondrial dysfunction and abnormal biomarkers of OA normalized. The pretreatment of OA chondrocytes with a Sirt 3 inhibitor or mitochondrial function inhibitor before SDF-1 exposure reversed these changes. Conclusions SDF-1 can alleviate OA by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway, and therefore, SDF-1 may be a good candidate as a new treatment for OA.https://doi.org/10.1186/s13075-025-03509-8OsteoarthritisStromal cell-derived factor 1Mitochondrial dysfunctionChondrocyteSirt3 |
| spellingShingle | Yanping Zhao Dan Lin Xiaoying Zhu Jingyao Yan Yan Liang Yanli Wang Tianqi Dai Zhiyi Zhang Shuya Wang SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway Arthritis Research & Therapy Osteoarthritis Stromal cell-derived factor 1 Mitochondrial dysfunction Chondrocyte Sirt3 |
| title | SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway |
| title_full | SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway |
| title_fullStr | SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway |
| title_full_unstemmed | SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway |
| title_short | SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway |
| title_sort | sdf 1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the sirt3 pgc 1α signalling pathway |
| topic | Osteoarthritis Stromal cell-derived factor 1 Mitochondrial dysfunction Chondrocyte Sirt3 |
| url | https://doi.org/10.1186/s13075-025-03509-8 |
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