Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures
ATP-binding cassette subfamily C (ABCC) genes code for phase III metabolism proteins that translocate xenobiotic (e.g., particulate matter 2.5 (PM2.5)) and drug metabolites outside the cells. IL-6 secretion is related with the activation of the ABCC transporters. This study assesses ABCC1–4 gene exp...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/6827194 |
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| author | Jarline Encarnación-Medina Rosa I. Rodríguez-Cotto Joseph Bloom-Oquendo Mario G. Ortiz-Martínez Jorge Duconge Braulio Jiménez-Vélez |
| author_facet | Jarline Encarnación-Medina Rosa I. Rodríguez-Cotto Joseph Bloom-Oquendo Mario G. Ortiz-Martínez Jorge Duconge Braulio Jiménez-Vélez |
| author_sort | Jarline Encarnación-Medina |
| collection | DOAJ |
| description | ATP-binding cassette subfamily C (ABCC) genes code for phase III metabolism proteins that translocate xenobiotic (e.g., particulate matter 2.5 (PM2.5)) and drug metabolites outside the cells. IL-6 secretion is related with the activation of the ABCC transporters. This study assesses ABCC1–4 gene expression changes and proinflammatory cytokine (IL-6, IL-8) release in human bronchial epithelial cells (BEAS-2B) exposed to PM2.5 organic extract, budesonide (BUD, used to control inflammation in asthmatic patients), and a cotreatment (Co-T: PM2.5 and BUD). A real-time PCR assay shows that ABCC1 was upregulated in BEAS-2B exposed after 6 and 7 hr to PM2.5 extract or BUD but downregulated after 6 hr of the Co-T. ABCC3 was downregulated after 6 hr of BUD and upregulated after 6 hr of the Co-T exposures. ABCC4 was upregulated after 5 hr of PM2.5 extract, BUD, and the Co-T exposures. The cytokine assay revealed an increase in IL-6 release by BEAS-2B exposed after 5 hr to PM2.5 extract, BUD, and the Co-T. At 7 hr, the Co-T decreases IL-6 release and IL-8 at 6 hr. In conclusion, the cotreatment showed an opposite effect on exposed BEAS-2B as compared with BUD. The results suggest an interference of the BUD therapeutic potential by PM2.5. |
| format | Article |
| id | doaj-art-1f07ed383a4b4f919fb9387596a05744 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1f07ed383a4b4f919fb9387596a057442025-02-03T01:11:12ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/68271946827194Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated ExposuresJarline Encarnación-Medina0Rosa I. Rodríguez-Cotto1Joseph Bloom-Oquendo2Mario G. Ortiz-Martínez3Jorge Duconge4Braulio Jiménez-Vélez5School of Pharmacy, University of Puerto Rico, Medical Science Campus, San Juan, PR, USACenter for Environmental and Toxicological Research, San Juan, PR, USASchool of Pharmacy, University of Puerto Rico, Medical Science Campus, San Juan, PR, USACenter for Environmental and Toxicological Research, San Juan, PR, USASchool of Pharmacy, University of Puerto Rico, Medical Science Campus, San Juan, PR, USACenter for Environmental and Toxicological Research, San Juan, PR, USAATP-binding cassette subfamily C (ABCC) genes code for phase III metabolism proteins that translocate xenobiotic (e.g., particulate matter 2.5 (PM2.5)) and drug metabolites outside the cells. IL-6 secretion is related with the activation of the ABCC transporters. This study assesses ABCC1–4 gene expression changes and proinflammatory cytokine (IL-6, IL-8) release in human bronchial epithelial cells (BEAS-2B) exposed to PM2.5 organic extract, budesonide (BUD, used to control inflammation in asthmatic patients), and a cotreatment (Co-T: PM2.5 and BUD). A real-time PCR assay shows that ABCC1 was upregulated in BEAS-2B exposed after 6 and 7 hr to PM2.5 extract or BUD but downregulated after 6 hr of the Co-T. ABCC3 was downregulated after 6 hr of BUD and upregulated after 6 hr of the Co-T exposures. ABCC4 was upregulated after 5 hr of PM2.5 extract, BUD, and the Co-T exposures. The cytokine assay revealed an increase in IL-6 release by BEAS-2B exposed after 5 hr to PM2.5 extract, BUD, and the Co-T. At 7 hr, the Co-T decreases IL-6 release and IL-8 at 6 hr. In conclusion, the cotreatment showed an opposite effect on exposed BEAS-2B as compared with BUD. The results suggest an interference of the BUD therapeutic potential by PM2.5.http://dx.doi.org/10.1155/2017/6827194 |
| spellingShingle | Jarline Encarnación-Medina Rosa I. Rodríguez-Cotto Joseph Bloom-Oquendo Mario G. Ortiz-Martínez Jorge Duconge Braulio Jiménez-Vélez Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures Mediators of Inflammation |
| title | Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures |
| title_full | Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures |
| title_fullStr | Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures |
| title_full_unstemmed | Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures |
| title_short | Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM2.5, Budesonide, and Cotreated Exposures |
| title_sort | selective atp binding cassette subfamily c gene expression and proinflammatory mediators released by beas 2b after pm2 5 budesonide and cotreated exposures |
| url | http://dx.doi.org/10.1155/2017/6827194 |
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